4,167 reputation
1335
bio website
location
age
visits member for 2 years, 7 months
seen Aug 12 at 21:34

PhD student in chemical engineering


Mar
29
asked 205 nm UV-Vis readings
Mar
29
comment DNA gel extraction: chemical contaminants
Have you tried using alternative DNA measurement methods like a Qubit?
Mar
29
asked Experience with Detox Cloning
Mar
29
awarded  Nice Question
Mar
21
comment What is a simple protocol for staining cells in suspension?
Are we talking about mammalian cells or bacteria?
Mar
21
revised Improving Gel Extraction yields
added 87 characters in body
Mar
21
asked Improving Gel Extraction yields
Mar
20
asked How does LCR compare to Assembly PCR
Feb
15
awarded  Popular Question
Feb
14
comment Resuspending Cells from a filter plate
@Mattdmo, these are suspension cells so trypsinization will kill them. I'm using a vacuum manifold. I'm fearing the same thing which is why I'm doing this experiment to see if I can complete th mass balance
Feb
12
asked Resuspending Cells from a filter plate
Feb
12
comment Finding proteins in DNA sequence
Aren't the RBS and Shine Delgarno sequences a much better search term?
Feb
8
comment Does an allergic reaction kill body cells?
I would imagine that the complement system may trigger cell death.
Feb
5
revised What software is there available for Quantitative structure–activity relationship (QSAR) pharmacological studies?
adding tags
Jan
22
comment Is DNA green viewer carcinogenic?
How do you image the dye? SYBR and EtBr use difference wavelengths.
Jan
22
awarded  Yearling
Jan
16
awarded  Nice Question
Jan
16
accepted Low temperature PCR
Jan
16
awarded  Popular Question
Jan
12
comment What is the extent of the effect of Tris on E. coli?
Thanks! I had the same conclusions in regard to the dilution factor so I wasn't too worried. However, it's always better to have a good idea of the mechanism.