meta user
network profile
| bio | website | |
|---|---|---|
| location | ||
| age | ||
| visits | member for | 1 year, 4 months |
| seen | May 16 at 17:53 | |
| stats | profile views | 56 |
PhD student in chemical engineering
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Feb 22 |
comment |
X chromosome “weight”? Is that difference due to the weight or rather the metabolism of the sperm due to having a different sized genome? |
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Feb 22 |
asked | Can a cell culture insert plate be used with a vacuum manifold? |
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Feb 18 |
asked | How often can you reuse coomassie stain? |
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Feb 17 |
comment |
What is the origin of “melting” in molecular genetics? From a statistical thermodynamics standpoint, DNA denaturation and renaturation is certainly a phase transition. The question is going from which phase to which? I would argue that the description of a rigid polymer going to a freed fluid polymer sounds like a solid to liquid transition to me. |
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Feb 17 |
answered | What is the tRNA gene copy variation between different yeast strains? |
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Feb 15 |
answered | Can protein structure be determined by X-Ray Diffraction in a single image? |
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Feb 15 |
awarded | Commentator |
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Feb 15 |
comment |
Can protein structure be determined by X-Ray Diffraction in a single image? CryoEM also doesn't use any X-ray beams either. |
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Feb 3 |
comment |
Why are restriction enzymes not frozen? It does get the point across. |
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Feb 3 |
awarded | Analytical |
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Feb 3 |
comment |
Is it the case that all changes in phenotype during life are not inheritable? That last paragraph is completely unnecessary. Anyone who knows what is going on would know this. |
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Feb 3 |
comment |
How does water buffer a sudden drop in temperature? Biologists should still know about statistical thermodynamics. |
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Feb 3 |
comment |
How do I clean phenol contaminated RNA without losing any of the sample? @jp89, From what I understand the IPA and Ethanol cleans only effectively take care of the salts. The Phenol Choloroform extraction does a much better job with the removal of proteins. Possibly you could take some of the interphase and clean again. The phenol will affect your columns and affect your yields. |
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Feb 1 |
awarded | Scholar |
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Feb 1 |
accepted | How do you knockout an E. coli gene without disrupting the rest of the gene cluster? |
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Jan 30 |
revised |
How do you knockout an E. coli gene without disrupting the rest of the gene cluster? edited tags |
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Jan 30 |
awarded | Student |
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Jan 30 |
asked | How do you knockout an E. coli gene without disrupting the rest of the gene cluster? |
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Jan 29 |
awarded | Enlightened |
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Jan 29 |
awarded | Nice Answer |
