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PhD student in chemical engineering


Jan
8
asked What causes Acne?
Dec
16
comment Proteins that are strongly overproduced in E. coli and S. cerevisiae?
@Leonardo, CMV in ecoli probably would be a bad idea ;). I've heard that typically 1/6 of the cell weight would be protein so 20% doesn't sound too crazy.
Dec
13
revised What is the function of the RNA primer in DNA replication?
edited tags
Dec
10
comment Can compounds be predicted from Polyketide synthases gene clusters?
@Gergana Vandova, we were talking about whether or not Warp Drive Bio and Radiant have a realistic pipedream or not. I think that if they focus on PKS type Is, they might pull it off.
Dec
8
asked Can compounds be predicted from Polyketide synthases gene clusters?
Dec
5
comment When running a gel, what could cause a standard to run “faster” than usual?
Are you sure you have complete digest of the vector?
Dec
1
accepted What is a good list of unsolved protein structures?
Nov
28
comment Low temperature PCR
If there was an easy solution, I wouldn't be asking it on SE.
Nov
28
comment What is a good list of unsolved protein structures?
@leonardo, well that seems to be a challenge.
Nov
28
comment Low temperature PCR
Well you can change the melting temperature using additives like DMSO and betaine. Unfortunately, the beads have to be coated and the polystyrene seems to be the best for the downstream steps so we would prefer to stick with polystyrene before re-optimizing everything else.
Nov
27
comment What is a good list of unsolved protein structures?
@terdon, that pretty much sounds like what I'm looking for. Unfortunately, there might be a significant number of proteins that have analogs in other species but essentially the same structure. Probably the safe test would be to look at the unsolved structures in ecoli.
Nov
27
asked Low temperature PCR
Nov
27
asked What is a good list of unsolved protein structures?
Nov
21
comment How can I compare rates of evolution for two sets of proteins?
I think you'll have to build the MSA regardless of the strategy that you use. You could try faster tree-based methods.
Nov
21
reviewed Approve suggested edit on Where does an organism store reserves of amino acids?
Nov
16
revised Isotype control antibodies in Flow Cytometry
Constant is the term that was looking for.
Nov
1
comment Why is it sometimes difficult to resuspend E. coli in P1?
since we're going into specifics with experimental protocols, I might as well get a precise answer. The Qiagen manual suggests 6,000xg for 15 minutes for Maxipreps and 6,800xg for 3 minutes for minipreps. Sounds like the geometry of rotors may make a small difference but that shouldn't affect the sedimentation velocity.
Oct
31
revised Why is it sometimes difficult to resuspend E. coli in P1?
edited tags
Oct
31
comment Why is it sometimes difficult to resuspend E. coli in P1?
An interesting addendum, I can pellet my cells for 3 minutes @ 1,000 g.
Oct
31
answered Can Bioluminescence drive photosynthesis?