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awarded  Popular Question
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answered Is this a valid definition of independent assortment?
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awarded  Nice Question
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accepted E coli cotransformation efficiency
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awarded  Nice Question
Jul
29
revised E coli cotransformation efficiency
make the question more prone to get detailed answer on efficiency of co-transformation, and not so much on explanation on how to co-transform (by double selection)
Jul
29
comment E coli cotransformation efficiency
Thanks, Chris, but I was more wondering how high with efficiency (number of clones per ug DNA) you can get with co-transformation. For example for single electroporation the efficiency is around 1 × 10^10 cfu/μg so I would like to know what is the limit and whether it can be improved for co-transformation.
Jul
10
comment Efficiency of plasmid DNA isolation from frozen E. coli cell cultures
Thanks Chris. I tried it with 25ml cultures and I got great yields! I did not run them on a gel to check the quality of the DNA, but the sequencing results looked good!
Jul
10
accepted Efficiency of plasmid DNA isolation from frozen E. coli cell cultures
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asked Efficiency of plasmid DNA isolation from frozen E. coli cell cultures
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