1,570 reputation
824
bio website None
location Chicago, IL
age 28
visits member for 3 years
seen Nov 24 at 14:32

Graduate student at Northwestern University studying bio sciences and BIGG DATAAAAA; still know my electrical engineering though.

  • Learning:
    • Python (+ wxPython, PIL Image, others),
    • PowerShell
    • Java (on Android platform)
  • Know C on embedded well (what's a file? stream? :P)
  • Used to know HTML (sort-of now), CSS (less-so...), JScript (no.) At least I was able to forget most of this stuff just in time for AJAX to become useful. (Because it's totally different from DHTML...)

I like stout, esp. oatmeal stout. Gin and tonic if we're talking mixed drinks. Also like Portal (hell, all Valve games), and would kill a bus full of baby nuns with fire if Gabe would release Half-Life 3 already.


Dec
15
comment Which sequence characteristics influence the transcription efficiency of T7 polymerase?
Would be nice to know why some of my T7-promoter plasmids produce ~100 mg protein/L of culture and others maybe 2-3.
Dec
15
awarded  Quorum
Dec
15
revised What is a good miniprep protocol for the class room?
fix tag misspelling, include links in-line vs. tacked on at the end
Dec
15
suggested approved edit on What is a good miniprep protocol for the class room?
Dec
15
comment What is a good miniprep protocol for the class room?
All the prep kits I've used (Qiagen, Promega, Invitrogen) do use alkaline lysis. I like the columns just for the sake of not having to worry about totally evaporating all the ethanol (I use them with a vacuum manifold so I suck for an extra minute or two to let it all evaporate) or looking for some half-invisible pellet.
Dec
15
answered How to understand influenza strain designations?
Dec
15
awarded  Editor
Dec
15
revised How can I avoid digesting protein-bound DNA?
make title represent question better, reorder body to place the goal first followed by question.
Dec
15
revised What are the limiting factors for gene length and number of exons?
phrase title as a question
Dec
14
awarded  Teacher
Dec
14
suggested approved edit on How can I avoid digesting protein-bound DNA?
Dec
14
suggested approved edit on What are the limiting factors for gene length and number of exons?
Dec
14
answered What specific membrane adaptations do cells have for saline-rich environs?
Dec
14
answered What is a good miniprep protocol for the class room?
Dec
14
awarded  Precognitive
Dec
14
revised How long does antibiotic-dosed LB maintain good selection?
edited tags
Dec
14
revised How is RNAse contamination in RNA based experiments prevented?
edited tags
Dec
14
awarded  Organizer
Dec
14
asked How should I ship plasmids?
Dec
14
awarded  Supporter