129 reputation
6
bio website
location Heidelberg, Germany
age
visits member for 1 year, 10 months
seen Dec 18 at 22:39

Dec
18
awarded  Excavator
Dec
18
revised RNA seq and using of Poly(A) or non-Poly(A) based amplification of RNA
removed proxy from link
Dec
18
suggested approved edit on RNA seq and using of Poly(A) or non-Poly(A) based amplification of RNA
Dec
17
comment How do I get recombinant proteins into the nucleus of mammal cells?
Thanks for the general tip. In my case I have seen people putting stuff (including NLS) to both ends of the protein. Here I am really more concerned about transport efficiency.
Dec
17
asked How do I get recombinant proteins into the nucleus of mammal cells?
Aug
6
accepted Why does the pET- expression vector contain a LacI gene additionally to the one in the genome?
Aug
4
comment Why does the pET- expression vector contain a LacI gene additionally to the one in the genome?
Thanks for you comment @Chris. Are there strains that have the T7 RNA polymerase but do not have lacI? Or are those then just infected with T7 phage to express the protein? Could you find a reference for your statement and post it as an answer?
Aug
4
asked Why does the pET- expression vector contain a LacI gene additionally to the one in the genome?
Mar
18
revised What is the full name of E. G. Gray?
deleted 3 characters in body
Feb
6
comment What's the use of RNA editing in kinetoplast?
Thanks! I kinda agree on that last point through I don't think that this really challenges the neutral hypothesis ("see we have 850 neutral gRNA developed by chance. Now if a mutation in a gene occurs that can be rescued by one of those gRNA this change will also be neutral but will lead to a fixation of that gRNA"). Is Neutrality even the basis of diversity and thus adjustability?(and thus not neutral, hah)
Feb
2
accepted What's the use of RNA editing in kinetoplast?
Feb
2
comment What's the use of RNA editing in kinetoplast?
Haha, I read that last quote just the second before I read your answer. I find this answer much more satisfying than the neutral model proposed in 10.1016/0168-9525(93)90011-6. I like how you point out the different oxygen concentrations[source would be nice] as one would usually think of the mitochondrial genes as almost perfect and thus less variable. However, some (healthy?) doubt is left...
Feb
2
asked What's the use of RNA editing in kinetoplast?
Sep
26
awarded  Student
Sep
25
comment What is the full name of E. G. Gray?
It's about his bio, isn't? * ironic smile * I shared your doubts, but I think this question will be useful especially for people using this SE site. Though I have to admit that main reason I asked it here instead of a more general site like Yahoo answers, is that I knew the people here were most likely to answer this question correctly. And Indeed it was correctly and comprehensively answered within minutes by @jonsca. Thank You very much therefor!
Sep
25
awarded  Scholar
Sep
25
accepted What is the full name of E. G. Gray?
Sep
25
comment How do I measure bacterial growth in agar dishes (either by cell mass or by cell count)?
I don't understand the effect of not "always ensuring you inoculate the plates with the same amount of inoculum."
Sep
25
asked What is the full name of E. G. Gray?
Sep
4
comment What is Peter's rule in neuroscience?
"taking into account the detailed morphometrics", i.e. looking on an EM image? :P see another exception