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Dec
17
comment What are good resources for self-learning modern molecular biology concepts?
@Poshpaws community wiki is not a crutch for extremely subjective questions.
Dec
16
revised How can I produce milligram quantities of an isotope-labeled DNA oligomer?
rephrase title to better summarize post, sp fix tag
Dec
16
comment What are good resources for self-learning modern molecular biology concepts?
I've voted to close because this (the title much more-so than the rest) is very subjective so doesn't have a "right" answer. If you can narrow the scope to a particular issue it would be much improved.
Dec
16
comment How do the brain and nerves create electrical pulses?
This question has brought up a discussion on meta.
Dec
16
suggested approved edit on How can I produce milligram quantities of an isotope-labeled DNA oligomer?
Dec
15
comment How long does antibiotic-dosed LB maintain good selection?
Ampicillin degrades by hydrolysis (albeit very slowly if not catalyzed), difficult when in dry, much easier when in solution.
Dec
15
awarded  Critic
Dec
15
revised How long does antibiotic-dosed LB maintain good selection?
inline links, link directly to study, specifically state study findings
Dec
15
comment Do large animals often evolve into smaller animals?
Define "often"; island dwarfism is a seemingly-repeatable effect.
Dec
15
comment How to understand influenza strain designations?
@J.M. if there was any particular virus deserving of the tag, the flu would be one.
Dec
15
awarded  Mortarboard
Dec
15
comment How is Taq polymerase produced?
@nico I don't know, clearly someone wants them or they wouldn't be offered. I've only done a couple PCR reactions, and only with Phusion (Pyrococcus derived; "50X" fidelity)
Dec
15
suggested approved edit on How long does antibiotic-dosed LB maintain good selection?
Dec
15
awarded  Nice Question
Dec
15
comment How is Taq polymerase produced?
It certainly has been, that's just an example demonstrating it's ability to be artificially cultured. Biotech companies that produce native Taq probably have all sorts of tricks and optimizations that they'd never share.
Dec
15
awarded  Commentator
Dec
15
comment Life without DNA?
The question of them being alive is moot; prions are ultimately dependent on DNA for their propagation as the host must generate additional substrate for them to turn over.
Dec
15
comment Life without DNA?
Irregardless if viruses and prions are "alive", those that we know cannot survive in a word without RNA/DNA.
Dec
15
comment How is Taq polymerase produced?
There are protocols on how to culture T. aquaticus, but I don't see any on how to get any decent expression of Taq pol. Maybe it expresses extremely well anyways? It still seems asinine that companies charge a mere ~10% price premium on the native vs. recombinant protein; probably why a startup that is sharing our building is able to carve themselves out a market.
Dec
15
revised How long does antibiotic-dosed LB maintain good selection?
deleted 9 characters in body