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Oct
18
comment What is the allelic frequency at mutation-selection-drift equilibrium?
I think this paper might be relevant. From what I can see they derive an analytical approx for deleterious allele frequency in MSD equilibrium.ncbi.nlm.nih.gov/pmc/articles/PMC1894624
Oct
17
comment How do chromosomes connect?
There has been a lot of relatively recent work on 3D chromosome structure and physical interactions between regions of the chromosome. For an example, look up erez lieberman-Aiden et al's paper on Hi-C chromatin conformation capture.
Oct
12
answered Active & passive transport question
Sep
25
awarded  Enlightened
Sep
25
awarded  Nice Answer
Mar
8
comment X Linked Hardy Weinberg Equilibrium Problem
I get the same result. These type of questions can throw people off very easily, apparently including the people writing the answer keys!
Mar
7
awarded  Custodian
Mar
7
reviewed Edit suggested edit on Can humans eat grass?
Mar
7
revised Can humans eat grass?
fixed up spelling
Mar
4
comment Why will world population keep growing if all women have only 2.1 children
Sources for your fact about the 2.1 growth rate leading to 0 net population growth? What is the meaning of TFR?
Mar
3
comment What are the highest and lowest possible pH, paCO2 and HCO3 in the blood of living human?
Perhaps a more reasonable thing to find is the variance or standard deviation of the values of these quantities in normal patients.
Mar
3
comment What's the point of glycolysis in fermentation?
you said that glycolysis generates net 2 ATP. That means 2 ATP in excess of what you started with. Plus @Chinmay and Satwik's answers
Mar
3
comment What are the highest and lowest possible pH, paCO2 and HCO3 in the blood of living human?
How would we know the numbers for any possible living body? The best you could rely on would be individual data points from someone whose blood happened to be checked for those quantities (i.e. highest and lowest recorded concentrations). Not a controlled experiment. And no way to tell if that's the highest possible.
Mar
1
comment Capsule and Antiphagocytosis as a pathogenesis factor
You need to define your terms and notation. What are all of these terms (antiphagocytosis, AB, a/b, C')? Can you provide some context?
Feb
23
comment Microtiter Dish Biofilm Formation Assay- Pseudomonas and Crystal Violet
Could you provide more context? A citation to a paper that uses this assay?
Feb
22
comment Isopropanol precipitation of DNA - duration and magnitude of cold storage
I agree that for most applications, Fig. 2 shows that waiting a short time gives reasonable yields. But without seeing the data point for 15 hours, you're extrapolating, I can imagine plausible scenarios where that extrapolation could be false, and if you care about getting as much of your sample as possible, I would want to see evidence that waiting 15 hrs doesn't improve yields before I take that out of my protocol. Your experience with this seems more credible to me, although have you done the experiment where you compare yields from just mixing the sample and leaving the sample overnight?
Feb
22
comment Isopropanol precipitation of DNA - duration and magnitude of cold storage
@Chris I find your experience more credible than Fig. 2. What if the amount of precipitate over time looks like a Michaelis-Menten curve (dwb4.unl.edu/Chem/CHEM869K/CHEM869KLinks/www.curvefit.com/… change x-axis to time and y-axis to amount of precipitate)? Then a lot would rapidly precipitate out, but a significant fraction might take a long time to come out in a short time; you wouldn't necessarily see the difference between 5 min and 10 min, but you might notice a difference after 15 hr.
Feb
22
comment Isopropanol precipitation of DNA - duration and magnitude of cold storage
Why can you conclude that overnight incubations are superfluous when they only examined conditions up to 10 minutes? The 5-minute difference between incubating for 5 or 10 minutes seems pretty minor compared to the possibility of incubation for 15+ hours. In general, I'm not so sure I can rule out that temperature might also have a better effect if the samples were left for 15 hours as opposed to 10 minutes.
Feb
17
comment How to determine the actual flux in the human metabolic model of a cell line?
A metabolic flux is the rate at which a metabolite gets converted into another metabolite by an enzyme. Metabolites aren't coded for by genes, so gene expression data doesn't help determine metabolic flux, unless there is some feedback that will cause the concentrations of the various enzymes involved to change via gene expression. But that occurs on a timescale which is much slower than typical metabolic flux.
Feb
17
answered Does this equation have a typo? (Systems Biology, singular value decomposition)