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location United States
age 22
visits member for 1 year, 6 months
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Jul
17
accepted RNeasy Mini Kit low 260/230 ratio — can I purify this RNA for further use?
Jul
17
comment RNeasy Mini Kit low 260/230 ratio — can I purify this RNA for further use?
The low 260/230 ratio is indicative of guanidine contamination I believe. The RNeasy "RLT buffer" contains guanidine to my knowledge.
Jul
17
comment Do skull bones have pain nerves (nociceptors)?
Sure thing: mananatomy.com/basic-anatomy/nerve-supply-bones and ncbi.nlm.nih.gov/m/pubmed/12123694/as well as ncbi.nlm.nih.gov/m/pubmed/19425099
Jul
17
answered Do skull bones have pain nerves (nociceptors)?
Jul
16
comment RNeasy Mini Kit low 260/230 ratio — can I purify this RNA for further use?
These cells grow really slowly and I have some simple PCR that I need to run within the next couple days, so I'd ideally like to spare it. I've just never had these issues before. How do I precipitate the RNA using ethanol?
Jul
16
asked RNeasy Mini Kit low 260/230 ratio — can I purify this RNA for further use?
Jul
2
awarded  Curious
May
1
awarded  Tumbleweed
Apr
24
asked How does a TOPflash/FOPflash assay work to detect beta-catenin protein expression?
Jan
27
awarded  Popular Question
Jan
12
awarded  Yearling
Apr
1
accepted What metabolic activities are performed by a developing human fetus's liver?
Mar
26
comment What metabolic activities are performed by a developing human fetus's liver?
Thanks for the Google Books link ("develops its typical functions"). I was most curious about whether the fetal liver functions in response to nutrients obtained through the placenta, but this is the most informative source I've seen so far. Thank you!
Mar
23
comment Do kidneys have the enzyme 3-ketoacyl-CoA transferase?
This article's abstract says that 3-ketoacid-CoA transferase was found in the kidney: nature.com/pr/journal/v42/n4/abs/pr19972518a.html
Mar
23
asked What metabolic activities are performed by a developing human fetus's liver?
Mar
16
comment What is the biological mechanism underlying caffeine intolerance? (CYP1A2 or other?)
Yesterday I learned about this cool database linker site called biograph.be, where you can type in a gene and suspected "disease" or condition and it will generate a map showing how they've been associated in literature. Here's a start: biograph.be/concept/graph/C0006644/C1332826
Mar
16
comment Long term liquid food diet by blending/grinding all quality foods one would normaly eat, not any weight loss or prepared drinks
It would probably only be a positive thing -- ultimately, you're increasing surface area of the food particles, which is what our digestive systems try to do anyway in order to absorb the most nutrients.
Mar
16
asked If a receptor is inhibited throughout embryogenesis, could there be observable phenotypic differences in the adult?
Mar
16
awarded  Critic
Mar
2
accepted Question about cell signaling pathways (RTK, Jak-Stat, SMAD, etc)