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visits member for 2 years, 4 months
seen Jul 2 at 22:33

Jul
2
accepted How does heat shock transformation work?
Jul
2
awarded  Curious
Jun
20
comment How is the transcription direction of RNA polymerase determined?
I don't think transcription always goes $5' \rightarrow 3'$ wrt to coding strand. See en.wikipedia.org/wiki/…
Jun
14
revised What are these strings used to describe animals?
Added relevant tags
Jun
14
suggested suggested edit on What are these strings used to describe animals?
Jun
13
comment How do penicillin resistant bacteria grow slower in the presence of penicillin?
@daniel Assuming not every cell in the population expresses the exact same amount of lactamase, it stands to reason that some of them make more of it than others. It is well known that expressing arbitrary proteins, or antibiotic resistance, by itself constitutes an energy burden. I cannot provide references unless OP clarifies the experiment.
Jun
13
answered How do penicillin resistant bacteria grow slower in the presence of penicillin?
Jun
13
revised Equation for accurate prediction of PCR yield
deleted 12 characters in body
Jun
13
revised Equation for accurate prediction of PCR yield
added 5 characters in body
Jun
13
asked Equation for accurate prediction of PCR yield
Jun
13
answered Can 'hardware' thermalization explain memory decay?
Jun
13
comment Natural examples of XOR functions at the cellular level
Many sex-specific genes exist in a pretty much XOR state. Same for cell fate specification. Delta-notch and neurogenin could be an example, though it's not cell autonomous.
Jun
11
revised How does heat shock transformation work?
added 1398 characters in body
Jun
11
comment How does heat shock transformation work?
@AlanBoyd I have heard many scientists also claim that nobody knows. I have always found it bizarre. How can such a simple, popular technique not be well understood? Are the experimental techniques to study it not available? Or does nobody bother because they think even if they figure it out, nobody will cite it or care?
Jun
11
accepted What is the best file format to store gel images?
Jun
11
asked How does heat shock transformation work?
Jun
11
comment Optimization of annealing temperature
Most PCR enzymes come with a manual (which can also be found online) that has a troubleshooting section, often dealing exactly with you problem. It may be more fruitful to first follow these steps, and include the outcome in your question.
Jun
11
comment What is the best file format to store gel images?
I asked this here because an answer would require understanding of what sorts of information is recorded when a gel photo is taken, and how valuable they are for research purposes. I'm open to the idea of migrating it elsewhere (for instance SuperUser) if that seems more appopriate.
Jun
11
asked What is the best file format to store gel images?
Jun
5
awarded  Nice Answer