474 reputation
113
bio website facebook.com/…
location United States
age 23
visits member for 1 year, 10 months
seen Nov 13 at 3:54

Oct
26
accepted Alternatives to trypsin for cell detachment?
Oct
17
comment Alternatives to trypsin for cell detachment?
Sounds simple enough, thanks for the tip.
Oct
17
comment Alternatives to trypsin for cell detachment?
My cells are in a T75 flask. They attach moderately strongly.
Oct
17
asked Alternatives to trypsin for cell detachment?
Oct
12
awarded  Custodian
Oct
12
reviewed Excellent Why has grey hair evolved?
Oct
12
reviewed Excellent What is the functional significance of the difference in cardiolipin/cholesterol ratio in different membranes?
Oct
12
reviewed Satisfactory How to estimate the DNA density in human sperm head?
Oct
12
answered What is the advantage to neurons being asymmetrical?
Jul
17
accepted RNeasy Mini Kit low 260/230 ratio — can I purify this RNA for further use?
Jul
17
comment RNeasy Mini Kit low 260/230 ratio — can I purify this RNA for further use?
The low 260/230 ratio is indicative of guanidine contamination I believe. The RNeasy "RLT buffer" contains guanidine to my knowledge.
Jul
17
comment Do skull bones have pain nerves (nociceptors)?
Sure thing: mananatomy.com/basic-anatomy/nerve-supply-bones and ncbi.nlm.nih.gov/m/pubmed/12123694/as well as ncbi.nlm.nih.gov/m/pubmed/19425099
Jul
17
answered Do skull bones have pain nerves (nociceptors)?
Jul
16
comment RNeasy Mini Kit low 260/230 ratio — can I purify this RNA for further use?
These cells grow really slowly and I have some simple PCR that I need to run within the next couple days, so I'd ideally like to spare it. I've just never had these issues before. How do I precipitate the RNA using ethanol?
Jul
16
asked RNeasy Mini Kit low 260/230 ratio — can I purify this RNA for further use?
Jul
2
awarded  Curious
May
1
awarded  Tumbleweed
Apr
24
asked How does a TOPflash/FOPflash assay work to detect beta-catenin protein expression?
Jan
27
awarded  Popular Question
Jan
12
awarded  Yearling