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seen Oct 13 at 21:13

PhD student in chemical engineering


Apr
9
comment What are the biological mechanisms behind the increase in cancer risk and alcohol consumption?
Asian Glow is linked with cancer. Actually tackling alchol related cancer is a fairly hot topic these days.
Apr
9
comment What are the biological mechanisms behind the increase in cancer risk and alcohol consumption?
I agree damaged is caused by acetaldehyde (also the cause of asian glow) but I believe that your selected enzyme is incorrect.
Apr
9
reviewed Approve suggested edit on How do the variable portions of antibody genes look in cells which don't produce antibodies?
Apr
9
reviewed Approve suggested edit on antibiotics tag wiki excerpt
Apr
9
comment How do you store membrane proteins?
@Suvidu, I would counter that if the purification protocol is a pain, it's a valuable investment to be able to scaleup effectively.
Apr
6
asked How do you store membrane proteins?
Apr
3
comment How prevalent is Taq polymerase in adding 3' A overhangs to the PCR product?
From what I understand, the A' overhang does eventually fall off. However, TA cloning is a much easier route if you have a TA vector.
Apr
2
revised How does Serotonergic (5-HT2A mediated) Psychedelia work?
edited tags
Apr
2
revised Experience with Detox Cloning
adding details
Apr
1
comment 205 nm UV-Vis readings
@MattDMo, I didn't want to lead the question but I was looking over this paper. There is a scientific basis for the quetion. onlinelibrary.wiley.com/doi/10.1002/pro.2253/abstract
Mar
29
asked 205 nm UV-Vis readings
Mar
29
comment DNA gel extraction: chemical contaminants
Have you tried using alternative DNA measurement methods like a Qubit?
Mar
29
asked Experience with Detox Cloning
Mar
29
awarded  Nice Question
Mar
21
comment What is a simple protocol for staining cells in suspension?
Are we talking about mammalian cells or bacteria?
Mar
21
revised Improving Gel Extraction yields
added 87 characters in body
Mar
21
asked Improving Gel Extraction yields
Mar
20
asked How does LCR compare to Assembly PCR
Feb
15
awarded  Popular Question
Feb
14
comment Resuspending Cells from a filter plate
@Mattdmo, these are suspension cells so trypsinization will kill them. I'm using a vacuum manifold. I'm fearing the same thing which is why I'm doing this experiment to see if I can complete th mass balance