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location Stanford, CA
age 28
visits member for 3 years
seen Nov 11 at 22:21

Biochemistry PhD student at Stanford University. Interested in natural products engineering and synthetic biology.


Jan
12
comment Reverse transcription PCR optimization
@Alexander Galkin Yes, I believe the question is more about PCR rather than RT. You are probably right that RT-PCR tag is too specific. The PCR tag is good enough.
Jan
12
revised Reverse transcription PCR optimization
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Jan
12
comment Reverse transcription PCR optimization
@Alexander Galkin Why did you remove the PCR tag? I think the question is really about RT PCR, and not RT in particular.
Jan
12
comment Why are amino acids in biology homochiral?
Hope these papers are helpful. Let me know what you have found.
Jan
12
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Jan
12
revised Reverse transcription PCR optimization
You don't have to give the detais of your question in its title
Jan
12
comment Absorption ratios 260/280 and 260/230 for RNA
If you have two peaks is a clear indication of contamination. Don't look only at the ratios because this gives you some idea, but doesn't make a strong statement. The PCR might fail due to contamination but you might try it anyway. You might also try isolating your RNA again.
Jan
12
suggested approved edit on Reverse transcription PCR optimization
Jan
12
comment Reverse transcription PCR optimization
RT PCR refers to reverse transcription I assume? Not to real time PCR?
Jan
12
awarded  Organizer
Jan
12
revised Absorption ratios 260/280 and 260/230 for RNA
edited tags
Jan
12
revised Absorption ratios 260/280 and 260/230 for RNA
edited tags
Jan
12
answered Absorption ratios 260/280 and 260/230 for RNA
Jan
12
revised translation wiki excerpt
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Jan
12
revised
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Jan
12
answered Why are amino acids in biology homochiral?
Jan
12
answered What are the different ways an exon gets spliced?
Jan
11
suggested approved edit on
Jan
11
wiki