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| visits | member for | 1 year, 4 months |
| seen | May 16 at 17:53 | |
| stats | profile views | 56 |
PhD student in chemical engineering
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Feb 27 |
revised |
Which cryogenic vials and caps are ideal for storing glycerol stocks? edited tags |
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Feb 27 |
comment |
Why do restriction enzymes tend to have an even number of bases in their recognition site? I would guess that the followup question is why is this the case? |
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Feb 27 |
asked | Which cryogenic vials and caps are ideal for storing glycerol stocks? |
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Feb 27 |
answered | Why does RNA adopt an A-form helix? |
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Feb 27 |
comment |
Determining potential protease sites within a recombinant protein Good answer that is relevant to the problem but not relevant to the question. |
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Feb 27 |
awarded | Citizen Patrol |
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Feb 25 |
asked | What are the most important differences between HSP70 and HSP90? |
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Feb 24 |
comment |
Are single-celled organisms capable of learning? This is an interesting point. A big issue with the toggle switch is that the network topology from Gardner's paper is rarely if ever seen in biology. However, there remains plenty of examples where hysteresis may occur in naturally relevant systems. |
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Feb 24 |
comment |
Determining potential protease sites within a recombinant protein I am using an E. coli expression strain but not BL21. Probably worth a try. I have tried using protease inhibitors and those haven't worked. |
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Feb 24 |
asked | Determining potential protease sites within a recombinant protein |
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Feb 24 |
comment |
What is the tRNA gene copy variation between different yeast strains? I would reask this question on BioStar. They may have better ideas than we mere experimentalists. |
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Feb 24 |
comment |
What is the tRNA gene copy variation between different yeast strains? It sounds like you should try a De Novo assembly. That might be tricky since you don't have any paired-end data but that might be able to discover any novel contigs. |
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Feb 24 |
comment |
How would one calculate the availability of nucleotides to an enzyme? The second question on DNA proofreading is probably worth an entire question by itself. You might as well ask it and get more reputation ;) |
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Feb 24 |
comment |
Can DNA produce ribozyme-like structures? In that regard, you should really cite Ned Seeman's work. The DNA in the box and the DNA origami work better reflect the conformational flexibility of higher ordered DNA structure. I would believe that the alternative discussion in biology.stackexchange.com/questions/287/… better describes the limitations of DNA in achieving these non-canonical structures. |
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Feb 22 |
asked | Which is more important for protein expression mRNA structure or codon optimization? |
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Feb 22 |
comment |
Can DNA produce ribozyme-like structures? This isn't a ribozyme at all. |
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Feb 22 |
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What is the tRNA gene copy variation between different yeast strains? What type of sequencing data do you have? Are we talking about RNA-seq or DNA? |
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Feb 22 |
awarded | Organizer |
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Feb 22 |
revised |
How crowded is the bacterial cell? edited tags |
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Feb 22 |
answered | How crowded is the bacterial cell? |
