4,450 reputation
11041
bio website linkedin.com/profile/…
location Stanford, CA
age 28
visits member for 2 years, 7 months
seen 16 hours ago

Biochemistry PhD student at Stanford University. Interested in natural products engineering and synthetic biology.


Mar
18
awarded  Nice Answer
Mar
17
reviewed Approve suggested edit on What are the evolutionary niches differentiating an apple from a pear?
Mar
16
awarded  Nice Question
Mar
14
comment What is causing my problem with very low yields when isolating a 42kb yeast plasmid?
What should be the OD of the culture? What is the volume of the culture?
Mar
14
revised Which is more important for protein expression mRNA structure or codon optimization?
added 3 characters in body
Mar
14
comment Which is more important for protein expression mRNA structure or codon optimization?
I spent quite some time trying to answer the same question, so I couldn't help but share my knowledge!
Mar
14
comment Is there an enzyme for the transformation of the hydroxyl group?
Can you give us some background? What is your aromatic compound? Why do you want to avoid having OH group on it? What do you want to use it for?
Mar
4
accepted The effect of the start codon GTG on translation in E. coli
Mar
3
comment Does an annealing temp higher than primer's Tm contribute to primer dimer?
Hey what happened with your experiment?
Mar
2
reviewed Approve suggested edit on Taxonomy for plasmids
Feb
29
comment How long can E. coli stocks be stored at -20°C?
I might do that:) Can you email me (gvandova@stanford.edu) with some more details about it?
Feb
29
comment What is the least costly method to generate sequential amino acid deletions?
How big is your gene and your vector?
Feb
29
reviewed Approve suggested edit on How can I avoid digesting protein-bound DNA?
Feb
28
comment How long can E. coli stocks be stored at -20°C?
You can also start keeping transformed cells (with some drug resistance marker) at -20°C, and plate some of them every day and see how quickly they will lose the plasmid. You can do the same with untransformed competent cells.
Feb
28
revised PCR amplification and error propagation
added 44 characters in body
Feb
28
comment How long can E. coli stocks be stored at -20°C?
@shigeta I didn't know that proteases are active at -20°C (leonardo's answer). But it is worth the shot (at least short-term) if you don't store the cells too long.
Feb
28
comment How long can E. coli stocks be stored at -20°C?
Which enzymes are active at -20degrees?
Feb
27
revised PCR amplification and error propagation
added an exception and short explanation of the answer
Feb
27
reviewed Approve suggested edit on PCR amplification and error propagation
Feb
27
revised PCR amplification and error propagation
Make it easier to read