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Aug
16
revised Visualizing secondary RNA structure binding to target
added 13 characters in body
Aug
16
asked Visualizing secondary RNA structure binding to target
Aug
16
reviewed Approve Why do plasmids replicate on their own?
Jul
31
asked Do the phage repressors CI and Mnt exhibit crosstalk?
Jul
30
comment Why isn't there a standard unit of promoter strength?
Not all genes need to be expressed in their "proper" context. Think of the Tet and lambda pR promoters being expressed in E coli (or really synthetic biology as a whole). If the purpose is exploiting or engineering and not discovering novel protein functions at a particular developmental stage, these promoters strengths are extremely relevent.
Jul
30
comment Why isn't there a standard unit of promoter strength?
@Amory, this is exactly why a characterized number would be useful to define promoter strength. You would be able to quantify a particular promoter's strength with or without activators/repressors, as well as in relation to other promoters with their respective regulatory elements.
Jul
30
comment Why isn't there a standard unit of promoter strength?
I do realize that transcription is very context-dependent, but disagree about its importance. Even if strictly used in characterized in vitro systems, a number associated with a promoter is very useful, and can be extrapolated to its general abilities for transcription in vivo.
Jul
30
revised Why isn't there a standard unit of promoter strength?
added 75 characters in body
Jul
30
reviewed Approve sex tag wiki excerpt
Jul
30
reviewed Approve vertebrates tag wiki
Jul
30
reviewed Approve sex tag wiki
Jul
30
reviewed Approve snp tag wiki
Jul
30
reviewed Approve vertebrates tag wiki excerpt
Jul
30
asked Why isn't there a standard unit of promoter strength?
Jul
24
asked Is there are practical lower limit to gene length in E. coli?
Jul
19
comment Will eukaryotic RNA fold in the same way in prokaryotes?
@MadScientist, in my specific case I was more interested in secondary structure, but an answer addressing tertiary would be ideal
Jul
18
asked Will eukaryotic RNA fold in the same way in prokaryotes?
Jul
18
comment Can two hammerhead ribozymes simultaneously cleave each other?
@WYSIWYG, I plan on having longer flanking arms and 100% complementarity between the ribozymes, and less so for their target.
Jul
17
revised Can two hammerhead ribozymes simultaneously cleave each other?
added 7 characters in body
Jul
16
asked Can two hammerhead ribozymes simultaneously cleave each other?