637 reputation
412
bio website has100ideas.com
location San Francisco, CA
age 30
visits member for 2 years, 8 months
seen Jul 30 at 20:33

I am developing cheap, reliable, and simple molecular biology techniques and DIY-equipment for amateur biotechnologists.

I am one of the founders of a community of amateur biologists called diybio.org, for people who want to understand, and in some cases, re-engineer the living world.

My main objective is to help the amateur biotechnology movement develop in a safe and positive way. If you want to help, let me know by emailing contact@diybio.org


Dec
14
awarded  Yearling
Sep
18
awarded  Commentator
Sep
18
comment NADH vs. NADPH: Where is each one used and why that instead of the other?
Great use of the link to the chapter - I wish more authors did this.
Sep
5
answered How to secrete a recombinant protein from E. coli?
Aug
10
awarded  Popular Question
Dec
14
awarded  Yearling
Mar
19
awarded  Nice Question
Mar
17
awarded  Scholar
Mar
17
accepted What effect does vortexing have on a fluid sample that simple mechanical shaking does not?
Mar
16
comment What effect does vortexing have on a fluid sample that simple mechanical shaking does not?
Besides mixing fluids of different viscosities, are vortexers necessary for any other lab operations?
Mar
16
comment What effect does vortexing have on a fluid sample that simple mechanical shaking does not?
Vortexing is called for when one or more of the fluid samples is more viscous than water? For instance (as you suggest), 100% glycerol is 1000x more dynamically viscous than water, hence the need for a vortexer to mix water and glycerol well (wolframalpha.com/input/…).
Mar
16
awarded  Student
Mar
15
asked What effect does vortexing have on a fluid sample that simple mechanical shaking does not?
Jan
24
comment What is the criticality of the ribosome binding site relative to the start codon in prokaryotic translation?
It was an interesting question to investigate and I enjoyed learning about it. I hope more well-posed and provocative questions are asked here and that users react to them by actually going out and reading up on the primary literature. Looks like that happens occasionally. Cool.
Jan
10
awarded  Enlightened
Jan
10
awarded  Nice Answer
Dec
27
awarded  Critic
Dec
21
awarded  Beta
Dec
16
answered What conditions should I use for Gel Red staining?
Dec
16
comment What conditions should I use for Gel Red staining?
What wavelength and filter set are you using for visualizing your gels?