4,402 reputation
1442
bio website
location
age
visits member for 2 years, 9 months
seen Oct 13 at 21:13

PhD student in chemical engineering


Mar
9
accepted What type of CCD system is required to take photos of luciferase
Mar
8
answered Can methylation from DNA get copied to RNA during transcription?
Mar
8
revised What is the difference between HPLC and FPLC and why is FPLC preferable for protein purification?
edited tags
Mar
7
comment What type of CCD system is required to take photos of luciferase
That I have done photo.stackexchange.com/q/20976/8852
Mar
7
asked What type of CCD system is required to take photos of luciferase
Mar
6
comment Does arterial blood always flow away from the heart?
Despite my contradicting conclusion, I fully endorse this answer. The answer entirely depends on how you calculate velocity. Is one looking at streamlines or bulk effects? If bulk, then the average flow should be going away as explained by @leonardo.
Mar
5
answered Does arterial blood always flow away from the heart?
Mar
5
comment How fast is the blood flow in various vessels?
You will have to account for mass balance using the Navier-Stokes equation which accounts for the cross-sectional area of the vessels.
Mar
4
comment Is bootstrapping an acceptable way to determine standard error for binding constants?
What is the definition of independent in this case? Does running a dilution series maintain independence? Now I'm even more curious how legitimate this is.
Mar
2
comment Is there a binding affinity metric for interactions not in equilibria?
Your basing your argument on psuedosteady state criteria and a constant concentration of your peptides and proteins. k_on and k_off are exclusively kinetic based terms that can be extended to thermodynamic terms when in equilibria which you're not in.
Mar
1
answered Is there a binding affinity metric for interactions not in equilibria?
Feb
29
comment What is the least costly method to generate sequential amino acid deletions?
Also see openwetware.org/wiki/Preparing_chemically_competent_cells
Feb
29
comment What is the least costly method to generate sequential amino acid deletions?
Probably deserves its own question. The answer is yes. I don't know how many passages E. coli can go through before they lose their chemical competency. One of these kits can make a couple hundred of shots. zymoresearch.com/e-coli/transformation-kits-accessories/…
Feb
29
revised What is the least costly method to generate sequential amino acid deletions?
added 192 characters in body
Feb
29
comment What is the least costly method to generate sequential amino acid deletions?
I may be biased but there is an entire MATLAB toolkit developed for this exact purpose for highthroughput alanine scanning using overlap extension. The nice benefit is that your costs gets reduced to only 1 oligo rather than 2 oligo per mutant. simtk.org/home/na_thermo
Feb
29
comment What is the least costly method to generate sequential amino acid deletions?
I've always made my own competent cells (DH5alpha cells which is why I don't know the costs). I would assume that you would want to keep your vector in an expression strain so if you want to skip a step, I would transform straight into BL21.
Feb
29
comment What is the least costly method to generate sequential amino acid deletions?
If we're thinking about this problem from the context of traditional biochemistry, I would just have nothing. That is if your gene is XABCDEFGHY, you now have XY. You still have the context of the rest of the "gene" to observe. It should be a nice negative control.
Feb
29
answered What is the least costly method to generate sequential amino acid deletions?
Feb
28
comment How strong is spider silk?
I hate to admit that wikipedia does pretty much answer this question. Followup question. What does it mean?
Feb
28
revised Ideal low-protein binding membranes
more details included