| bio | website | linkedin.com/profile/… |
|---|---|---|
| location | Palo Alto, CA | |
| age | 27 | |
| visits | member for | 1 year, 6 months |
| seen | Jun 4 at 2:51 | |
| stats | profile views | 199 |
Visiting scholar at Stanford Genome Technology Center. Interested in synthetic biology.
|
Oct 15 |
comment |
How are the boundaries of a gene determined? @RichardSmith They are basically looking for start codons (ATG, GTG) that define the beginning of the open reading frame (ORF), and stop codons (TAG, TAA, TGA), that define the end of the ORF, and also checks whether the number of bases between the start codon and the stop codon is devisible by 3. |
|
Sep 21 |
awarded | Custodian |
|
Sep 17 |
comment |
Are human fetuses more likely to be male? @nico: Done, thanks. |
|
Sep 17 |
revised |
Are human fetuses more likely to be male? added 42 characters in body |
|
Sep 8 |
awarded | Nice Answer |
|
Jul 3 |
comment |
How would one describe the R-factor in crystallography? And what is the background of the person you would like to explain the R-factor concept to? |
|
Jun 29 |
comment |
T7 promoter leakiness Isn't it possible that a regular E. coli strain that does not have T7 polymerase, has other polymerases that can recognize the T7 promoter? |
|
Jun 27 |
revised |
T7 promoter leakiness added 230 characters in body |
|
Jun 27 |
asked | T7 promoter leakiness |
|
Jun 15 |
awarded | Nice Question |
|
Jun 15 |
comment |
Designing genes with DNAWorks: Maximum nonzero score? @bobthejoe I am trying to say that the design portion is not the bottleneck. |
|
Jun 15 |
answered | Designing genes with DNAWorks: Maximum nonzero score? |
|
Jun 14 |
comment |
Designing genes with DNAWorks: Maximum nonzero score? Can you give us more information on what you are trying to assemble (size, length of overlap, length of oligos) and how you plan to assemble it? |
|
May 26 |
comment |
Co-transformation of plasmids from the same incompatibility group Thanks for your answer. I actually co-transformed and I got twice as many colonies compared to a uni-transformation. I think there might me something wrong with the plates or the cells, but perhaps the co-transformation is more efficient. Which I still don't understand why would that be.... |
|
May 23 |
asked | Co-transformation of plasmids from the same incompatibility group |
|
May 15 |
revised |
Restriction Mapping of Plasmid Assignment added 2845 characters in body |
|
May 13 |
revised |
Restriction Mapping of Plasmid Assignment added 41 characters in body |
|
May 13 |
answered | Restriction Mapping of Plasmid Assignment |
|
Apr 27 |
asked | Why is PEG important for efficient yeast transformation? |
|
Apr 25 |
answered | Primer design for introduction of restriction sites flanking a gene of interest |