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| visits | member for | 1 year, 4 months |
| seen | May 16 at 17:53 | |
| stats | profile views | 56 |
PhD student in chemical engineering
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Apr 17 |
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Are there any examples of sudden leaps in evolution? I believe that the technical term is a "Hard Sweep". |
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Apr 10 |
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What is the translation termination efficiency in mammalian cells? From what I understand the eRFs are not perfect but they still work better than RF1 and RF2 which allow for a non trivial amount of readthrough. |
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Apr 10 |
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What biochemical molecule viewer allows for changes in amino acids and resulting tertiary structure? Pymol should have that capability... try Gromacs |
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Apr 9 |
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What are the biological mechanisms behind the increase in cancer risk and alcohol consumption? Asian Glow is linked with cancer. Actually tackling alchol related cancer is a fairly hot topic these days. |
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Apr 9 |
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What are the biological mechanisms behind the increase in cancer risk and alcohol consumption? I agree damaged is caused by acetaldehyde (also the cause of asian glow) but I believe that your selected enzyme is incorrect. |
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Apr 9 |
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How do you store membrane proteins? @Suvidu, I would counter that if the purification protocol is a pain, it's a valuable investment to be able to scaleup effectively. |
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Apr 3 |
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How prevalent is Taq polymerase in adding 3' A overhangs to the PCR product? From what I understand, the A' overhang does eventually fall off. However, TA cloning is a much easier route if you have a TA vector. |
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Apr 1 |
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205 nm UV-Vis readings @MattDMo, I didn't want to lead the question but I was looking over this paper. There is a scientific basis for the quetion. onlinelibrary.wiley.com/doi/10.1002/pro.2253/abstract |
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Mar 29 |
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DNA gel extraction: chemical contaminants Have you tried using alternative DNA measurement methods like a Qubit? |
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Mar 21 |
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What is a simple protocol for staining cells in suspension? Are we talking about mammalian cells or bacteria? |
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Feb 14 |
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Resuspending Cells from a filter plate @Mattdmo, these are suspension cells so trypsinization will kill them. I'm using a vacuum manifold. I'm fearing the same thing which is why I'm doing this experiment to see if I can complete th mass balance |
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Feb 12 |
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Finding proteins in DNA sequence Aren't the RBS and Shine Delgarno sequences a much better search term? |
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Feb 8 |
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Does an allergic reaction kill body cells? I would imagine that the complement system may trigger cell death. |
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Jan 22 |
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Is DNA green viewer carcinogenic? How do you image the dye? SYBR and EtBr use difference wavelengths. |
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Jan 12 |
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What is the extent of the effect of Tris on E. coli? Thanks! I had the same conclusions in regard to the dilution factor so I wasn't too worried. However, it's always better to have a good idea of the mechanism. |
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Dec 16 |
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Proteins that are strongly overproduced in E. coli and S. cerevisiae? @Leonardo, CMV in ecoli probably would be a bad idea ;). I've heard that typically 1/6 of the cell weight would be protein so 20% doesn't sound too crazy. |
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Dec 10 |
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Can compounds be predicted from Polyketide synthases gene clusters? @Gergana Vandova, we were talking about whether or not Warp Drive Bio and Radiant have a realistic pipedream or not. I think that if they focus on PKS type Is, they might pull it off. |
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Dec 5 |
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When running a gel, what could cause a standard to run “faster” than usual? Are you sure you have complete digest of the vector? |
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Nov 28 |
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Low temperature PCR If there was an easy solution, I wouldn't be asking it on SE. |
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Nov 28 |
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What is a good list of unsolved protein structures? @leonardo, well that seems to be a challenge. |
