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| bio | website | guizzetti.ca |
|---|---|---|
| location | London, Ontario | |
| age | 26 | |
| visits | member for | 1 year, 2 months |
| seen | yesterday | |
| stats | profile views | 27 |
I'm Leo and I'm passionately involved in biology and running, and interested in programming and cooking.
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Mar 21 |
comment |
What are usable Sum formulas for Proteins? Hi mart, I would say the edit was better, but (to me) you still don't seem clear about what you need. Considering sulfur in proteins, only cysteine (Cys) and methionine (Met) use S, and a subset of proteins are sulfonated. The proportion of Cys/Met in proteins varies widely, so 11-19% seems reasonable, and maybe even on the high end. Also keep in mind proteins are not static entities in a cell, they are always in flux, being broken down and synthesized. I'm not certain you can reduce it to a static chemical formula. |
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Mar 18 |
reviewed | Close Is there a future for fisheries? |
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Mar 18 |
comment |
Sequencing rtPCR product Theoretically, you will still end up with a specific PCR amplicon, so you can sequence it with the same primers used for rt-PCR. By the way, do you mean real-time or reverse transcriptase PCR? |
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Mar 15 |
reviewed | Approve suggested edit on Might a previously administered MPSV4 vaccine react with a newly administered MCV-4 vaccine? |
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Mar 14 |
reviewed | Approve suggested edit on Might a previously administered MPSV4 vaccine react with a newly administered MCV-4 vaccine? |
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Mar 12 |
revised |
Chromosomes are of different size but why do all chromosomes have similar GC percentage? Removed extraneous word trailing at the end of the comment. |
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Mar 12 |
comment |
Are descriptive statistics sometimes more useful than tests of significance? I think this is a valid question to as Biology because the standards of publication are different between fields of study. I'm sure any statistician will have problems with biological literature. You may have found an important, and biological effect which you can describe qualitatively. When it comes time to publish, if you cannot show the different quantitatively (and likely with a stats test), you limit yourself to publishing in a lower-tier journal. |
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Mar 11 |
comment |
Alternatives to TBE buffer for denaturing polyacrylamide gel electrophoresis of DNA and RNA? A neighbouring lab uses the sodium borate buffer for genotyping in agarose gels. They can run the gels at 10% higher voltages than what I push my TAE buffer at (95V; 20 mA max). Is there a particular reason to use denaturing poly-acrylamide or to run them super fast? |
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Feb 28 |
awarded | Yearling |
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Feb 16 |
reviewed | Leave Closed Is it true that oxygen has a diffusion rate in air 10,000 times greater than in water? |
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Feb 16 |
reviewed | Approve suggested edit on Does ethanol destroy RNase? |
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Feb 16 |
comment |
Does ethanol destroy RNase? In short, RNA contamination and degradation are super easy in a lab setting. We have an RNA-only dedicated workbench in our lab which gets treated with a "broad spectrum" RNAse-degrading solution before any RNA work is conducted. |
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Feb 3 |
reviewed | Approve suggested edit on How common is bacterial mediated transformation? In plants? In animal cells? |
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Jan 30 |
awarded | Revival |
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Jan 29 |
reviewed | Close Why people like to see pornographic scenes, but do not feel the same about eating scenes? |
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Jan 29 |
reviewed | Leave Open Microscopy Book Suggestions |
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Jan 29 |
reviewed | Approve suggested edit on Why am I getting low transformation efficiency with DB3.1 E.coli cells? |
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Jan 28 |
reviewed | Approve suggested edit on Microscopy Book Suggestions |
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Jan 21 |
reviewed | Approve suggested edit on Why can silicone reduce keloid? |
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Jan 21 |
reviewed | Approve suggested edit on How does toluene inhalation damage the brain? |
