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I'm currently collecting more letters after my name, because I am told these help you exchange your time for things you want.

Edit in response to changes: received more letters after my name, has not yet translated in to better exchange of my time for things I want. With an n of 2, I am probably going to have try for a third set of appending letters.

I have had no formal education in programming. All the programming I do is tangential to what I am trying to do, but I'll be damned if I let segfault stop me.

Thanks to all coders who help me. Come by Bio SE to see if I can help you.

When I say I have had no formal education in programming, I mean I've had absolutely no training in any computer science, and no one I work with has any training. I've used Debian, Biopup, SPSS, and R for years for what ever that's worth (again totally self taught).

Be warned that following any answers I give may lead to infected lemurs coming out of your computer and attacking you at night.

Happy coding.


3h
reviewed Close Is there a gene that starts meiosis 2?
3h
comment Why doesn't the human skin grow back exactly how it was before being damaged?
@Leck there are different kinds of scars (scaring) caused by different kinds of insults and treatments. In a healthy individual, your skin is constantly growing and replacing itself. It dies and sloughs off as new skin is formed under it. I'm still not sure what your question is asking for beyond the wiki link. After you've read some more (or perhaps now) please take another stab at wording your question, and we'll see if we can't get on the same page.
9h
answered Theoretically, what technique would one use to modify a virus so that it only affected a subset of the population?
10h
revised Mammal body density
Changed based on conflicting statements
10h
comment Mammal body density
@kmm that has been my experience as well (no air) when harvesting bone marrow in mice. I'm going to remove that because I'm now looking at what I think is conflicting statements.
1d
answered Mammal body density
1d
comment Mammal body density
Why do throat sacs not count? I'm actually thinking of aquatic mammals that can purposefully adjust buoyancy with gas exchange/ retia mirabilia / etc.
1d
comment No digested product bands, but markers are visible. What could be the reasons?
Agreed, seems like overall signal is week, so a stain/destain seems in order
1d
comment What equipment would one use to modify a virus?
I actually am an expert at this, and I have always wanted someone to use forward genetics in a well researched piece of fiction for what it's worth. Feel free to try and ask more targeted questions, and I'd be happy to answer. I would also like to point out that considerable amounts of time and failure are to be expected. Even on a proven recombinant system I would put 1 month minimum to recover, purify, titer, and sequence a new virus.
1d
revised Is it possible to combine live attenuated and non-live vaccines?
edited body
1d
comment Is it possible to combine live attenuated and non-live vaccines?
@Chris thanks for that. Sorry I went AWOL. Turns out I had to have surgery on myself for once. Truncated the answer I was going to write to what's below.
1d
answered Is it possible to combine live attenuated and non-live vaccines?
Jul
8
awarded  Yearling
Jul
2
awarded  Curious
Jun
5
comment Transportation of bacteria
@okarin names of the bacteria, are they GMO (transformed with a plasmid counts unfortunately), the transport media, and transport temp. Ie. NEB 10b's transformed with HIV-env in a glycerol stock transported on dry-ice.
Jun
4
comment Is it possible to combine live attenuated and non-live vaccines?
+1 It's not actually difficult, it's more a question of practicality (when the vaccinations are given), and which vaccines actually work (live attenuated almost always elicits a stronger immune response, but can have more complications). Working on a detailed answer now.
Jun
4
comment Transportation of bacteria
@TheLastWord That's a good link to what needs to be covered, but your later comment is not entirely correct, and might cause confusion as BSL is not directly correlated with Risk Group Classification
Jun
4
comment Transportation of bacteria
@okarin That's actually bad advise. I would actually recommend that you ship them ahead of yourself. Not al microbes are Biosafety level 1, and their BSL level is not actually what is considered (risk level by CDC in the US, IATA has set standards as well). You need to determine if your bacteria are UN2814, UN2900, or UN3373. IF they are not pathogenic (not just to humans, but animals) but are GMO then they will be UN3245. Remember dry ice by itself is considered a hazardous material. Post specifically what you're moving and I'll give you an answer.
Jun
4
comment Generic Abbreviation for Immunopanning?
@TheLastWord Thanks for looking. Sadly I already got the publication out where we didn't abbreviate despite my desperate desire to do so. And I was highly highly tempted to use iPan. Maybe after I have tenure.
May
29
revised Meta-Data Retrieval from Bio-Rad Images
edited tags