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visits member for 2 years, 4 months
seen Aug 15 '13 at 16:22

Aug
2
awarded  Nice Question
Aug
1
asked What is itching?
Jul
30
comment How would I explain different properties of the same protein in different species?
The NCBI protein database or UniProtKB would be the first places I would search. Other databases exist, but these are my personal favourites
Jul
30
comment How would I explain different properties of the same protein in different species?
Have you checked sequence similarity/identity, especially of the nucleotide contacting regions (otherwise known as the P-loops I think)?
Jul
24
comment How to compute properties of peptides ?
The introductory chapter of Proteins by Thomas Creighton (books.google.com/books/about/Proteins.html?id=hu8T_kI1LrkC) would be a great place to start. It was my Bible for introductory biochemistry in college!
Jul
22
answered What role does a protein's size have on protein-protein interactions?
Jul
12
awarded  Citizen Patrol
Jul
3
awarded  Commentator
Jul
1
comment How do I deal with sticky and viscous samples from cell lysates?
Definitely your suggestions will make things much better. I think Mad Scientist was looking for a quick and dirty way to do it when doing small scale checks for protein expression from a new construct.
Jun
28
answered How do I deal with sticky and viscous samples from cell lysates?
May
18
awarded  Critic
May
16
accepted What is the biology behind a skin “mole”?
May
16
awarded  Nice Question
May
10
comment Do gene expression levels necessarily correspond to levels of protein activation?
@Daniel: +1 for ending it at "..high throughput (which is all the rage these days)"!
May
10
revised Protein construct design
added details
May
10
comment Protein construct design
I used the x-ray structure to determine known loop regions that connect structured domain regions (pt #1)
May
10
asked Protein construct design
May
10
comment How does a cell know its size?
Of particular interest to this question might be the latest issue of Curr Biol that has a special section devoted to this topic: Curr Biol, Vol 22 Issue 9 (cell.com/current-biology/issue?pii=S0960-9822%2812%29X0009-5)
May
3
comment Generating custom human DNA sequences based on traits such as eye colour?
"maybe" is the keyword ;-)
Apr
30
comment Does anyone have any TOPO directional cloning tips?
From what I read, @Ben seems to be saying that he is not looking to troubleshoot the original (traditional) method which failed and is only looking for tips on using the TOPO kit as a first-timer..