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Just another spaghetti chef.


1d
comment Long term storage of agarose-ethidium bromide gels that have already been cast
I can confirm storage of the bleach gel as well - not only that, but despite not taking care to keep the bleach gel away from RNAse contaminants (keeping in the same box as normal gels, dirty electrophoresis tank) my RNA ran and stained just fine.
1d
accepted Long term storage of agarose-ethidium bromide gels that have already been cast
1d
comment Long term storage of agarose-ethidium bromide gels that have already been cast
@MarchHo To be sure, just because they are identical doesn't mean there will be no net diffusion.
2d
answered Long term storage of agarose-ethidium bromide gels that have already been cast
2d
accepted Why don't fish groom each other?
2d
comment Evolution: One big population vs. many small populations
While you are correct and make valid points, in retrospect I realize that I failed to adequately specify that I am interested in the biology, not the methodology. As such I neglect contamination risk, and apply the same conditions to all cultures. (this is more realistic, if you think of natural populations)
2d
comment Evolution: One big population vs. many small populations
Very helpful! I especially appreciate the links to literature, thanks.
2d
revised Evolution: One big population vs. many small populations
added 1 character in body
Dec
19
comment Mass spectrometry versus western blotting for validation
My impression is that Western is the gold standard that MS is measured against, but not rightly so. As you have witnessed, it's perfectly possible for Western to fail because of eg. poor antibodies when MS will perform fine. It is not necessarily better than MS. Generally, if MS and Western disagree, I don't think you should really just trust one (whether you would get away with it is a different matter). Either one could be an artifacts. Without additional experiments, it's very hard to say.
Dec
18
asked Evolution: One big population vs. many small populations
Dec
11
revised DIY extraction and long term storage of human DNA sample?
added 46 characters in body
Dec
11
comment What's up with all the vague protocols?
@Karolas Incidentally, the "boring lab questions" have always been a bit of an unclear case as to whether they fit. I believe a Meta discussion concluded that it's best to include them here, as opposed to say Academia@SE or a separate SE site. That said, the hold appears to be not for topicality, but subjectivity (and to be honest I don't understand how it's opinion based, but that's another matter).
Dec
10
reviewed Close Does our brain really “flip” images perceived by our eyes?
Dec
10
reviewed Close Can an influenza virus carry other infectors with it?
Dec
10
comment DIY extraction and long term storage of human DNA sample?
@user137 Doubt it. Phenol is needed to denature the proteins.
Dec
10
revised DIY extraction and long term storage of human DNA sample?
added 258 characters in body
Dec
10
comment DIY extraction and long term storage of human DNA sample?
@Chris Always consult the MSDS of the substances you work with and take appropriate safety precautions!
Dec
10
reviewed Leave Open Are stable proteins tagged by ubiquitin?
Dec
10
comment Are stable proteins tagged by ubiquitin?
Why do you expect it to get tagged by ubiquitin? Also, what kind of ubiquitination?
Dec
10
reviewed Close Why is important to get saturated fatty acids from diet?