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visits member for 2 years, 3 months
seen Apr 12 at 23:27

PhD student in chemical engineering


Feb
15
awarded  Popular Question
Feb
14
comment Resuspending Cells from a filter plate
@Mattdmo, these are suspension cells so trypsinization will kill them. I'm using a vacuum manifold. I'm fearing the same thing which is why I'm doing this experiment to see if I can complete th mass balance
Feb
12
asked Resuspending Cells from a filter plate
Feb
12
comment Finding proteins in DNA sequence
Aren't the RBS and Shine Delgarno sequences a much better search term?
Feb
8
comment Does an allergic reaction kill body cells?
I would imagine that the complement system may trigger cell death.
Feb
5
revised What software is there available for Quantitative structure–activity relationship (QSAR) pharmacological studies?
adding tags
Jan
22
comment Is DNA green viewer carcinogenic?
How do you image the dye? SYBR and EtBr use difference wavelengths.
Jan
22
awarded  Yearling
Jan
16
awarded  Nice Question
Jan
16
accepted Low temperature PCR
Jan
16
awarded  Popular Question
Jan
12
comment What is the extent of the effect of Tris on E. coli?
Thanks! I had the same conclusions in regard to the dilution factor so I wasn't too worried. However, it's always better to have a good idea of the mechanism.
Jan
10
asked What is the extent of the effect of Tris on E. coli?
Jan
8
asked What causes Acne?
Dec
16
comment Proteins that are strongly overproduced in E. coli and S. cerevisiae?
@Leonardo, CMV in ecoli probably would be a bad idea ;). I've heard that typically 1/6 of the cell weight would be protein so 20% doesn't sound too crazy.
Dec
13
revised What is the function of the RNA primer in DNA replication?
edited tags
Dec
10
comment Can compounds be predicted from Polyketide synthases gene clusters?
@Gergana Vandova, we were talking about whether or not Warp Drive Bio and Radiant have a realistic pipedream or not. I think that if they focus on PKS type Is, they might pull it off.
Dec
8
asked Can compounds be predicted from Polyketide synthases gene clusters?
Dec
5
comment When running a gel, what could cause a standard to run “faster” than usual?
Are you sure you have complete digest of the vector?
Dec
1
accepted What is a good list of unsolved protein structures?