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Apr
28
comment de novo gene synthesis vs cDNA library creation from plant RNA: Pros & Cons
Isn't "collecting plant samples, cryopreservation, digestion, extraction etc." also expensive? Think about cost and time. How long would it take to isolate the DNA vs synthesize it?
Apr
28
asked T7 vs pBAD promoter strength
Apr
26
comment What is the highest number of genes expressed under one T7 promoter?
@aaaaaa Not really, since I would like to avoid adding extra sequence to the coding region.
Apr
26
answered What is a regulable promoter? And how does one regulate it?
Apr
26
comment Why does pETDuet-1 only have one T7 terminator but two T7 promoters?
@WYSIWYG Added vector map. The genes are not overlapping.
Apr
26
revised Why does pETDuet-1 only have one T7 terminator but two T7 promoters?
added vector map
Apr
26
comment Why does pETDuet-1 only have one T7 terminator but two T7 promoters?
@Jory But in that case why is there a second promoter?
Apr
25
answered Why is DNA Shuffling more efficient than Point Mutation?
Apr
25
revised Why does pETDuet-1 only have one T7 terminator but two T7 promoters?
edited tags
Apr
25
revised What is the highest number of genes expressed under one T7 promoter?
edited tags
Apr
25
comment Why does pETDuet-1 only have one T7 terminator but two T7 promoters?
Not sure it can. But waiting from reply from them as well.
Apr
25
asked What is the highest number of genes expressed under one T7 promoter?
Apr
25
asked Why does pETDuet-1 only have one T7 terminator but two T7 promoters?
Apr
17
comment Online multiple sequence alignment with constraints
I also don't understand your constraints. Also, why do you need constraints? Usually when you do MSA, you don't pose any constraints since you presumably don't know how the query sequences can be aligned. Also, I bet that if you include sequence "p" in your MSA, the alignment will be different and will include your constraints.
Apr
16
awarded  molecular-biology
Apr
15
comment Can one talk about deacetylation of a promoter rather than associated histone?
I would say yes, it is probably acceptable, but I agree that the authors should have been more clear by stating that changing the acetylation state of the histones wrapping the promoter affects the expression of the gene from that promoter.
Apr
15
comment Can one talk about deacetylation of a promoter rather than associated histone?
The promoter sequence is wrapped by histones, which a modified (look at figure 3). "KLF11 binds distinct nuclear cofactor complexes that posttranslationally modify promoter histones to epigenetically alter the chromatin state, gene expression levels, and activity"
Apr
15
answered Are there proteins that stabilize mRNA:RNA polymerase or mRNA:ribosome complex?
Apr
15
awarded  Popular Question
Apr
13
answered Directed evolution: Point mutation vs Insertion-Deletion vs Shuffling