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Dec
14
awarded  Yearling
Dec
10
comment Can compounds be predicted from Polyketide synthases gene clusters?
Each module adds a two (not three) carbon extender unit to the growing polyketide chain. Pleas correct it in your text.
Dec
10
comment Can compounds be predicted from Polyketide synthases gene clusters?
I would love to talk to you about this subject after my final :-) Why are you interested?
Nov
26
asked How are epigenetic marks transmitted during cell division?
Nov
22
revised Cell growth conditions for preparing electrocompetent cells
edited title
Nov
22
asked Cell growth conditions for preparing electrocompetent cells
Nov
11
comment Recombinant protein fraction in E. coli
I also had a feeling that the protein concentration should be more uniform, especially for the same protein (GFP in this case). I also imagined it would not be lower than 40%.....
Nov
11
asked Recombinant protein fraction in E. coli
Oct
15
comment How are the boundaries of a gene determined?
@ghchinoy Yes, that could be interesting, but I don't think it is more complicated than I already explained to Richard. You can of course add a few more "checks" the software can do, like the length of the ORF.
Oct
15
comment How are the boundaries of a gene determined?
@RichardSmith They are basically looking for start codons (ATG, GTG) that define the beginning of the open reading frame (ORF), and stop codons (TAG, TAA, TGA), that define the end of the ORF, and also checks whether the number of bases between the start codon and the stop codon is devisible by 3.
Sep
21
awarded  Custodian
Sep
17
comment Are human fetuses more likely to be male?
@nico: Done, thanks.
Sep
17
revised Are human fetuses more likely to be male?
added 42 characters in body
Sep
8
awarded  Nice Answer
Jul
3
comment How would one describe the R-factor in crystallography?
And what is the background of the person you would like to explain the R-factor concept to?
Jun
29
comment T7 promoter leakiness
Isn't it possible that a regular E. coli strain that does not have T7 polymerase, has other polymerases that can recognize the T7 promoter?
Jun
27
revised T7 promoter leakiness
added 230 characters in body
Jun
27
asked T7 promoter leakiness
Jun
15
awarded  Nice Question
Jun
15
comment Designing genes with DNAWorks: Maximum nonzero score?
@bobthejoe I am trying to say that the design portion is not the bottleneck.