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Oct
21
comment Can I image Coomassie and GFP in gels at the same time with a fluorescence scanner?
@leonardo the standard running/gel/loading buffers do not seem to be so aggressive as to destroy (all) the GFP; it may be denatured in the loading dye, but when washed out into the gel buffer it seems to recover. On the other hand, putting the gel into destain or fixing solution is very effective at obliterating the fluorescence.
Jul
13
comment Does cockroach lay eggs in human flesh when they “bite”?
Because hydroxide solutions are not usually for-sale unless you're looking in the plumbing aisle.
Jul
12
comment Does cockroach lay eggs in human flesh when they “bite”?
I assume you mean peroxide solution (hydrogen peroxide); not hydroxide (e.g. sodium hydroxide, a strong base).
Jul
12
revised Why does Penicillin only affect bacterial cell walls
eszett -> beta
Jul
12
accepted How do I change bond colors to something besides a different hue in Coot?
Jul
12
accepted How do you turn a minimal CIF description into a complete one?
Jul
12
accepted How can I pare down a PDB file in Python to only include specific residues?
Jul
12
accepted How should I ship plasmids?
Jul
12
asked Can I image Coomassie and GFP in gels at the same time with a fluorescence scanner?
Jun
21
comment Turn off multithreading in pymol
@DanielStandage So on the (fictional) Builders.SE site, "How do I fix my circular saw?" should be closed because it's not about building anything?
Jun
5
awarded  Necromancer
May
28
awarded  Nice Answer
May
16
answered Can protein structure be determined by X-Ray Diffraction in a single image?
Apr
25
answered Do trees really get a large share of their mass from the carbon in the air?
Apr
12
asked How do I prepare a PDB for submission to the Protein Data Bank?
Apr
2
awarded  Self-Learner
Apr
1
comment How can I pare down a PDB file in Python to only include specific residues?
@David unfamiliar with that site, if you think it's better there, feel free to flag a mod down.
Mar
29
comment What is the advantage of using starter cultures for growing bacteria?
I'm not sure if I'm reading into this incorrectly, but does E. coli (or monocultures in general) function commensally? e.g., if I inoculate 5 mL or 1 L with the same number of cells, will the 5 mL produce more cells (not density) faster?
Mar
29
revised How can I pare down a PDB file in Python to only include specific residues?
added 307 characters in body
Mar
29
revised How can I pare down a PDB file in Python to only include specific residues?
added 664 characters in body