4,542 reputation
11046
bio website linkedin.com/profile/…
location Stanford, CA
age 28
visits member for 2 years, 10 months
seen Oct 19 at 20:26

Biochemistry PhD student at Stanford University. Interested in natural products engineering and synthetic biology.


Feb
29
reviewed Approve suggested edit on How can I avoid digesting protein-bound DNA?
Feb
28
comment How long can E. coli stocks be stored at -20°C?
You can also start keeping transformed cells (with some drug resistance marker) at -20°C, and plate some of them every day and see how quickly they will lose the plasmid. You can do the same with untransformed competent cells.
Feb
28
revised PCR amplification and error propagation
added 44 characters in body
Feb
28
comment How long can E. coli stocks be stored at -20°C?
@shigeta I didn't know that proteases are active at -20°C (leonardo's answer). But it is worth the shot (at least short-term) if you don't store the cells too long.
Feb
28
comment How long can E. coli stocks be stored at -20°C?
Which enzymes are active at -20degrees?
Feb
27
revised PCR amplification and error propagation
added an exception and short explanation of the answer
Feb
27
reviewed Approve suggested edit on PCR amplification and error propagation
Feb
27
revised PCR amplification and error propagation
Make it easier to read
Feb
27
reviewed Approve suggested edit on How long can E. coli stocks be stored at -20°C?
Feb
27
answered PCR amplification and error propagation
Feb
27
comment How long can E. coli stocks be stored at -20°C?
When I said a freeze-thaw cycle, I meant when you take the frozen sample from the freezer, do something with it (it thaws), and then put it back in the freezer (it freezes).
Feb
27
answered How long can E. coli stocks be stored at -20°C?
Feb
23
comment Why do restriction enzymes tend to have an even number of bases in their recognition site?
@RoryM I'd thought so ;-)
Feb
23
comment Why do restriction enzymes tend to have an even number of bases in their recognition site?
@RoryM CAT TAC is not a palindrome. CAT ATG is a palindrome.
Feb
23
comment Why do restriction enzymes tend to have an even number of bases in their recognition site?
-1 for justifying your answer by defining palindromes as sequences with even number of bases. EcoRII recognizes the palindrome CCWGG (W being A or T).
Feb
22
revised Which is more important for protein expression mRNA structure or codon optimization?
Replaced the urls with the titles of the references
Feb
22
answered Which is more important for protein expression mRNA structure or codon optimization?
Feb
22
comment Questions to ask to a panel of people that will be sequenced
I think that if 23andMe ask these questions, there is a strong reason for their selection. And if you pay the money for your genome to be sequenced, you would want to answer as many questions as they ask you.
Feb
22
comment Questions to ask to a panel of people that will be sequenced
Please people, don't talk about dominant/recessive traits! Best case scenario, there are some associations ;-) Besides, the 'linux gene' would have more environmental effect.
Feb
22
asked How crowded is the bacterial cell?