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seen Aug 12 at 21:34

PhD student in chemical engineering


Jun
7
comment Removing bacterial contaminants from resin
I am an engineer by training. The default option is always to fix it yourself. Usually with duct tape.
Jun
6
comment Removing bacterial contaminants from resin
@Shigeta, alas it wasn't my column. I guess it's time to buy some new resin.
Jun
1
comment Removing bacterial contaminants from resin
I'm going to try the high salt. That's a good suggestion. The reverse flow probably won't work since I've repacked the column a few times by now
Jun
1
asked Removing bacterial contaminants from resin
May
30
awarded  Popular Question
May
28
comment cell uptake prediction
cLogP? I would imagine that's a fast dirty way to get an estimate. en.wikipedia.org/wiki/Partition_coefficient
May
10
awarded  Popular Question
Apr
17
comment Are there any examples of sudden leaps in evolution?
I believe that the technical term is a "Hard Sweep".
Apr
10
comment What is the translation termination efficiency in mammalian cells?
From what I understand the eRFs are not perfect but they still work better than RF1 and RF2 which allow for a non trivial amount of readthrough.
Apr
10
comment What biochemical molecule viewer allows for changes in amino acids and resulting tertiary structure?
Pymol should have that capability... try Gromacs
Apr
9
comment What are the biological mechanisms behind the increase in cancer risk and alcohol consumption?
Asian Glow is linked with cancer. Actually tackling alchol related cancer is a fairly hot topic these days.
Apr
9
comment What are the biological mechanisms behind the increase in cancer risk and alcohol consumption?
I agree damaged is caused by acetaldehyde (also the cause of asian glow) but I believe that your selected enzyme is incorrect.
Apr
9
reviewed Approve suggested edit on How do the variable portions of antibody genes look in cells which don't produce antibodies?
Apr
9
reviewed Approve suggested edit on antibiotics tag wiki excerpt
Apr
9
comment How do you store membrane proteins?
@Suvidu, I would counter that if the purification protocol is a pain, it's a valuable investment to be able to scaleup effectively.
Apr
6
asked How do you store membrane proteins?
Apr
3
comment How prevalent is Taq polymerase in adding 3' A overhangs to the PCR product?
From what I understand, the A' overhang does eventually fall off. However, TA cloning is a much easier route if you have a TA vector.
Apr
2
revised How does Serotonergic (5-HT2A mediated) Psychedelia work?
edited tags
Apr
2
revised Experience with Detox Cloning
adding details
Apr
1
comment 205 nm UV-Vis readings
@MattDMo, I didn't want to lead the question but I was looking over this paper. There is a scientific basis for the quetion. onlinelibrary.wiley.com/doi/10.1002/pro.2253/abstract