402 reputation
112
bio website facebook.com/…
location United States
age 22
visits member for 1 year, 6 months
seen 2 days ago

Jul
17
comment RNeasy Mini Kit low 260/230 ratio — can I purify this RNA for further use?
The low 260/230 ratio is indicative of guanidine contamination I believe. The RNeasy "RLT buffer" contains guanidine to my knowledge.
Jul
17
comment Do skull bones have pain nerves (nociceptors)?
Sure thing: mananatomy.com/basic-anatomy/nerve-supply-bones and ncbi.nlm.nih.gov/m/pubmed/12123694/as well as ncbi.nlm.nih.gov/m/pubmed/19425099
Jul
16
comment RNeasy Mini Kit low 260/230 ratio — can I purify this RNA for further use?
These cells grow really slowly and I have some simple PCR that I need to run within the next couple days, so I'd ideally like to spare it. I've just never had these issues before. How do I precipitate the RNA using ethanol?
Mar
26
comment What metabolic activities are performed by a developing human fetus's liver?
Thanks for the Google Books link ("develops its typical functions"). I was most curious about whether the fetal liver functions in response to nutrients obtained through the placenta, but this is the most informative source I've seen so far. Thank you!
Mar
23
comment Do kidneys have the enzyme 3-ketoacyl-CoA transferase?
This article's abstract says that 3-ketoacid-CoA transferase was found in the kidney: nature.com/pr/journal/v42/n4/abs/pr19972518a.html
Mar
16
comment What is the biological mechanism underlying caffeine intolerance? (CYP1A2 or other?)
Yesterday I learned about this cool database linker site called biograph.be, where you can type in a gene and suspected "disease" or condition and it will generate a map showing how they've been associated in literature. Here's a start: biograph.be/concept/graph/C0006644/C1332826
Mar
16
comment Long term liquid food diet by blending/grinding all quality foods one would normaly eat, not any weight loss or prepared drinks
It would probably only be a positive thing -- ultimately, you're increasing surface area of the food particles, which is what our digestive systems try to do anyway in order to absorb the most nutrients.
Mar
1
comment Question about cell signaling pathways (RTK, Jak-Stat, SMAD, etc)
see my comment on the post below -- I could have done a much better job saying what I was confused about in writing this question.
Mar
1
comment Question about cell signaling pathways (RTK, Jak-Stat, SMAD, etc)
Thank you, but I do understand that -- I suppose I didn't write my question very well and I was initially confused because my book explains the receptor tyrosine kinase (RTK) pathway in the context of the fibroblast growth factor family of paracrine factors, because the FGF receptors are RTK's. I was wondering how much of this "sharing" takes place -- how many of these ligands use the RTK pathway? My book implies that quite a few do, but isn't explicit about which.
Mar
1
comment Question about cell signaling pathways (RTK, Jak-Stat, SMAD, etc)
In contrast to each and every one that I listed having its own distinct pathway.
Feb
8
comment Simple diffusion of lipid-soluble molecules through phospholipid bilayer — does anything get “stuck” in transit?
Thank you -- I wasn't thinking about turnover and synthesis of new membrane lipids, and this is exactly what I needed. I thought for sure that at least theoretically a molecule could remain in between the bilayers, but I agree that the amount of this occurrence is relatively insignificant.
Feb
1
comment Why do cells have an average 'life span'
Henrietta Lacks had cervical cancer, and her cancerous cells somehow survived in culture (I don't know if this was due to proper handling by the lab staff or otherwise).
Feb
1
comment Why does trans fat decrease hdl's?
You might find this interesting: jn.nutrition.org/content/135/9/2075.full It doesn't directly answer your question, but it discusses potential mechanisms and is a good read in any case.
Jan
30
comment Which Receptors are Involved in the antidepressant effects of SSRIs?
I noticed you've asked a few questions about antidepressants recently--did you know that there's a cogsci stack that you can try too? I'm not saying these questions should be there instead of here, just saying you could ask in both places and see if it's helpful.
Jan
28
comment Why does trans fat decrease hdl's?
cis fats don't increase LDL. I think you've got them mixed up. Is your question why trans fats exert an effect on both HDL and LDL?
Jan
26
comment Fibonacci sequence in nature, truth or just wishful thinking?
I mean, I'm curious too, and Vi's video is all I've heard about this correlation. I'm not asserting she's right, just that you might enjoy it. The video is called Doodling in Math: Spirals, Fibonacci, and Being a Plant.
Jan
26
comment Fibonacci sequence in nature, truth or just wishful thinking?
Search for Vi Hart on Youtube. She makes educational and oddly entertaining math videos and she has one about plants displaying both Fibonacci and Lucas numbers as a way to maximize sunlight reception by the leaves.