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visits member for 2 years, 6 months
seen 18 hours ago

Oct
16
answered Are there any websites offering graphs for the light absorption of different enzymes?
Oct
10
comment Multi-nucleated cells: advantages and examples?
That's an example I was not aware of, but very interesting.
Oct
8
comment Does an annealing temp higher than primer's Tm contribute to primer dimer?
You can always use a lower Tm, but then you sacrifice specificity. In situations like these, I like using a gradient thermocycler to test a range of annealing temperatures to find the optimum.
Oct
8
awarded  Custodian
Oct
8
reviewed Reviewed What gaseous substances do humans emit?
Oct
8
answered Multi-nucleated cells: advantages and examples?
Oct
7
answered Where are -HCl supplements absorbed in the human digestive system?
Oct
1
comment Do Yeast Insertion constructs revert?
Disclaimer: I only work with bacteria. This seems analogous to antibiotic selection in E. coli, and as such, selection pressure is needed to maintain the engineered yeast population to be assured that you aren't incubating environmental strains.
Sep
21
awarded  Custodian
Sep
20
awarded  Enlightened
Sep
20
awarded  Nice Answer
Sep
13
awarded  Nice Question
Sep
12
comment How are DNA polymerase error rates measured?
Thank you for the reference. This seems like the most pragmatic approach to this problem. Even though by design this method under-reports the error rate, it would be a consistent bias across polymerases.
Sep
12
accepted How are DNA polymerase error rates measured?
Sep
12
comment How are DNA polymerase error rates measured?
This is why I was sceptical about a sequencing-based approach. I suppose the method described by Alan Boyd below is a functional approach, but this still masks synonymous mutations and therefore under-represents the true error rate.
Sep
12
answered Which enzyme catalyzes transcription and which translation?
Sep
11
comment Why do most breast cancers occur in women?
I agree that estrogen plays a very important role (among other hormonal cues). Another significant explanation that follows from your first example is the sheer quantity of female breasts to male breasts. While only some men have fatty breast tissue, all women do, and so the diagnoses are largely skewed by the anatomy. Something else to keep in mind is that women have breasts from puberty through the rest of their lives, while many men only develop them later in life.
Sep
11
asked How are DNA polymerase error rates measured?
Jun
23
comment Do all plants photosynthesize with equal efficiency?
It should also be clarified how exactly you define efficiency. Photosynthesis on a molecular level is likely highly efficient because the pigments exploit a quantum mechanical property called quantum coherence. On a macroscopic level, looking at the overall organism, there must certainly be a range of efficiency when measuring incident photons relative to carbon fixation.
Jun
11
comment Does the use of “var”, “x”, and/or “ssp” in a scientific name provide specific information?
Ah, thanks! Simple typo. ;)