meta user
network profile
| bio | website | guizzetti.ca |
|---|---|---|
| location | London, Ontario | |
| age | 26 | |
| visits | member for | 1 year, 2 months |
| seen | May 19 at 3:03 | |
| stats | profile views | 27 |
I'm Leo and I'm passionately involved in biology and running, and interested in programming and cooking.
|
May 3 |
comment |
How is gene expression estimated? @SteveLianoglou: MS is not my expertise, but that is not my understanding (this may vary with the type of MS used, e.g., TOF-SIM, GC). Successful identification of proteins via MS is dependent on abundance but also peptide fragment length (read: experimental optimization). MS can theoretically get down to 1 fmol resolution. Check this out for more information: lab.rockefeller.edu/chait/pdf/08/08_eriksson_wiley.pdf |
|
May 1 |
comment |
How is gene expression estimated? @WYSIWYG: no, I guess not, but it is certainly quantitative and highly sensitive, so I'm (lazily) throwing it in with the more traditional methods. ;) |
|
Apr 30 |
comment |
How is gene expression estimated? It's my understanding that scientists often use these microarray experiments as high-throughput screening methods to identify either candidate genes or gene networks. If the data from one such experiment is truly to be trusted, it must always be followed up by traditional biochemical methods (mass spec, western blot, ELISA/RIA, microscopy). |
|
Apr 29 |
reviewed | Approve suggested edit on Why do sloths come down from the trees to defecate? |
|
Apr 20 |
reviewed | Approve suggested edit on drosophila tag wiki excerpt |
|
Apr 20 |
reviewed | Approve suggested edit on western-blot tag wiki excerpt |
|
Apr 20 |
reviewed | Approve suggested edit on reverse-transcription tag wiki excerpt |
|
Apr 18 |
reviewed | Approve suggested edit on animal-behaviour tag wiki excerpt |
|
Apr 17 |
reviewed | Close Were any brain fusion experiments ever conducted? |
|
Apr 8 |
accepted | How to Design an siRNA Experiment? |
|
Mar 30 |
reviewed | Close What's the Evolutionary Purpose of Hair in Humans (namely the hair that grows on their skin, NOT internal hairs)? |
|
Mar 30 |
revised |
205 nm UV-Vis readings Edited for clarity. |
|
Mar 30 |
comment |
205 nm UV-Vis readings You would appear to be correct. What is not shown is relative absorbance of protein and nucleic acid at 205 nm. NB: I have not followed up with the two references linked from that page I posted. |
|
Mar 30 |
answered | 205 nm UV-Vis readings |
|
Mar 23 |
accepted | Complexity in creating transgenic animals (e.g., mice) |
|
Mar 22 |
comment |
Complexity in creating transgenic animals (e.g., mice) Hmm...I forgot to mention the obvious problem with combinatorial effects that you brought up. I was assuming that the effects of single mutations were already known. These are interesting points you bring up. |
|
Mar 22 |
asked | Complexity in creating transgenic animals (e.g., mice) |
|
Mar 22 |
reviewed | Close What are the main functions of AMPK protein in the intestine? |
|
Mar 22 |
reviewed | Approve suggested edit on Do dolphins really sleep? |
|
Mar 21 |
answered | RACE pcr product |
