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bio website danriggins.blogspot.com
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visits member for 1 year, 7 months
seen Aug 21 at 1:38

Aug
7
accepted What's the recommended maximum passage number for mammalian cells (NCI-H441)?
Feb
3
comment What's the recommended maximum passage number for mammalian cells (NCI-H441)?
About 9 weeks. :/ I made a lot of dumb errors at the start.
Feb
3
comment What's the recommended maximum passage number for mammalian cells (NCI-H441)?
Yeah, unfortunately this was the first time I had done any mammalian tissue culturing, by the time I had grown enough to make a sufficiently large stock of frozen cells, I had already hit passage number 6. I was thinking I would draw from frozen stock each time my current line hits passage number 10. Does that seem reasonable or should I buy new cells? We sourced our current cells directly from the American Type Culture Collection.
Jan
30
asked What's the recommended maximum passage number for mammalian cells (NCI-H441)?
Feb
1
comment What accounts for size variation in breeds of dogs?
Shigeta did a great job summarizing what we know about genotypes and dog size variations, does anyone know whether there are wild species that also very so dramatically in size?
Feb
1
awarded  Scholar
Feb
1
awarded  Supporter
Feb
1
accepted What accounts for size variation in breeds of dogs?
Jan
31
awarded  Student
Jan
31
asked What accounts for size variation in breeds of dogs?
Jan
31
answered What makes E. coli yellowish?
Jan
31
awarded  Editor
Jan
31
revised Why am I getting low transformation efficiency with DB3.1 E.coli cells?
added 33 characters in body
Jan
31
answered Why am I getting low transformation efficiency with DB3.1 E.coli cells?
Jan
30
answered How does Serotonergic (5-HT2A mediated) Psychedelia work?
Jan
30
awarded  Teacher
Jan
30
answered Are all known germs benign in small enough quantities? i.e. Why do we wash our hands?