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visits member for 1 year, 5 months
seen 7 hours ago

I'm currently collecting more letters after my name, because I am told these help you exchange your time for things you want.

Edit in response to changes: received more letters after my name, has not yet translated in to better exchange of my time for things I want. With an n of 2, I am probably going to have try for a third set of appending letters.

I have had no formal education in programming. All the programming I do is tangential to what I am trying to do, but I'll be damned if I let segfault stop me.

Thanks to all coders who help me. Come by Bio SE to see if I can help you.

When I say I have had no formal education in programming, I mean I've had absolutely no training in any computer science, and no one I work with has any training. I've used Debian, Biopup, SPSS, and R for years for what ever that's worth (again totally self taught).

Be warned that following any answers I give may lead to infected lemurs coming out of your computer and attacking you at night.

Happy coding.


22h
comment Skin porosity in glans penis when erect
@x457812 If you're interested in more of the bio-physics, I recommend reading up on the elastic fibers of the dermis. Incidentally they are quite abundant in the glans penis.
1d
reviewed Reviewed Can anyone identify this chrysalis? (Identified)
1d
reviewed Close TAA- Tumor associated antigen
1d
revised Skin porosity in glans penis when erect
added 44 characters in body
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revised Skin porosity in glans penis when erect
added 3 characters in body
1d
revised Skin porosity in glans penis when erect
added 191 characters in body
1d
comment Skin porosity in glans penis when erect
Diabetes is actually a good way to get an infection w/o risky sex, just as an aside.
1d
answered Skin porosity in glans penis when erect
Dec
18
comment Balanced vs Maximized Codon Optimization
Thanks for the answer. Small editing note, you included the "]" in several of the links (I could change it for you, but as new user you might want the practice). Looking at references, none seem to be advocating matching the levels found in the host (even if it's E. Coli). As far as the sequences, we're making about 14 or so different strains of a poly-protein, so that's probably not practical.
Dec
16
comment Balanced vs Maximized Codon Optimization
@WYSIWYG I remain unconvinced that the rate limiting factor in many cases is the protein folding, but rather tRNA availability plays a much bigger role in production. Particularly considering time scales involved in active infection (let's say less than 48h as a high limit).
Dec
16
comment Balanced vs Maximized Codon Optimization
I suppose I mention Menzella solely as an example of what makes me weary to try something different. If I can reasonable suspect that it will end in failure, I'd just as soon not waste time or funds on it.
Dec
16
revised Balanced vs Maximized Codon Optimization
added 83 characters in body
Dec
16
comment Balanced vs Maximized Codon Optimization
@WYSIWYG I want to maximize protein expression in a human cell line. While I appreciate David's work, that's not what really is at play here. The empirical observation from the theories leaves more complex modeling to be done. I am mostly interested in the bold question, perhaps I included too much background.
Dec
16
asked Balanced vs Maximized Codon Optimization
Dec
2
awarded  Enlightened
Dec
2
awarded  Nice Answer
Nov
21
reviewed Approve If human eyes watch an area, what's the shape of its capturing shape? Rectangular? Half spherical?
Nov
21
reviewed Close What is possibly wrong in my gel electrophoresis when I didn't see bands of DNA ladder on gel?
Nov
21
comment What is possibly wrong in my gel electrophoresis when I didn't see bands of DNA ladder on gel?
I'm not talking about the ruler. I'm talking about what's fluorescing pink down on lane 1, starting from the left. That looks like poorly resolved and partially degraded ladder to me. What was your gel composition?
Nov
21
reviewed Close What is the current scientific position on the issue of human race?