Reputation
4,711
Top tag
Next privilege 5,000 Rep.
Approve tag wiki edits
Badges
18 51
Newest
 Nice Answer
Impact
~140k people reached

Aug
7
comment Dimensionless number for blood volume
@VanceAlbaugh, Tau, Damkholer, f-number, Knudsen number, Radians, etc. Nondimensionalization is pretty standard in a lot of engineering fields. I would guess that BV is a good factor to normalize against. That way you can say that a "heart contains 0.05 BV" or "there is a flux of 0.01 BV/minute through this artery".
Jul
27
comment Dimensionless number for blood volume
@vancealbaugh, neither is time and length yet we frequently find ways to nondimensionlize those properties.
Jul
21
comment Dimensionless number for blood volume
Thanks for answering but at @WYSIWYG mentioned, it doesn't really answer.
Jul
21
comment Neutralizing TCA washes
How can I migrate this question?
Jun
3
comment Why do cows (and other hooved animals) need a hierarchy?
@fileunderwater, I frankly disagree. Meaning that a question covers a large amount of space should not rule out its answer-ability. If the question contained multiple questions, then I would agree. However, this is directly asking for the social hierarchies of herd animals which I think is a well bound question.
May
18
comment Why do cows (and other hooved animals) need a hierarchy?
Why is this closed? The question is super clear and well bound.
Feb
6
comment What temperature should mammalian B-Cells be stored at outside of the incubator?
Also, why are people collapsing questions from 2 years ago?
Feb
6
comment What temperature should mammalian B-Cells be stored at outside of the incubator?
@Rhill45, really? would say that part of the answer does involve "how long can you keep cell on ice". For instance, if you're going to FACS, what processing steps can you do before running them through the machine, what is the rate of aggregration, etc
Jan
28
comment Is bootstrapping an acceptable way to determine standard error for binding constants?
I approve this move. @fileunderwater Please let me know how to make that transition. I was under the impression that this was a fairly regular question that was relevant to the biochemistry community?
Oct
13
comment Neutralizing TCA washes
I accepted it since I was the one who asked the question and wrote the answer. It was downvoted since someone decided that it was "unsafe" despite the fact that this is actually the EH&S determined protocol. There needs to be better comment history on SE.
Jul
28
comment Phagemid display
Wow! Thanks for answering such an old question. I'm not entirely sure if this solves the avidity issues though.
Apr
3
comment E coli cotransformation efficiency
I've always understood for this process to be incredibly low. When you consider how much DNA is added and how many competent cells there are, if you have even 1% transformation efficiency, you'll always get lawns. Since we don't, that gives a rough estimate of the process.
Mar
4
comment Does the MS2 RNA binding protein have any translational repression effects?
Probably because I asked this question back in 2012 and was more of a curiosity question rather than an actual experimental question. More specifically, I'm concerned more about generating a tuneable analog type of repression system where you can generate a range of repress rather than perform a complete knockdown using CRISPR.
Mar
3
comment What is the fastest way to build an alanine scanning library?
@GerganaVandova, not really but it might be of interest to the broader community.
Mar
3
comment Does the MS2 RNA binding protein have any translational repression effects?
The idea would actually be to repress translation via the mecahnism that you suggest. Hence, the rationale of putting the MS2 hairpin upstream of the ORF.
Mar
3
comment What implications has the missing 2'-OH on the capability of DNA to form 3D structures?
This answer is incorrect for a variety of reasons. For one, it makes the assessment that RNA is more flexible. It isn't; DNA is. The role described in the Aleadam paper thus is minimal. The comments about degradation has little to do with the ability to adopt 3D structures. It is more of a reflection of the presence of specific defensive mechanisms via DNases and RNases.
Jan
21
comment How straightforward is in vitro compartmentalization?
Thanks! Since it has been awhile since I've asked this question, I now get the impression that certain reactions like transcription and smPCR work well. When concentrations and size become more important or translation is important things go south.
Dec
13
comment Is there a PSI-BLAST for nucleotide sequences?
why not blastn?
Dec
11
comment Finding RNA Loops
As an improvement to what @WYSIWYG mentioned, PARS is fairly low resolution since it is still enzyme based. The newer in vivo SHAPE or DMS-seq methods are much better and more informative.
Sep
6
comment Does RNA polymerase move around DNA or does DNA rotate benath the polymerase?
I think he is asking a different question. At some point the RNA should be wound around the DNA if the RNAP is spinning around the DNA.