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visits member for 9 months
seen Aug 2 at 15:33

Mar
8
comment X Linked Hardy Weinberg Equilibrium Problem
I get the same result. These type of questions can throw people off very easily, apparently including the people writing the answer keys!
Mar
7
awarded  Custodian
Mar
7
reviewed Edit suggested edit on Can humans eat grass?
Mar
7
revised Can humans eat grass?
fixed up spelling
Mar
4
comment Why will world population keep growing if all women have only 2.1 children
Sources for your fact about the 2.1 growth rate leading to 0 net population growth? What is the meaning of TFR?
Mar
3
comment What are the highest and lowest possible pH, paCO2, and HCO3 in the blood of living human?
Perhaps a more reasonable thing to find is the variance or standard deviation of the values of these quantities in normal patients.
Mar
3
comment What's the point of glycolysis in fermentation?
you said that glycolysis generates net 2 ATP. That means 2 ATP in excess of what you started with. Plus @Chinmay and Satwik's answers
Mar
3
comment What are the highest and lowest possible pH, paCO2, and HCO3 in the blood of living human?
How would we know the numbers for any possible living body? The best you could rely on would be individual data points from someone whose blood happened to be checked for those quantities (i.e. highest and lowest recorded concentrations). Not a controlled experiment. And no way to tell if that's the highest possible.
Mar
1
comment Capsule and Antiphagocytosis as a pathogenesis factor
You need to define your terms and notation. What are all of these terms (antiphagocytosis, AB, a/b, C')? Can you provide some context?
Feb
23
comment Microtiter Dish Biofilm Formation Assay- Pseudomonas and Crystal Violet
Could you provide more context? A citation to a paper that uses this assay?
Feb
22
comment Isopropanol precipitation of DNA - duration and magnitude of cold storage
I agree that for most applications, Fig. 2 shows that waiting a short time gives reasonable yields. But without seeing the data point for 15 hours, you're extrapolating, I can imagine plausible scenarios where that extrapolation could be false, and if you care about getting as much of your sample as possible, I would want to see evidence that waiting 15 hrs doesn't improve yields before I take that out of my protocol. Your experience with this seems more credible to me, although have you done the experiment where you compare yields from just mixing the sample and leaving the sample overnight?
Feb
22
comment Isopropanol precipitation of DNA - duration and magnitude of cold storage
@Chris I find your experience more credible than Fig. 2. What if the amount of precipitate over time looks like a Michaelis-Menten curve (dwb4.unl.edu/Chem/CHEM869K/CHEM869KLinks/www.curvefit.com/… change x-axis to time and y-axis to amount of precipitate)? Then a lot would rapidly precipitate out, but a significant fraction might take a long time to come out in a short time; you wouldn't necessarily see the difference between 5 min and 10 min, but you might notice a difference after 15 hr.
Feb
22
comment Isopropanol precipitation of DNA - duration and magnitude of cold storage
Why can you conclude that overnight incubations are superfluous when they only examined conditions up to 10 minutes? The 5-minute difference between incubating for 5 or 10 minutes seems pretty minor compared to the possibility of incubation for 15+ hours. In general, I'm not so sure I can rule out that temperature might also have a better effect if the samples were left for 15 hours as opposed to 10 minutes.
Feb
17
comment How to determine the actual flux in the human metabolic model of a cell line?
A metabolic flux is the rate at which a metabolite gets converted into another metabolite by an enzyme. Metabolites aren't coded for by genes, so gene expression data doesn't help determine metabolic flux, unless there is some feedback that will cause the concentrations of the various enzymes involved to change via gene expression. But that occurs on a timescale which is much slower than typical metabolic flux.
Feb
17
answered Does this equation have a typo? (Systems Biology, singular value decomposition)
Feb
16
comment Natural examples of XOR functions at the cellular level
What's interesting to me is that it's not hard to come up with XOR gate implementations that sound biologically plausible: gene X is activated by two separate activators A and B. When present at the same time A and B bind each other strongly in such a way that their DNA binding domains are occluded, decreasing the transcription of X. It would be a leaky XOR gate, but it seems pretty biologically plausible. But my cursory search didn't yield much on competing trans-activators that can heterodimerize...
Feb
16
awarded  Organizer
Feb
16
revised question about directional selection
Added the homework tag
Feb
16
revised What would happen if you combined the world's deadliest diseases & viruses?
Corrected spelling and fixed grammar
Feb
16
suggested suggested edit on What would happen if you combined the world's deadliest diseases & viruses?