871 reputation
419
bio website
location
age
visits member for 3 years
seen Aug 15 '13 at 16:22

Aug
16
comment Is the EC50 of an activating protein for an enzyme a good indicator for the binding affinity Kd?
bobthejoe: I like the detail in your answer, especially the hGH example is very surprising. However, my question pertains to comparing affinities and EC50 measurements, both being derived from in vitro assays with purified proteins. I would think the avidity argument might hold, especially when the same system were compared in a vesicular environment versus detergent solution.
Aug
16
revised Why do rod cells not recover after bleaching upon exposure to bright light?
Improved title
Aug
16
suggested approved edit on Why do rod cells not recover after bleaching upon exposure to bright light?
Aug
15
revised What gaseous substances do humans emit?
edited tags
Aug
14
revised (Rough) Model for DNA evolution in E. coli genes
Corrected taxonomy italicisation
Aug
14
reviewed Leave Open What is the molecular mechanism of cystine bond formation?
Aug
14
suggested approved edit on (Rough) Model for DNA evolution in E. coli genes
Aug
14
awarded  Quorum
Aug
13
revised Finding the number of chromosomes of an organism
Corrected taxonomy italicisation
Aug
13
suggested approved edit on Finding the number of chromosomes of an organism
Aug
13
answered How do parasitic fungi sense optimal fruiting conditions from inside the host?
Aug
12
awarded  Organizer
Aug
12
asked Is the EC50 of an activating protein for an enzyme a good indicator for the binding affinity Kd?
Aug
10
revised Is there a way for a 19th century scientist to prove that the octopus doesn't revive?
Added description of question
Aug
10
suggested approved edit on Is there a way for a 19th century scientist to prove that the octopus doesn't revive?
Aug
8
revised Is it possible for a substance to be absorbed by the hair or the scalp?
edited grammar
Aug
8
suggested approved edit on Is it possible for a substance to be absorbed by the hair or the scalp?
Aug
6
comment Absorption ratios 260/280 and 260/230 for RNA
For the sake of completeness and relevant detail: The aromatic amino acids (Phe, Trp and Tyr) are the primary contributors to the ability of proteins to absorb at 280nm. Cysteine double bonds can also absorb but its both rare and the extinction coefficient is smaller in comparison to the aromatic residues.
Aug
4
comment What is itching?
I have seen two answers posted and then "disappear" after a couple of days.. Can someone tell me whats happening or was I dreaming? :-)
Aug
2
revised Absorption ratios 260/280 and 260/230 for RNA
Corrected numbers, both ratios were labeled "260/280"