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seen Oct 13 at 21:13

PhD student in chemical engineering


Apr
6
comment Are cells really the basic unit of all life?
Now that I've actually sat down and thought about it, this definition does fit; +1. H2O can't be life since it doesn't add any variation to itself.
Apr
6
comment Are cells really the basic unit of all life?
@nico, CHM, Preece. You guys better be satisfied.
Apr
6
revised Are cells really the basic unit of all life?
Added stupid references for everyone's satisfaction.
Apr
6
revised How do SDS-PAGE gels differ in a Bis-Tris system vs. a Tris-Glycine system?
added 63 characters in body
Apr
6
revised How do Proteins migrate in MES vs. MOPS
added 77 characters in body
Apr
5
comment Are cells really the basic unit of all life?
@Preece Gerry Joyce.
Apr
5
asked How do Proteins migrate in MES vs. MOPS
Apr
5
asked How do SDS-PAGE gels differ in a Bis-Tris system vs. a Tris-Glycine system?
Apr
5
comment Are cells really the basic unit of all life?
@CHM intriguing. I guess we have to start considering the battle against entropy.
Apr
5
comment Are cells really the basic unit of all life?
@nico, I think I have pointed out that viruses do not qualify under the above definition. Likewise as mentioned in biology.stackexchange.com/a/96/389, prions are dependent on host factors. Citations will come after I finish my experiments.
Apr
5
answered Are cells really the basic unit of all life?
Apr
4
comment Are cells really the basic unit of all life?
Well at the moment, there isn't a question related to what is the definition of life. Meanwhile, I think that most evolution of life specialist will say that no, cells are not the basic unit.
Apr
3
accepted Why does the T7 RNA Polymerase require a reducing environment ie. DTT
Apr
3
comment Ideal low-protein binding membranes
@CHM Grad student. I do this for research and the greater good of mankind.
Apr
2
comment How are there alternative initiation codons?
Leave it to Marilyn to clarify what everyone else jumbles. I'm seeing some suggestions that there are novel tRNAs with CAC/CAA anticodons. We'll get to the bottom of this.
Apr
2
revised How are there alternative initiation codons?
Improved grammar and additional tags.
Apr
2
asked How are there alternative initiation codons?
Mar
30
comment Alternatives to TBE buffer for denaturing polyacrylamide gel electrophoresis of DNA and RNA?
@leonardo, I've always heard that its the other way. CE is run at a high temperature to improve resolution. This is worth a question...
Mar
29
comment Alternatives to TBE buffer for denaturing polyacrylamide gel electrophoresis of DNA and RNA?
Not related to your question but what voltage are you running your gels? It seems as if your having all sorts of issues with over-heating.
Mar
29
comment For how long and how cold should I perform ethanol/isopropanol precipitations of RNA or DNA?
I think the folklore is influenced by how much nucleic acid you are working with. As @Daniel Standage suggests, some conditions wouldn't be influenced by anything.