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visits member for 2 years, 9 months
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Nov
18
comment Predictive Single Nucleotide Polymorphisms (SNPs) for Bipolar Disorder
Yes, that's what I mean.
Nov
17
asked Predictive Single Nucleotide Polymorphisms (SNPs) for Bipolar Disorder
Nov
13
comment What are the features on a microscope one needs in order to do lab work?
Keep in mind that you will need it to have a camera to store the images, at least in a clinical or research setting. That will add quite a bit more expense. If you get creative, you can now use smartphone cameras for much less.
Nov
13
answered What are the features on a microscope one needs in order to do lab work?
Nov
5
comment Food allergen-related skin reactions and intestinal inflammation?
I don't know for certain that is the causal direction, but that is the direction I was intending when considering that immediate contact with the food allergen initiates an allergic reaction, so it may stand to reason (I'm not an immunologist), that if the food is ingested, may then later cause gastrointestinal problems.
Nov
5
comment Food allergen-related skin reactions and intestinal inflammation?
Oh, I see what you did then. No harm done.
Nov
4
comment Food allergen-related skin reactions and intestinal inflammation?
I don't see how that question is related. It deals with extra-intestinal diseases, of which, dermatologic conditions may be a subsequent symptom. This question deals with the reverse direction of causation. Skin contact -> intestinal issues.
Nov
4
asked Food allergen-related skin reactions and intestinal inflammation?
Oct
28
awarded  Nice Question
Oct
28
comment Why is coffee a laxative?
It's not valid to compare anecdotal (or real, for that matter) effects of those drugs you mention to a specific effect of caffeine just because they are broadly classed as stimulant drugs. Those two drugs all have distinct mechanisms of action to how they work. However, if they increase gut motility (e.g., peristalsis) then it may coincidentally also increase defecation.
Sep
30
awarded  Explainer
Sep
26
comment Death because of distilled water consumption
Sorry, I took the question to mean drinking distilled water only, and ingesting nothing else. I entirely agree that my previous answer was misleading, and @recurivelyironic and TonyH give much better answers for acute effects or drinking distilled water while still eating normally.
Sep
26
revised Death because of distilled water consumption
i wasn't clear enough in my answer
Sep
23
comment Troubleshooting SDS-PAGE of trypsin-treated BSA
The stacking gel should be 4%, and the separating gel is usually no greater than 16% (and that's to separate really large proteins). What molecular range bands are you expecting to see, and why is the separating gel percentage so high?
Sep
21
comment How can I determine the purity of cells isolated from rat brains if I cannot use FACS, Immunohistochemistry or SEM anaysis?
@WYSIWYG - I agree, but that means the technique is not only qualitative. Automated imaging and segmentation is the modern successor to random fields + stereology.
Sep
20
comment Machine learning for light microscopy — problems to solve?
Blind deconvolution is already a Bayesian algorithm of sorts. Scaled-gradient-projection deconvolution is a faster computational approach leading to similar or better increase in resolution. It's currently applied to optical microscopy and could be extended to super-resolution microscopy.
Sep
20
comment Machine learning for light microscopy — problems to solve?
Look into WEKA segmentation algorithms implemented in FIJI/ImageJ.
Sep
20
comment How can I determine the purity of cells isolated from rat brains if I cannot use FACS, Immunohistochemistry or SEM anaysis?
q-PCR would be the most accurate PCR technique if you wanted a breakdown of the population by percentage. Simple optical techniques are quantitative if you abide by stereology techniques.
Sep
2
answered What causes skewed ladder bands on an agarose gel?
Aug
30
revised Why is it sometimes difficult to resuspend E. coli in P1?
typo