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Mar
5
comment How do mosquitoes maintain telomere length?
No I am not but I know the principle. You should just read this paper and try see whether you believe the interpretation of their data. Also just wait to see what other people might say about that.
Mar
5
comment How do mosquitoes maintain telomere length?
mm that's a good question. My first thought would be no, as eukaryotic cells are in general capable of recombination. I am not really familiar with mosquitoes, but would assume that applies for them as well. But again, it might be possible that there are telomere-specific recombination enzymes. Worth looking into.
Mar
5
answered How do mosquitoes maintain telomere length?
Mar
5
comment Plasmid maintenance
Yeah, I think this is a good backup.
Mar
5
revised How many transcription factors are there?
added 95 characters in body
Mar
5
answered How many transcription factors are there?
Mar
5
answered Plasmid maintenance
Mar
5
comment Using PSI-BLAST
Yes, each time you iterate a new PSSM is formed. You might want to use it if you would want to identify homologs that are undetectable by simple BLAST.
Mar
5
revised Using PSI-BLAST
added 1 characters in body
Mar
5
answered Using PSI-BLAST
Mar
4
answered What is the relation between plasmid concentration and mRNA levels?
Mar
3
comment Does the MS2 RNA binding protein have any translational repression effects?
Oh I see now... Interesting idea. Worth exploring. Why don't you use Cas9?
Feb
27
reviewed Approve What's the difference between an embryo and a fetus?
Feb
27
answered What's the difference between an embryo and a fetus?
Feb
25
comment What is the fastest way to build an alanine scanning library?
Are you still interested in the answer of this question?
Feb
25
revised Does the MS2 RNA binding protein have any translational repression effects?
Adding references and a figure
Feb
25
answered Does the MS2 RNA binding protein have any translational repression effects?
Feb
25
comment Does the MS2 RNA binding protein have any translational repression effects?
Why don't you place the MS2L downstream of the ORF? What exactly is the experiment you want to do?
Feb
25
comment What are the effect of microtubule or microfilament inhibition on yeast expression profile?
Thanks for the answer. Could you summarize what was discovered? Which genes were downregulated/upregulated/unchanged? Thanks.
Feb
25
asked What are the effect of microtubule or microfilament inhibition on yeast expression profile?