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Feb
2
comment Does an annealing temp higher than primer's Tm contribute to primer dimer?
You can also contact the authors of some the papers you are trying to reproduce and ask them about the specific conditions they used. You shouldn't spend much time troubleshooting this by yourself.
Feb
2
comment DNA synthesis companies: cost per base, turn-around time, codon-optimization algorithms
Thanks, I already looked at it, but yeah, it is really useful.
Feb
1
comment DNA synthesis companies: cost per base, turn-around time, codon-optimization algorithms
Thanks for your answer @Amy. So, you have experience with GenScript. I would like to ask you some specific questions. Would you mind emailing me so that I don't extend my comment into long discussion? My email is gvandova@stanford.edu
Feb
1
answered Does an annealing temp higher than primer's Tm contribute to primer dimer?
Feb
1
awarded  Enthusiast
Feb
1
comment Does an annealing temp higher than primer's Tm contribute to primer dimer?
What kind of polymerase are you using?
Jan
30
comment Where is the line between Anaerobic and Aerobic?
@JoeHobbit It might be that denitrifying bacteria have Krebs cycle, but I think that this still classifies them as anaerobs. I will do more research and will let you know.
Jan
30
comment DNA synthesis companies: cost per base, turn-around time, codon-optimization algorithms
These are good points. I would thing that the information about cost will be valid once you match it with the date on which the answers are posted. As for turn-around time, I came across a blog, where there was a plot of turn-around time vs length of DNA construct for several companies, so average values can be definitely taken into account. But mostly, I really would love to know about their codon-optimization algorithms but perhaps you are right and they are proprietary. Hopefully, the main ideas are not.
Jan
30
answered How do you knockout an E. coli gene without disrupting the rest of the gene cluster?
Jan
27
asked DNA synthesis companies: cost per base, turn-around time, codon-optimization algorithms
Jan
26
comment How are the gene sequences of individual sperm and egg cells “randomized”?
are you familiar with concepts like haploid, homozygos, alleles, chrossing over? I am trying to figure out how high scientific level my answer should be.
Jan
25
comment What is Peter's rule in neuroscience?
Your link is not working. Also, it would be nice if you give a short 'informal' description of it yourself.
Jan
25
awarded  Announcer
Jan
24
comment What is the criticality of the ribosome binding site relative to the start codon in prokaryotic translation?
Yeah, we will see where this site is going...I am also trying the RBS calculator :-)It would be nice if we chat more. Would you write me on gvandova@stanford.edu?
Jan
23
comment Where is the line between Anaerobic and Aerobic?
@jp89 All energy produced in the Krebs cycles comes from the electron transport chain, i. e. energy cannot be produced without oxygen.
Jan
23
revised Where is the line between Anaerobic and Aerobic?
added 1 characters in body
Jan
23
revised Where is the line between Anaerobic and Aerobic?
added 11 characters in body
Jan
23
comment Where is the line between Anaerobic and Aerobic?
I still don't think that denitrifying bacteria will utilize Krebs cycle. Why would they do it anyways if no energy whatsoever comes from it?
Jan
23
answered Where is the line between Anaerobic and Aerobic?
Jan
23
revised Where is the line between Anaerobic and Aerobic?
eddited the name of Krebs cycle