Nick T

1,457 reputation

422

bio	website	None
	location	Chicago, IL
	age	27
visits	member for	1 year, 5 months
	seen	Oct 21 '12 at 18:11
stats	profile views	36

Graduate student at Northwestern University studying bio sciences; still know my electrical engineering though.

Learning:
- Python (+ wxPython, PIL Image, others),
- PowerShell
- Java (on Android platform)
Know C on embedded well (what's a file? stream? :P)
Used to know HTML (sort-of now), CSS (less-so...), JScript (no.) At least I was able to forget most of this stuff just in time for AJAX to become useful. (Because it's totally different from DHTML...)

I like stout, esp. oatmeal stout. Gin and tonic if we're talking mixed drinks. Also like Portal (hell, all Valve games), and would kill a bus full of baby nuns with fire if Gabe would release Half-Life 3 already.

	1,457 reputation	bio	website	None	visits	member for	1 year, 5 months
422 badges		location	Chicago, IL		seen	Oct 21 '12 at 18:11

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Dec 20	comment	How do I prepare and clone from E. coli DNA? @JamesPan feel free to post another answer; your experience and depth of knowledge seems better than mine. The more answers, the better.
Dec 20	comment	How do I prepare and clone from E. coli DNA? @JamesPan I've subcloned genes between vectors, I just didn't know if there was any differences between vector->vector and genome->vector. It's all DNA so in theory there isn't anything new, but I don't know about the practical considerations.
Dec 17	answered	How do I prepare and clone from E. coli DNA?
Dec 17	answered	What determines a successful protein expression in E. coli?
Dec 17	comment	How do the brain and nerves create electrical pulses? @Dr.Dredel please see the discussion on meta and comment there
Dec 17	comment	What are good resources for self-learning modern molecular biology concepts? @Poshpaws community wiki is not a crutch for extremely subjective questions.
Dec 16	revised	How can I produce milligram quantities of an isotope-labeled DNA oligomer? rephrase title to better summarize post, sp fix tag
Dec 16	comment	What are good resources for self-learning modern molecular biology concepts? I've voted to close because this (the title much more-so than the rest) is very subjective so doesn't have a "right" answer. If you can narrow the scope to a particular issue it would be much improved.
Dec 16	comment	How do the brain and nerves create electrical pulses? This question has brought up a discussion on meta.
Dec 16	suggested	suggested edit on How can I produce milligram quantities of an isotope-labeled DNA oligomer?
Dec 15	comment	How long does antibiotic-dosed LB maintain good selection? Ampicillin degrades by hydrolysis (albeit very slowly if not catalyzed), difficult when in dry, much easier when in solution.
Dec 15	awarded	Critic
Dec 15	revised	How long does antibiotic-dosed LB maintain good selection? inline links, link directly to study, specifically state study findings
Dec 15	comment	Do large animals often evolve into smaller animals? Define "often"; island dwarfism is a seemingly-repeatable effect.
Dec 15	comment	How to understand influenza strain designations? @J.M. if there was any particular virus deserving of the tag, the flu would be one.
Dec 15	awarded	Mortarboard
Dec 15	comment	How is Taq polymerase produced? @nico I don't know, clearly someone wants them or they wouldn't be offered. I've only done a couple PCR reactions, and only with Phusion (Pyrococcus derived; "50X" fidelity)
Dec 15	suggested	suggested edit on How long does antibiotic-dosed LB maintain good selection?
Dec 15	awarded	Nice Question
Dec 15	comment	How is Taq polymerase produced? It certainly has been, that's just an example demonstrating it's ability to be artificially cultured. Biotech companies that produce native Taq probably have all sorts of tricks and optimizations that they'd never share.