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Apr
10
answered Neutralizing TCA washes
Apr
10
comment RNA or ribosome, which one moves during translation?
@Shigeta. I love the argument but conservation of momentum may not hold in these low-reynolds number systems.
Apr
10
comment RNA or ribosome, which one moves during translation?
I think any anyone who has seen relativity understands Nico's point. As for the paper, Warner et al. fix the ribosomes onto the surface. It's an artificial surface that forces the mRNA to be threaded through the polyribosomes. One can't make a conclusion from this study.
Apr
10
asked How straightforward is in vitro compartmentalization?
Apr
9
asked Neutralizing TCA washes
Apr
7
comment Quantum mechanics in biology
Don't forget about deuteriating drugs!
Apr
7
revised When collecting cell lysates for a Western blot, how do I induce di-sulfide bonds?
improved summary. Reeditted for the OP's question.
Apr
7
accepted How long does it take to stain cells?
Apr
6
asked How long does it take to stain cells?
Apr
6
answered When collecting cell lysates for a Western blot, how do I induce di-sulfide bonds?
Apr
6
asked What temperature should mammalian B-Cells be stored at outside of the incubator?
Apr
6
comment How do Proteins migrate in MES vs. MOPS
@leonardo. I feel that you're correct. Now that I'm looking at the chemical structure, I'm surprised that the single carbon difference creates that much difference in the pKa. However, if one is running a gel at the suggested pH for the buffers, the charge ratio should be the same.
Apr
6
revised Are cells really the basic unit of all life?
added 55 characters in body
Apr
6
comment Are cells really the basic unit of all life?
Now that I've actually sat down and thought about it, this definition does fit; +1. H2O can't be life since it doesn't add any variation to itself.
Apr
6
comment Are cells really the basic unit of all life?
@nico, CHM, Preece. You guys better be satisfied.
Apr
6
revised Are cells really the basic unit of all life?
Added stupid references for everyone's satisfaction.
Apr
6
revised How do SDS-PAGE gels differ in a Bis-Tris system vs. a Tris-Glycine system?
added 63 characters in body
Apr
6
revised How do Proteins migrate in MES vs. MOPS
added 77 characters in body
Apr
5
comment Are cells really the basic unit of all life?
@Preece Gerry Joyce.
Apr
5
asked How do Proteins migrate in MES vs. MOPS