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seen Jun 7 at 0:51

PhD student in chemical engineering


Apr
3
accepted Why does the T7 RNA Polymerase require a reducing environment ie. DTT
Apr
3
comment Ideal low-protein binding membranes
@CHM Grad student. I do this for research and the greater good of mankind.
Apr
2
comment How are there alternative initiation codons?
Leave it to Marilyn to clarify what everyone else jumbles. I'm seeing some suggestions that there are novel tRNAs with CAC/CAA anticodons. We'll get to the bottom of this.
Apr
2
revised How are there alternative initiation codons?
Improved grammar and additional tags.
Apr
2
asked How are there alternative initiation codons?
Mar
30
comment Alternatives to TBE buffer for denaturing polyacrylamide gel electrophoresis of DNA and RNA?
@leonardo, I've always heard that its the other way. CE is run at a high temperature to improve resolution. This is worth a question...
Mar
29
comment Alternatives to TBE buffer for denaturing polyacrylamide gel electrophoresis of DNA and RNA?
Not related to your question but what voltage are you running your gels? It seems as if your having all sorts of issues with over-heating.
Mar
29
comment For how long and how cold should I perform ethanol/isopropanol precipitations of RNA or DNA?
I think the folklore is influenced by how much nucleic acid you are working with. As @Daniel Standage suggests, some conditions wouldn't be influenced by anything.
Mar
28
comment Why does the T7 RNA Polymerase require a reducing environment ie. DTT
How strange. I'm looking at the crystal structure (1QLN) and there aren't a lot of cysteines near the active site. Will have to take a look at those papers.
Mar
27
comment Why does Penicillin only affect bacterial cell walls
I don't think that this is necessarily correct. The beta-lactams doesn't inhibit the synthesis but rather the reorganization of the peptidoglycans. The rigidity of the now static wall then prevents cell division.
Mar
27
asked Why does the T7 RNA Polymerase require a reducing environment ie. DTT
Mar
27
awarded  Benefactor
Mar
27
awarded  Enthusiast
Mar
27
revised Why is SOC medium recommended for transformations?
deleted 8 characters in body
Mar
27
revised What causes a 'stuffy' or 'runny' nose when you have a cold?
edited tags
Mar
26
revised Why is SOC medium recommended for transformations?
Added some relevant links.
Mar
26
answered Why is SOC medium recommended for transformations?
Mar
25
revised Why is the 3'UTR region highly methylated in most of the human genes?
added a tag
Mar
24
accepted How long does it take for E. coli to shift feedstocks?
Mar
23
comment Can methylation from DNA get copied to RNA during transcription?
@Garima Kushwaha. Does this count? ncbi.nlm.nih.gov/pmc/articles/PMC2836557