2,950 reputation
629
bio website flavors.me/randogp
location Geneva, Switzerland
age
visits member for 2 years, 9 months
seen Apr 16 at 7:37

PhD in Pharmacology. Nuclear Receptors are my pet molecules and I look at them studying the relationships between nutritional messengers and gene expression. Twitter: @randogp


Jul
10
reviewed Approve suggested edit on Is Hydrogen gas present in biogas, and does it spontaneously ignite?
Jul
5
awarded  Custodian
Jul
5
reviewed No Action Needed Can ant colonies have two “branches”?
Jul
5
reviewed Approve suggested edit on Can ant colonies have two “branches”?
Jul
5
reviewed Reviewed What causes autism?
Jul
5
reviewed Leave Closed Suitable introductory book on Bioinformatics for a computer scientist?
Jul
3
reviewed Approve suggested edit on How to bind antibodies to study their properties?
Jul
3
reviewed Approve suggested edit on How does ubiquitin recognize misfolded proteins?
Jul
2
revised Selective Androgen Receptor Agonist
tagged
Jul
2
reviewed Approve suggested edit on Can PCR tubes be made of different materials than plastic?
Jul
2
reviewed No Action Needed Is there any documented evidence for suicide among vertebrates other than humans?
Jul
2
reviewed Approve suggested edit on Method to recognize the age of a firebug from the pattern on its back
Jul
1
reviewed No Action Needed What insect is this? (Black body two orange lines in its back and six legs)
Jul
1
comment Finding a template/oligo combination for my first PCR experiment
Using only two self-annealing oligos as both primers and template, you will just fill the in withouth amplification. The reaction will occurr even withouth thermal cycling, which renders the presence of thermocycler unuseful. Perhaps the worst experiment ever to do to test a thermocycler :-) Thanks @Alan Boyd for pointing it out. Btw, oligos are normally shipped lyophilized at room temperature in small tubes inside normal envelopes, ordering 2 or 4 will not affect shipping costs.
Jun
30
comment Finding a template/oligo combination for my first PCR experiment
@Alan, thanks I removed from the answer. Fill in, will not be useful to test the thermocycler.
Jun
30
revised Finding a template/oligo combination for my first PCR experiment
fixed conceptual mistake
Jun
30
comment Finding a template/oligo combination for my first PCR experiment
I edited my answer: you can do PCR with two oligo without any other DNA template. This is the simplest way I think.
Jun
30
revised Finding a template/oligo combination for my first PCR experiment
improved the answer with more details
Jun
30
answered Finding a template/oligo combination for my first PCR experiment
Jun
29
reviewed Reviewed Coiling of chromatids during cell division