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Apr
28
comment de novo gene synthesis vs cDNA library creation from plant RNA: Pros & Cons
Isn't "collecting plant samples, cryopreservation, digestion, extraction etc." also expensive? Think about cost and time. How long would it take to isolate the DNA vs synthesize it?
Apr
26
comment What is the highest number of genes expressed under one T7 promoter?
@aaaaaa Not really, since I would like to avoid adding extra sequence to the coding region.
Apr
26
comment Why does pETDuet-1 only have one T7 terminator but two T7 promoters?
@WYSIWYG Added vector map. The genes are not overlapping.
Apr
26
comment Why does pETDuet-1 only have one T7 terminator but two T7 promoters?
@Jory But in that case why is there a second promoter?
Apr
25
comment Why does pETDuet-1 only have one T7 terminator but two T7 promoters?
Not sure it can. But waiting from reply from them as well.
Apr
17
comment Online multiple sequence alignment with constraints
I also don't understand your constraints. Also, why do you need constraints? Usually when you do MSA, you don't pose any constraints since you presumably don't know how the query sequences can be aligned. Also, I bet that if you include sequence "p" in your MSA, the alignment will be different and will include your constraints.
Apr
15
comment Can one talk about deacetylation of a promoter rather than associated histone?
I would say yes, it is probably acceptable, but I agree that the authors should have been more clear by stating that changing the acetylation state of the histones wrapping the promoter affects the expression of the gene from that promoter.
Apr
15
comment Can one talk about deacetylation of a promoter rather than associated histone?
The promoter sequence is wrapped by histones, which a modified (look at figure 3). "KLF11 binds distinct nuclear cofactor complexes that posttranslationally modify promoter histones to epigenetically alter the chromatin state, gene expression levels, and activity"
Apr
8
comment Is there any virus that contains both DNA and RNA in its genome?
@anongoodnurse. You are correct, these viruses contain only DNA as their genetic material. But they also contain this capsid protein found exclusively in RNA viruses. So technically, yes, it is a DNA virus. Evolutionary, it is probably a DNA:RNA hybrid virus.
Apr
8
comment Is there any virus that contains both DNA and RNA in its genome?
Here is the real scientific paper:biologydirect.biomedcentral.com/articles/10.1186/1745-6150-7-13
Apr
8
comment Is there any virus that contains both DNA and RNA in its genome?
RDHV virus. science.opposingviews.com/… First google search result.
Apr
8
comment What is the biological significance of k-mer counting?
These 3 applications are fundamental in biology. I gave short descriptions of each one. You can look them up if you want to know more.
Apr
7
comment Are there proteins that stabilize mRNA:RNA polymerase or mRNA:ribosome complex?
That's a very good (and interesting) question. Will let you know if I find the answer.
Apr
6
comment Are there proteins that stabilize mRNA:RNA polymerase or mRNA:ribosome complex?
I am not saying they exist 100%. I am just saying that it makes sense.
Apr
6
comment Are there proteins that stabilize mRNA:RNA polymerase or mRNA:ribosome complex?
A professor I talked to at a conference mentioned it, so I know they exist, I just don't know how they are called. And it definitely makes sense for them to exist, since these ORFs are so long.
Jul
29
comment E coli cotransformation efficiency
Thanks, Chris, but I was more wondering how high with efficiency (number of clones per ug DNA) you can get with co-transformation. For example for single electroporation the efficiency is around 1 × 10^10 cfu/μg so I would like to know what is the limit and whether it can be improved for co-transformation.
Jul
10
comment Efficiency of plasmid DNA isolation from frozen E. coli cell cultures
Thanks Chris. I tried it with 25ml cultures and I got great yields! I did not run them on a gel to check the quality of the DNA, but the sequencing results looked good!
Mar
14
comment Resources for interesting facts about biology
Well I think that jarlemag's answer is pretty good and important for the community. Too bad that others can't post other sources if they stumble upon them. I actually disagree that the answer will be opinion-based, as I would expect people to provide resources, not what they would consider to be "fun or important". If you have any suggestions how to change my question to reflect this, let me know.
Mar
12
comment Resources for interesting facts about biology
That's cool! Yeah, that's what I am talking about. And this is not just for me, but for the community. So whatever I don't find interesting, I am sure others will :) Your 'BioNumbers" link is not working.
Mar
5
comment How do mosquitoes maintain telomere length?
I can actually help you with that! Steve Artandi (Stanford University) is working on telomeres. You might be able to talk to one of his students... Email me at gvandova@stanford.edu if you are interested.