2,970 reputation
629
bio website flavors.me/randogp
location Geneva, Switzerland
age
visits member for 2 years, 10 months
seen Apr 16 at 7:37

PhD in Pharmacology. Nuclear Receptors are my pet molecules and I look at them studying the relationships between nutritional messengers and gene expression. Twitter: @randogp


Aug
21
comment Does pain scale with mammal mass?
@skymninge there are at least 40 [animal models of neuropathic] (ncbi.nlm.nih.gov/pubmed/20030738), but I'm not inside the field and cannot judge if some of them can be used for allometric studies.
Aug
13
comment Are SLC52A2 and GPR172A really the same?
Very nice answer, thanks.
Jul
16
comment High Current (Speed) Transfer Buffer Recipe
I would look both into the MSDS sheet and, if any, the patent. They should help you to identify the components but not the recipe.
Jul
12
comment Preparation of normal DNA polymerase
I guess this is still related to your DIY project, you should specify it, otherwise people will assume you are in a standard lab with full access to state of the art equipment. 'Normal' DNA polymerase is not thermostable. It will be inactivated at the first cycle of PCR, that's why most polymerase we currently use comes from organisms that live in very hot environments.
Jul
1
comment Finding a template/oligo combination for my first PCR experiment
Using only two self-annealing oligos as both primers and template, you will just fill the in withouth amplification. The reaction will occurr even withouth thermal cycling, which renders the presence of thermocycler unuseful. Perhaps the worst experiment ever to do to test a thermocycler :-) Thanks @Alan Boyd for pointing it out. Btw, oligos are normally shipped lyophilized at room temperature in small tubes inside normal envelopes, ordering 2 or 4 will not affect shipping costs.
Jun
30
comment Finding a template/oligo combination for my first PCR experiment
@Alan, thanks I removed from the answer. Fill in, will not be useful to test the thermocycler.
Jun
30
comment Finding a template/oligo combination for my first PCR experiment
I edited my answer: you can do PCR with two oligo without any other DNA template. This is the simplest way I think.
Jun
27
comment Can life subsist on anything else but water?
Some scientists are indeed working toward inorganic life see here, bottom part‌​. Not that they succeeded yet, but it's an active research field.
Jun
26
comment What are the biological and nutritional benefits of having numerous smaller meals a day?
thank you NeoDevlin, nice addition.
May
30
comment In vitro enzyme production
yes, rabbit reticulocyte, wheat germs or Hela extracts. There are detailed steps on Current Protocols.
May
26
comment In vitro enzyme production
Don't take me wrong, but if you don't know where to start, kits are probably the worst starting point. It is not by mixing unknown solution A with proprietary buffer B that you will understand in vitro enzyme production. Once you master and troubleshoot the technique, using kits as pipetting shortcuts is fine.
May
16
comment Is wiping with RNAse Zap enough to destroy RNAse activity?
The MSDS reports to contain only a 'trade secret' surfactant in the range 1-20%. Why you say it is not a detergent?
Apr
23
comment Why is yawning contagious?
Are you looking for a causative factor (i.e, a neural circuit connects external yawing cues to the internal yawning effector center) or are you interested in the physiological/evolutionary reason (i.e., yawning communicates hypoxic environment to other individuals in the nearby).
Apr
19
comment PEG-silane treatment: why incubate for 18 hours at 60 degrees Celsius?
Google Images: glass chemical structure
Dec
5
comment What are the advantages and disadvantages of using beta-galactosidase compared to luciferase as a reporter gene?
Here a list of other luciferase assay shortcomings.
Nov
3
comment Do omnivore mammals vary food preferences based on dietary needs?
not sure amino acids can be considered micronutrients like vitamins.
Nov
1
comment Is there any biological reason that professional swimming is dominated by white people?
+1 the question is not racist, but I suggest to edit the question and replace 'race' with 'phenotype'. I'm sure there is a good cultural explanation, but this does not rule out genetic basis: Asian people get drunk easily because of the DNA. Looking forward for a GWAS study of olympic players: it will be very interesting!
Sep
24
comment Is there sufficient evidence that human cells are not intelligent?
something you would need to clarify is your definition of intelligence
Sep
21
comment Is there any documented evidence for suicide among vertebrates other than humans?
I would be curious to check if unicellular organisms commit apoptosis.
Sep
8
comment Does red light preserve your night vision?
For the negative votes: thanks. I edited the answer to be more on target.