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visits member for 2 years, 2 months
seen Apr 12 at 23:27

PhD student in chemical engineering


Mar
29
comment Alternatives to TBE buffer for denaturing polyacrylamide gel electrophoresis of DNA and RNA?
Not related to your question but what voltage are you running your gels? It seems as if your having all sorts of issues with over-heating.
Mar
29
comment For how long and how cold should I perform ethanol/isopropanol precipitations of RNA or DNA?
I think the folklore is influenced by how much nucleic acid you are working with. As @Daniel Standage suggests, some conditions wouldn't be influenced by anything.
Mar
28
comment Why does the T7 RNA Polymerase require a reducing environment ie. DTT
How strange. I'm looking at the crystal structure (1QLN) and there aren't a lot of cysteines near the active site. Will have to take a look at those papers.
Mar
27
comment Why does Penicillin only affect bacterial cell walls
I don't think that this is necessarily correct. The beta-lactams doesn't inhibit the synthesis but rather the reorganization of the peptidoglycans. The rigidity of the now static wall then prevents cell division.
Mar
27
asked Why does the T7 RNA Polymerase require a reducing environment ie. DTT
Mar
27
awarded  Benefactor
Mar
27
awarded  Enthusiast
Mar
27
revised Why is SOC medium recommended for transformations?
deleted 8 characters in body
Mar
27
revised What causes a 'stuffy' or 'runny' nose when you have a cold?
edited tags
Mar
26
revised Why is SOC medium recommended for transformations?
Added some relevant links.
Mar
26
answered Why is SOC medium recommended for transformations?
Mar
25
revised Why is the 3'UTR region highly methylated in most of the human genes?
added a tag
Mar
24
accepted How long does it take for E. coli to shift feedstocks?
Mar
23
comment Can methylation from DNA get copied to RNA during transcription?
@Garima Kushwaha. Does this count? ncbi.nlm.nih.gov/pmc/articles/PMC2836557
Mar
22
comment How to clone and sequence a gene transcript of unknown sequence?
Having the hormone sequence does help a bit. While not entirely unbiased, you could try to enrich your sequence by amplifying from a conserved region.
Mar
21
asked Does the MS2 RNA binding protein have any translational repression effects?
Mar
21
asked What is the distance between the 3' 18s rRNA (the Kozak consensus sequence) and the A-site of eukaryotic ribosomes during protein translation?
Mar
21
asked How does a cell know its size?
Mar
21
awarded  Promoter
Mar
21
asked Providing small molecules to cells on a filter plate