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Mar
26
comment RNA migrating slower than DNA on Formaldehyde Gel?
While I agree with everything you mentioned, it doesn't really address the issue. I've been working on a standard curve. It is odd that the DNA is migrating much quicker than expected, as you point out it should be slower for the same length, doubly so for twice the length. And yet under multiple conditions I am seeing the same thing. Might be the long poly-A tracks in the DNA, I don't know. At this point it's not as import for the project as it is a curiosity.
Mar
26
accepted RNA migrating slower than DNA on Formaldehyde Gel?
Mar
26
comment How long can Cholera bacteria survive in a dead host?
None of what you mentioned is likely to have a infectious dose of v. cholerae. It's more a problem of directly using the water in your day to day life.
Mar
18
comment Congenital blindness due to retinitis pigmentosa - does it exist?
Thank you for linking the article. I think I now understand where this question is coming from. Was this prompted by Table 1? Because even if RP is not showing symptoms at an early age, you are still "born with it." As a genetic disorder, you are almost always going to see onset written as birth.
Mar
18
revised Congenital blindness due to retinitis pigmentosa - does it exist?
Added DOI
Mar
17
reviewed Reviewed Is the amount of blood that flows through every vein and artery per minute really a constant?
Mar
17
comment Congenital blindness due to retinitis pigmentosa - does it exist?
Just to be sure I understand the question, you're looking for case studies on a neonate that has already had a near total loss (or total loss) of vision from RP? I'm not sure it would be diagnosed as RP at that point.
Mar
16
comment RNA migrating slower than DNA on Formaldehyde Gel?
@Kendall That's a good idea. We're giving it another go. Oddly enough the RNA seems to be running the right size, it's the DNA that's migrating to far.
Mar
13
comment RNA migrating slower than DNA on Formaldehyde Gel?
They are not of equal size, that's the question. The RNA is 1/2 the length of the DNA.
Mar
13
comment “Enhancers” of enhancers?
Do you care if it's from a viral genome?
Mar
13
reviewed Close What are genome wide functional linkages?
Mar
13
reviewed Close Why doesn't HDL cause diseases like LDL?
Mar
13
reviewed Close How long the homologous arms of the “repair” sequence must be to do homologous recombination?
Mar
13
comment RNA migrating slower than DNA on Formaldehyde Gel?
@Pao 15.3% v/v formaldehyde, 41.3% v/v di formamide; updated question. Think that should be denaturing, and does SDS actually help denature RNA? That's probably off topic though.
Mar
13
comment RNA migrating slower than DNA on Formaldehyde Gel?
@Kendall Gel was 1M formaldehyde, 1% agarose. Run at 100V for 1.5h; updated question
Mar
13
revised RNA migrating slower than DNA on Formaldehyde Gel?
added detail
Mar
13
awarded  Convention
Mar
12
reviewed Close Is Sinus node conduction necessary for heart beating?
Mar
12
comment Is Sinus node conduction necessary for heart beating?
Do you have any evidence that it can "beat" properly on just the AVN? Loss of the SA node almost always leads to irregular rhythms very quickly (and with relatively bad outlook). Can you look up some complete heart block examples and see if that covers your question?
Mar
12
revised Balanced vs Maximized Codon Optimization
Fixed Links