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visits member for 2 years, 7 months
seen Oct 8 at 1:05

Sep
30
awarded  Explainer
Sep
26
comment Death because of distilled water consumption
Sorry, I took the question to mean drinking distilled water only, and ingesting nothing else. I entirely agree that my previous answer was misleading, and @recurivelyironic and TonyH give much better answers for acute effects or drinking distilled water while still eating normally.
Sep
26
revised Death because of distilled water consumption
i wasn't clear enough in my answer
Sep
23
comment Troubleshooting SDS-PAGE of trypsin-treated BSA
The stacking gel should be 4%, and the separating gel is usually no greater than 16% (and that's to separate really large proteins). What molecular range bands are you expecting to see, and why is the separating gel percentage so high?
Sep
21
comment How can I determine the purity of cells isolated from rat brains if I cannot use FACS, Immunohistochemistry or SEM anaysis?
@WYSIWYG - I agree, but that means the technique is not only qualitative. Automated imaging and segmentation is the modern successor to random fields + stereology.
Sep
20
comment Machine learning for light microscopy — problems to solve?
Blind deconvolution is already a Bayesian algorithm of sorts. Scaled-gradient-projection deconvolution is a faster computational approach leading to similar or better increase in resolution. It's currently applied to optical microscopy and could be extended to super-resolution microscopy.
Sep
20
comment Machine learning for light microscopy — problems to solve?
Look into WEKA segmentation algorithms implemented in FIJI/ImageJ.
Sep
20
comment How can I determine the purity of cells isolated from rat brains if I cannot use FACS, Immunohistochemistry or SEM anaysis?
q-PCR would be the most accurate PCR technique if you wanted a breakdown of the population by percentage. Simple optical techniques are quantitative if you abide by stereology techniques.
Sep
2
answered What causes skewed ladder bands on an agarose gel?
Aug
30
revised Why is it sometimes difficult to resuspend E. coli in P1?
typo
Aug
30
comment How does extracellular potassium ion concentration and calcium ion concentration affect the excitability of a cell?
Rory M - that's not always true about decreasing excitability. Case and point: > 25 mM K+ is a standard depolarizing agent in cell culture that triggers action potential firing. Also, a high K+ gradient across the membrane still leaks some K+ into the cell. Strictly speaking, this depends on the complement of voltage-gated and voltage-insensitive ion channels expressed on the cell surface.
Aug
30
comment Does scratched Teflon coated frying pans contain carcinogens which can cause cancer?
Hi there, I've tried to re-word your question to be more objective about the concern that Teflon (or some part of the pan) may contain possible carcinogens. I hope you are happy with this edit.
Aug
30
reviewed Edit Does scratched Teflon coated frying pans contain carcinogens which can cause cancer?
Aug
30
revised Does scratched Teflon coated frying pans contain carcinogens which can cause cancer?
re-worded question
Aug
26
revised can a bacterial protease inhibitor cocktail be used for Western Blotting involving HUVEC cells and HT-29 adenocarcinoma cells?
deleted 13 characters in body
Aug
26
reviewed Close Is it possible to convert nerve signals or electrical pulses into a readable format?
Aug
26
reviewed Leave Closed What are the dimensions in angstrom of bacteria?
Aug
26
answered can a bacterial protease inhibitor cocktail be used for Western Blotting involving HUVEC cells and HT-29 adenocarcinoma cells?
Aug
14
reviewed Leave Open Glutamine analysis
Jul
31
comment Verifying Protein Folds
That is what happens. These distributed computing platforms send out duplicate jobs for computation and the results compared after all are returned. It is also possible to get a sense of confidence in the fold prediction by comparing sequence homology to what is already known for solved protein structures.