4,602 reputation
11247
bio website linkedin.com/profile/…
location Stanford, CA
age 28
visits member for 3 years
seen Nov 11 at 22:21

Biochemistry PhD student at Stanford University. Interested in natural products engineering and synthetic biology.


Jul
29
comment E coli cotransformation efficiency
Thanks, Chris, but I was more wondering how high with efficiency (number of clones per ug DNA) you can get with co-transformation. For example for single electroporation the efficiency is around 1 × 10^10 cfu/μg so I would like to know what is the limit and whether it can be improved for co-transformation.
Jul
10
comment Efficiency of plasmid DNA isolation from frozen E. coli cell cultures
Thanks Chris. I tried it with 25ml cultures and I got great yields! I did not run them on a gel to check the quality of the DNA, but the sequencing results looked good!
Mar
14
comment Resources for interesting facts about biology
Well I think that jarlemag's answer is pretty good and important for the community. Too bad that others can't post other sources if they stumble upon them. I actually disagree that the answer will be opinion-based, as I would expect people to provide resources, not what they would consider to be "fun or important". If you have any suggestions how to change my question to reflect this, let me know.
Mar
12
comment Resources for interesting facts about biology
That's cool! Yeah, that's what I am talking about. And this is not just for me, but for the community. So whatever I don't find interesting, I am sure others will :) Your 'BioNumbers" link is not working.
Mar
5
comment How do mosquitoes maintain telomere length?
I can actually help you with that! Steve Artandi (Stanford University) is working on telomeres. You might be able to talk to one of his students... Email me at gvandova@stanford.edu if you are interested.
Mar
5
comment How do mosquitoes maintain telomere length?
No I am not but I know the principle. You should just read this paper and try see whether you believe the interpretation of their data. Also just wait to see what other people might say about that.
Mar
5
comment How do mosquitoes maintain telomere length?
mm that's a good question. My first thought would be no, as eukaryotic cells are in general capable of recombination. I am not really familiar with mosquitoes, but would assume that applies for them as well. But again, it might be possible that there are telomere-specific recombination enzymes. Worth looking into.
Mar
5
comment Plasmid maintenance
Yeah, I think this is a good backup.
Mar
5
comment Using PSI-BLAST
Yes, each time you iterate a new PSSM is formed. You might want to use it if you would want to identify homologs that are undetectable by simple BLAST.
Mar
3
comment Does the MS2 RNA binding protein have any translational repression effects?
Oh I see now... Interesting idea. Worth exploring. Why don't you use Cas9?
Feb
25
comment What is the fastest way to build an alanine scanning library?
Are you still interested in the answer of this question?
Feb
25
comment Does the MS2 RNA binding protein have any translational repression effects?
Why don't you place the MS2L downstream of the ORF? What exactly is the experiment you want to do?
Feb
25
comment What are the effect of microtubule or microfilament inhibition on yeast expression profile?
Thanks for the answer. Could you summarize what was discovered? Which genes were downregulated/upregulated/unchanged? Thanks.
Feb
19
comment Sequence analysis software suites
My pleasure! Are you a benchling user?
Feb
19
comment Sequence analysis software suites
All of this you can do with benchling (see answer below). And as it is web based it is not operating system dependent. As far as I know Geneious and GeneDesigner don't work in Linux.
Feb
26
comment Effect of doubling volumes of PCR reagents
But presumably, @Armatus used different thermocyclers and he entered the appropriate volume of the sample in the thermocycler options.
Feb
26
comment Effect of doubling volumes of PCR reagents
Could you attach a picture of the gel? Also, how many times was this experiment repeated? I have used different volumes many times with no difference in efficiency (but with Phusion polymerase)
Feb
7
comment What is the difference between SOLiD, 454, and Illumina next-gen sequencing?
@MattDMo Yeah, it was pasted from an essay/review which I wrote for next generation sequencing. I will add the references.
Dec
10
comment Can compounds be predicted from Polyketide synthases gene clusters?
Each module adds a two (not three) carbon extender unit to the growing polyketide chain. Pleas correct it in your text.
Dec
10
comment Can compounds be predicted from Polyketide synthases gene clusters?
I would love to talk to you about this subject after my final :-) Why are you interested?