3,441 reputation
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bio website gravatar.com/lancelafontaine
location Montreal, Canada
age 22
visits member for 2 years, 6 months
seen Jul 21 at 23:08

Hey there, my name is Lance Lafontaine.

I am a student at Concordia University in software engineering, with a previous degree in molecular biology. I love all things tech, science and startups.

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Jul
6
comment Convert gene name to uniprot ID
Can you explain what these links do? Just posting links as an answer is bad practice: meta.stackexchange.com/questions/8231/…
Dec
17
comment DNA degradation rate
@Chris, exactly. If you treat your DNA well, it would treat you well (experimentally, that is). :)
Dec
17
comment DNA degradation rate
Not that common, but just think about how it only takes one enzyme to destroy your entire sample. A "source" of nucleases can be from your fingertips too, which is why its very important to wear gloves when dealing with DNA directly.
Oct
22
comment Does the Petri dish lid orientation on workbench affect aseptic technique?
Plating = Spreading.
Oct
18
comment Are there known 3- or 4-nucleotide RNA linkers with no RE sites?
Question edited, thanks for the suggestion.
Oct
18
comment Bioinformatics tool for “pairwise alignment” of complementary sequences?
@terdon On second thought, would doing a regular pairwise alignment with the reverse-complement of only of the sequences yield the same result?
Oct
17
comment Bioinformatics tool for “pairwise alignment” of complementary sequences?
Sorry for the confusion: looking for regions of high complementarity. Will edit question in a moment.
Jul
30
comment Why isn't there a standard unit of promoter strength?
Not all genes need to be expressed in their "proper" context. Think of the Tet and lambda pR promoters being expressed in E coli (or really synthetic biology as a whole). If the purpose is exploiting or engineering and not discovering novel protein functions at a particular developmental stage, these promoters strengths are extremely relevent.
Jul
30
comment Why isn't there a standard unit of promoter strength?
@Amory, this is exactly why a characterized number would be useful to define promoter strength. You would be able to quantify a particular promoter's strength with or without activators/repressors, as well as in relation to other promoters with their respective regulatory elements.
Jul
30
comment Why isn't there a standard unit of promoter strength?
I do realize that transcription is very context-dependent, but disagree about its importance. Even if strictly used in characterized in vitro systems, a number associated with a promoter is very useful, and can be extrapolated to its general abilities for transcription in vivo.
Jul
19
comment Will eukaryotic RNA fold in the same way in prokaryotes?
@MadScientist, in my specific case I was more interested in secondary structure, but an answer addressing tertiary would be ideal
Jul
18
comment Can two hammerhead ribozymes simultaneously cleave each other?
@WYSIWYG, I plan on having longer flanking arms and 100% complementarity between the ribozymes, and less so for their target.
Jun
12
comment Breaking of fats
Not everything in biology is black or white (in fact, almost everything is gray). A combination of these factors and others play a role in the observation.
May
31
comment Is there a practical upper limit to amount of nucleotides or genes in a transformed plasmid?
A BAC might work. Thank you for the advice. You should turn your comments into an answer.
May
31
comment Is there a practical upper limit to amount of nucleotides or genes in a transformed plasmid?
I'll most likely be working with E.coli. Putting aside differences in transformation, would there be any reason to suspect differences in functional expression of each gene? (If there is, I've never heard of it. It would seem like genes should get expressed equally in no matter what lengths of nucleotide).
May
16
comment Are humans more aggressive during a full moon?
This is more fit for Skeptics and has already been asked there. skeptics.stackexchange.com/questions/948/…
Apr
15
comment What would it take to recognize a deer by its photo?
It seems as though one of your only options is deer-adapted facial recognition software (as silly as it might sound) which is definitely not within the scope of biology.
Apr
15
comment What would it take to recognize a deer by its photo?
I'm not so sure about software... but it would help to know what particular trait you are examining, exactly. You can quantify fur color by spectrometry, for example.
Apr
15
comment How is the appropriately-stratified gut microbiome acquired in organisms performing horizontal transmission?
Relevent: ncbi.nlm.nih.gov/pubmed/15831718 and sciencemag.org/content/312/5778/1355.short
Apr
15
comment Are there natural selection experiments that quantitatively agree with theoretical modeling?
If this is possible, I'd be particularly interested in finding out what variables they took into account in their calculations.