4,283 reputation
11039
bio website linkedin.com/profile/…
location Stanford, CA
age 28
visits member for 2 years, 4 months
seen 23 hours ago

Biochemistry PhD student at Stanford University. Interested in natural products engineering and synthetic biology.


Nov
11
asked Recombinant protein fraction in E. coli
Oct
15
comment How are the boundaries of a gene determined?
@ghchinoy Yes, that could be interesting, but I don't think it is more complicated than I already explained to Richard. You can of course add a few more "checks" the software can do, like the length of the ORF.
Oct
15
comment How are the boundaries of a gene determined?
@RichardSmith They are basically looking for start codons (ATG, GTG) that define the beginning of the open reading frame (ORF), and stop codons (TAG, TAA, TGA), that define the end of the ORF, and also checks whether the number of bases between the start codon and the stop codon is devisible by 3.
Sep
21
awarded  Custodian
Sep
17
comment Are human fetuses more likely to be male?
@nico: Done, thanks.
Sep
17
revised Are human fetuses more likely to be male?
added 42 characters in body
Sep
8
awarded  Nice Answer
Jul
3
comment How would one describe the R-factor in crystallography?
And what is the background of the person you would like to explain the R-factor concept to?
Jun
29
comment T7 promoter leakiness
Isn't it possible that a regular E. coli strain that does not have T7 polymerase, has other polymerases that can recognize the T7 promoter?
Jun
27
revised T7 promoter leakiness
added 230 characters in body
Jun
27
asked T7 promoter leakiness
Jun
15
awarded  Nice Question
Jun
15
comment Designing genes with DNAWorks: Maximum nonzero score?
@bobthejoe I am trying to say that the design portion is not the bottleneck.
Jun
15
answered Designing genes with DNAWorks: Maximum nonzero score?
Jun
14
comment Designing genes with DNAWorks: Maximum nonzero score?
Can you give us more information on what you are trying to assemble (size, length of overlap, length of oligos) and how you plan to assemble it?
May
26
comment Co-transformation of plasmids from the same incompatibility group
Thanks for your answer. I actually co-transformed and I got twice as many colonies compared to a uni-transformation. I think there might me something wrong with the plates or the cells, but perhaps the co-transformation is more efficient. Which I still don't understand why would that be....
May
23
asked Co-transformation of plasmids from the same incompatibility group
May
15
revised Restriction Mapping of Plasmid Assignment
added 2845 characters in body
May
13
revised Restriction Mapping of Plasmid Assignment
added 41 characters in body
May
13
answered Restriction Mapping of Plasmid Assignment