| bio | website | linkedin.com/pub/… |
|---|---|---|
| location | Albany, NY | |
| age | 38 | |
| visits | member for | 1 year, 5 months |
| seen | Dec 14 '12 at 23:58 | |
| stats | profile views | 27 |
I have a background in cell biology research. My previous focus was in studying cancer progression and metastasis. I am now interested in mechanisms of inflammation, from a vascular physiology perspective.
In my free time (not much) I'm an amateur programmer (java, c#, javascript) and cell phone modder (ex winmo, now android).
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Jan 20 |
answered | How is eye color in humans inherited? |
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Jan 20 |
answered | Does current evidence support the use of resveratrol as an anti-ageing drug? |
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Jan 18 |
answered | How can I produce milligram quantities of an isotope-labeled DNA oligomer? |
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Jan 16 |
answered | Overlapping genetic information in eukaryotes |
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Jan 13 |
comment |
Reverse transcription PCR optimization I agree with Gergana, this is a PCR question. I also believe that a PCR tag should be enough for all its variations (RT-, Q-, nested, etc) |
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Jan 12 |
answered | The effect of the start codon GTG on translation in E. coli |
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Jan 12 |
answered | Reverse transcription PCR optimization |
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Jan 11 |
comment |
Is the squid giant axon the fastest conducting unmyelinated axon known? No, that's just one factor, but thought to be more variable than others, such as membrane capacitance or cytoplasm resistance, that are less variable among different cell types. |
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Jan 10 |
answered | Is the squid giant axon the fastest conducting unmyelinated axon known? |
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Dec 29 |
awarded | Critic |
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Dec 23 |
awarded | Editor |
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Dec 23 |
revised |
What implications has the missing 2'-OH on the capability of DNA to form 3D structures? added 70 characters in body |
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Dec 23 |
comment |
What is the Giemsa staining of chromosomes? @Masi in any other phase of the cell cycle, the DNA is much less condensed (called chromatin) and basically all DNA is intermingled, rendering a much more homogeneous staining. You still see regions of condensed chromatin, but it's impossible to identify individual chromosomes. You may do some (very limited) in situ hibridization, but again it's much more informative when cells are in metaphase. |
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Dec 22 |
answered | How can I measure bacterial alkaline phosphatase activity? |
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Dec 22 |
comment |
What do the variants on the PolyT sequence mean? A search in both PubMed and OMIM databases did not yield any result for the 6T polymorphism. It might be not yet described. If you are really interested in the molecular details, you'll have a compendium of mostly all what is known here: omim.org/entry/602421 |
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Dec 22 |
comment |
What is the Giemsa staining of chromosomes? Giemsa is too specific for its use as a tag. Staining and Histology are enough. Maybe Karyotype? |
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Dec 22 |
answered | What is the Giemsa staining of chromosomes? |
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Dec 22 |
answered | What implications has the missing 2'-OH on the capability of DNA to form 3D structures? |
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Dec 21 |
awarded | Beta |
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Dec 20 |
comment |
How can I keep HEK cells alive while expressing NMDA receptors? Sure, you can give that a try. I would expect that to lower the transfection efficiency rather than the level of expression per cell, but it's easy enough to try it right away. Good luck! BTW: I had to google "Heath Robinson": nice one! |