4,283 reputation
11039
bio website linkedin.com/profile/…
location Stanford, CA
age 28
visits member for 2 years, 4 months
seen 22 hours ago

Biochemistry PhD student at Stanford University. Interested in natural products engineering and synthetic biology.


Mar
14
comment Is there an enzyme for the transformation of the hydroxyl group?
Can you give us some background? What is your aromatic compound? Why do you want to avoid having OH group on it? What do you want to use it for?
Mar
4
accepted The effect of the start codon GTG on translation in E. coli
Mar
3
comment Does an annealing temp higher than primer's Tm contribute to primer dimer?
Hey what happened with your experiment?
Mar
2
reviewed Approve suggested edit on Taxonomy for plasmids
Feb
29
comment How long can E. coli stocks be stored at -20°C?
I might do that:) Can you email me (gvandova@stanford.edu) with some more details about it?
Feb
29
comment What is the least costly method to generate sequential amino acid deletions?
How big is your gene and your vector?
Feb
29
reviewed Approve suggested edit on How can I avoid digesting protein-bound DNA?
Feb
28
comment How long can E. coli stocks be stored at -20°C?
You can also start keeping transformed cells (with some drug resistance marker) at -20°C, and plate some of them every day and see how quickly they will lose the plasmid. You can do the same with untransformed competent cells.
Feb
28
revised PCR amplification and error propagation
added 44 characters in body
Feb
28
comment How long can E. coli stocks be stored at -20°C?
@shigeta I didn't know that proteases are active at -20°C (leonardo's answer). But it is worth the shot (at least short-term) if you don't store the cells too long.
Feb
28
comment How long can E. coli stocks be stored at -20°C?
Which enzymes are active at -20degrees?
Feb
27
revised PCR amplification and error propagation
added an exception and short explanation of the answer
Feb
27
reviewed Approve suggested edit on PCR amplification and error propagation
Feb
27
revised PCR amplification and error propagation
Make it easier to read
Feb
27
reviewed Approve suggested edit on How long can E. coli stocks be stored at -20°C?
Feb
27
answered PCR amplification and error propagation
Feb
27
comment How long can E. coli stocks be stored at -20°C?
When I said a freeze-thaw cycle, I meant when you take the frozen sample from the freezer, do something with it (it thaws), and then put it back in the freezer (it freezes).
Feb
27
answered How long can E. coli stocks be stored at -20°C?
Feb
23
comment Why do restriction enzymes tend to have an even number of bases in their recognition site?
@RoryM I'd thought so ;-)
Feb
23
comment Why do restriction enzymes tend to have an even number of bases in their recognition site?
@RoryM CAT TAC is not a palindrome. CAT ATG is a palindrome.