4,602 reputation
11147
bio website linkedin.com/profile/…
location Stanford, CA
age 28
visits member for 3 years
seen Nov 11 at 22:21

Biochemistry PhD student at Stanford University. Interested in natural products engineering and synthetic biology.


Dec
14
awarded  Yearling
Oct
30
answered Is this a valid definition of independent assortment?
Oct
27
awarded  Nice Question
Oct
23
awarded  Nice Question
Sep
30
awarded  Explainer
Sep
17
awarded  Nice Answer
Sep
3
awarded  Nice Answer
Aug
21
accepted E coli cotransformation efficiency
Aug
10
awarded  Nice Question
Jul
29
revised E coli cotransformation efficiency
make the question more prone to get detailed answer on efficiency of co-transformation, and not so much on explanation on how to co-transform (by double selection)
Jul
29
comment E coli cotransformation efficiency
Thanks, Chris, but I was more wondering how high with efficiency (number of clones per ug DNA) you can get with co-transformation. For example for single electroporation the efficiency is around 1 × 10^10 cfu/μg so I would like to know what is the limit and whether it can be improved for co-transformation.
Jul
10
comment Efficiency of plasmid DNA isolation from frozen E. coli cell cultures
Thanks Chris. I tried it with 25ml cultures and I got great yields! I did not run them on a gel to check the quality of the DNA, but the sequencing results looked good!
Jul
10
accepted Efficiency of plasmid DNA isolation from frozen E. coli cell cultures
Jul
10
awarded  Nice Answer
Jul
8
asked Efficiency of plasmid DNA isolation from frozen E. coli cell cultures
Jul
2
awarded  Curious
Jun
2
answered What is the definition of a stringent/relaxed plasmid?
Apr
9
awarded  Taxonomist
Apr
1
answered Is a sequential double transformation acceptable?
Mar
28
asked E coli cotransformation efficiency