Gergana Vandova

3,698 reputation

1634

bio	website	linkedin.com/profile/…
	location	Palo Alto, CA
	age	27
visits	member for	1 year, 5 months
	seen	May 11 at 0:28
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Visiting scholar at Stanford Genome Technology Center. Interested in synthetic biology.

	3,698 reputation	bio	website	linkedin.com/profile/…	visits	member for	1 year, 5 months
1634 badges		location	Palo Alto, CA		seen	May 11 at 0:28

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Feb 26	comment	Effect of doubling volumes of PCR reagents But presumably, @Armatus used different thermocyclers and he entered the appropriate volume of the sample in the thermocycler options.
Feb 26	comment	Effect of doubling volumes of PCR reagents Could you attach a picture of the gel? Also, how many times was this experiment repeated? I have used different volumes many times with no difference in efficiency (but with Phusion polymerase)
Feb 7	comment	What is the difference between SOLiD, 454, and Illumina next-gen sequencing? @MattDMo Yeah, it was pasted from an essay/review which I wrote for next generation sequencing. I will add the references.
Dec 10	comment	Can compounds be predicted from Polyketide synthases gene clusters? Each module adds a two (not three) carbon extender unit to the growing polyketide chain. Pleas correct it in your text.
Dec 10	comment	Can compounds be predicted from Polyketide synthases gene clusters? I would love to talk to you about this subject after my final :-) Why are you interested?
Nov 11	comment	Recombinant protein fraction in E. coli I also had a feeling that the protein concentration should be more uniform, especially for the same protein (GFP in this case). I also imagined it would not be lower than 40%.....
Oct 15	comment	How are the boundaries of a gene determined? @ghchinoy Yes, that could be interesting, but I don't think it is more complicated than I already explained to Richard. You can of course add a few more "checks" the software can do, like the length of the ORF.
Oct 15	comment	How are the boundaries of a gene determined? @RichardSmith They are basically looking for start codons (ATG, GTG) that define the beginning of the open reading frame (ORF), and stop codons (TAG, TAA, TGA), that define the end of the ORF, and also checks whether the number of bases between the start codon and the stop codon is devisible by 3.
Sep 17	comment	Are human fetuses more likely to be male? @nico: Done, thanks.
Jul 3	comment	How would one describe the R-factor in crystallography? And what is the background of the person you would like to explain the R-factor concept to?
Jun 29	comment	T7 promoter leakiness Isn't it possible that a regular E. coli strain that does not have T7 polymerase, has other polymerases that can recognize the T7 promoter?
Jun 15	comment	Designing genes with DNAWorks: Maximum nonzero score? @bobthejoe I am trying to say that the design portion is not the bottleneck.
Jun 14	comment	Designing genes with DNAWorks: Maximum nonzero score? Can you give us more information on what you are trying to assemble (size, length of overlap, length of oligos) and how you plan to assemble it?
May 26	comment	Co-transformation of plasmids from the same incompatibility group Thanks for your answer. I actually co-transformed and I got twice as many colonies compared to a uni-transformation. I think there might me something wrong with the plates or the cells, but perhaps the co-transformation is more efficient. Which I still don't understand why would that be....
Mar 22	comment	How to clone and sequence a gene transcript of unknown sequence? Do you know the function of the gene? What is the purpose of your experiment?
Mar 14	comment	What is causing my problem with very low yields when isolating a 42kb yeast plasmid? What should be the OD of the culture? What is the volume of the culture?
Mar 14	comment	Which is more important for protein expression mRNA structure or codon optimization? I spent quite some time trying to answer the same question, so I couldn't help but share my knowledge!
Mar 14	comment	Is there an enzyme for the transformation of the hydroxyl group? Can you give us some background? What is your aromatic compound? Why do you want to avoid having OH group on it? What do you want to use it for?
Mar 3	comment	Does an annealing temp higher than primer's Tm contribute to primer dimer? Hey what happened with your experiment?
Feb 29	comment	How long can E. coli stocks be stored at -20°C? I might do that:) Can you email me (gvandova@stanford.edu) with some more details about it?