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Aug
6
comment Absorption ratios 260/280 and 260/230 for RNA
For the sake of completeness and relevant detail: The aromatic amino acids (Phe, Trp and Tyr) are the primary contributors to the ability of proteins to absorb at 280nm. Cysteine double bonds can also absorb but its both rare and the extinction coefficient is smaller in comparison to the aromatic residues.
Aug
4
comment What is itching?
I have seen two answers posted and then "disappear" after a couple of days.. Can someone tell me whats happening or was I dreaming? :-)
Aug
2
revised Absorption ratios 260/280 and 260/230 for RNA
Corrected numbers, both ratios were labeled "260/280"
Aug
2
suggested approved edit on Absorption ratios 260/280 and 260/230 for RNA
Aug
2
awarded  Nice Question
Aug
1
asked What is itching?
Jul
30
comment How would I explain different properties of the same protein in different species?
The NCBI protein database or UniProtKB would be the first places I would search. Other databases exist, but these are my personal favourites
Jul
30
comment How would I explain different properties of the same protein in different species?
Have you checked sequence similarity/identity, especially of the nucleotide contacting regions (otherwise known as the P-loops I think)?
Jul
24
comment How to compute properties of peptides ?
The introductory chapter of Proteins by Thomas Creighton (books.google.com/books/about/Proteins.html?id=hu8T_kI1LrkC) would be a great place to start. It was my Bible for introductory biochemistry in college!
Jul
22
answered What role does a protein's size have on protein-protein interactions?
Jul
12
awarded  Citizen Patrol
Jul
3
awarded  Commentator
Jul
1
comment How do I deal with sticky and viscous samples from cell lysates?
Definitely your suggestions will make things much better. I think Mad Scientist was looking for a quick and dirty way to do it when doing small scale checks for protein expression from a new construct.
Jun
28
answered How do I deal with sticky and viscous samples from cell lysates?
May
18
awarded  Critic
May
16
accepted What is the biology behind a skin “mole”?
May
16
awarded  Nice Question
May
10
comment Do gene expression levels necessarily correspond to levels of protein activation?
@Daniel: +1 for ending it at "..high throughput (which is all the rage these days)"!
May
10
revised Protein construct design
added details
May
10
comment Protein construct design
I used the x-ray structure to determine known loop regions that connect structured domain regions (pt #1)