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seen Apr 12 at 23:27

PhD student in chemical engineering


Feb
28
comment How strong is spider silk?
I hate to admit that wikipedia does pretty much answer this question. Followup question. What does it mean?
Feb
28
revised Ideal low-protein binding membranes
more details included
Feb
28
asked Ideal low-protein binding membranes
Feb
28
asked Is bootstrapping an acceptable way to determine standard error for binding constants?
Feb
28
asked How does one calculate the resolution of a crystal structure?
Feb
28
asked How strong is spider silk?
Feb
28
comment Determining potential protease sites within a recombinant protein
I should add that I've been using cell-free protein expression. However, the question at hand is completely independent of the expression system used.
Feb
28
revised What is the molecular mechanism of cystine bond formation?
changed the focus of the question.
Feb
28
awarded  Quorum
Feb
28
comment Determining potential protease sites within a recombinant protein
+1. Tried using the cOmplete tablets and still seeing problems. Do you have a good idea what's in the mix?
Feb
28
revised Why does cyanide inhibit CuZnSOD, but not MnSOD or FeSOD?
edited tags
Feb
28
comment How would one calculate the availability of nucleotides to an enzyme?
Wouldn't this be a mass-transport related question? I think that transport rate/flux of nucleotides can be described entirely by the diffusion coefficient given the cellular environment.
Feb
28
comment Determining potential protease sites within a recombinant protein
I have tried lower temperature expression at 25C but still see issues. I could go lower to 10C.
Feb
28
comment Does current evidence support the use of resveratrol as an anti-ageing drug?
I'm hesitant to upvote this answer. Much of the discussion on Resveratrol and proof of concepts of its pharmacodynamics don't come from Das's lab.
Feb
27
comment Which cryogenic vials and caps are ideal for storing glycerol stocks?
Hence the question. Do companies just charge us more to make us think that they are "necessary"?
Feb
27
comment What is the molecular mechanism of cystine bond formation?
I hope that biochemistry is on topic. Most reactive environments are actually reductive. An oxidative environment (via a periplasm or a chaperone) is important for cystine bond formation. I'm just curious about how those conditions are regulated. I hope that is clear.
Feb
27
asked What is the molecular mechanism of cystine bond formation?
Feb
27
revised Which cryogenic vials and caps are ideal for storing glycerol stocks?
edited tags
Feb
27
comment Why do restriction enzymes tend to have an even number of bases in their recognition site?
I would guess that the followup question is why is this the case?
Feb
27
asked Which cryogenic vials and caps are ideal for storing glycerol stocks?