1,628 reputation
511
bio website linkedin.com/pub/…
location Albany, NY
age 40
visits member for 2 years, 10 months
seen Jan 1 at 5:39

I have a background in cell biology research. My previous focus was in studying cancer progression and metastasis. I am now interested in mechanisms of inflammation, from a vascular physiology perspective.

In my free time (not much) I'm an amateur programmer (java, c#, javascript) and cell phone modder (ex winmo, now android).


Mar
28
comment Why does the T7 RNA Polymerase require a reducing environment ie. DTT
@jp89 it is slowly improving, with the PLoS effort, and NIH manuscript requirements, but there is still a long way to go
Mar
28
comment Can methylation from DNA get copied to RNA during transcription?
That evolutionary conservation seems very interesting, but the question is whether it is maintained somehow into the transcribed RNA
Mar
28
comment Why does the T7 RNA Polymerase require a reducing environment ie. DTT
@jp89 Thanks for the copy of the paper. I'm not sure though if this is allowed here, since it is breaking copyrights. Anyhow, for now I believe it is still fair use. Regarding to the question, I'd follow citations 59-62 from there (last paragraph on page 2/first on page 3). Cheers.
Jan
20
comment Does current evidence support the use of resveratrol as an anti-ageing drug?
Yes, that was huge. Nevertheless, I know very honest people (in other institutions) that have found interesting results using resveratrol both in vitro and in animal models. Although a revisit of the literature will be needed, the field should not be ignored by the acts of one rotten lab.
Jan
13
comment Reverse transcription PCR optimization
I agree with Gergana, this is a PCR question. I also believe that a PCR tag should be enough for all its variations (RT-, Q-, nested, etc)
Jan
11
comment Is the squid giant axon the fastest conducting unmyelinated axon known?
No, that's just one factor, but thought to be more variable than others, such as membrane capacitance or cytoplasm resistance, that are less variable among different cell types.
Dec
23
comment What is the Giemsa staining of chromosomes?
@Masi in any other phase of the cell cycle, the DNA is much less condensed (called chromatin) and basically all DNA is intermingled, rendering a much more homogeneous staining. You still see regions of condensed chromatin, but it's impossible to identify individual chromosomes. You may do some (very limited) in situ hibridization, but again it's much more informative when cells are in metaphase.
Dec
22
comment What do the variants on the PolyT sequence mean?
A search in both PubMed and OMIM databases did not yield any result for the 6T polymorphism. It might be not yet described. If you are really interested in the molecular details, you'll have a compendium of mostly all what is known here: omim.org/entry/602421
Dec
22
comment What is the Giemsa staining of chromosomes?
Giemsa is too specific for its use as a tag. Staining and Histology are enough. Maybe Karyotype?
Dec
20
comment How can I keep HEK cells alive while expressing NMDA receptors?
Sure, you can give that a try. I would expect that to lower the transfection efficiency rather than the level of expression per cell, but it's easy enough to try it right away. Good luck! BTW: I had to google "Heath Robinson": nice one!
Dec
19
comment How should I ship plasmids?
+1 Fast, easy and very stable even for overseas shipments.