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Aug
21
revised How to find ion/water channel related genes
edited tags
Aug
21
revised How to find ion/water channel related genes
edited tags
Aug
21
comment What makes a wet dog so stinky?
The "after rain" smell is technically called "petrichor". In summary, compounds from plants emitted during dry periods get absorbed into rocks. Rain releases these compounds and the smell derives from these compounds. The wikipedia page is a good place to read more about petrichor.
Aug
20
comment Why is GTP gamma S non hydrolyzable?
I'm almost certain its got something to do with the amino acid in the P-loop that contacts the gamma phosphate, however without a reference I am not going to post it as an answer yet. I can ask my PI but then its more fun to find it out myself ;)
Aug
20
asked Why is GTP gamma S non hydrolyzable?
Aug
20
accepted Is the EC50 of an activating protein for an enzyme a good indicator for the binding affinity Kd?
Aug
20
comment Is the EC50 of an activating protein for an enzyme a good indicator for the binding affinity Kd?
bobthejoe, just thought I'd say this: You've laid out really well why interpreting Km is not always trivial. Exactly why I chose to focus on EC50 (with the trivial(?) assumption that substrate far exceeds enzyme concentration). I'm going to accept your answer, given the richness of detail that you've laid out!
Aug
16
comment Is the EC50 of an activating protein for an enzyme a good indicator for the binding affinity Kd?
bobthejoe: I like the detail in your answer, especially the hGH example is very surprising. However, my question pertains to comparing affinities and EC50 measurements, both being derived from in vitro assays with purified proteins. I would think the avidity argument might hold, especially when the same system were compared in a vesicular environment versus detergent solution.
Aug
16
revised Why do rod cells not recover after bleaching upon exposure to bright light?
Improved title
Aug
16
suggested approved edit on Why do rod cells not recover after bleaching upon exposure to bright light?
Aug
15
revised What gaseous substances do humans emit?
edited tags
Aug
14
revised (Rough) Model for DNA evolution in E. coli genes
Corrected taxonomy italicisation
Aug
14
reviewed Leave Open What is the molecular mechanism of cystine bond formation?
Aug
14
suggested approved edit on (Rough) Model for DNA evolution in E. coli genes
Aug
14
awarded  Quorum
Aug
13
revised Finding the number of chromosomes of an organism
Corrected taxonomy italicisation
Aug
13
suggested approved edit on Finding the number of chromosomes of an organism
Aug
13
answered How do parasitic fungi sense optimal fruiting conditions from inside the host?
Aug
12
awarded  Organizer
Aug
12
asked Is the EC50 of an activating protein for an enzyme a good indicator for the binding affinity Kd?