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Oct
20
comment What is the difference between orthologs, paralogs and homologs?
I modified your answer to be less forceful in its statement that terdon took issue with. If you don't agree with it, you can reject the change.
Oct
20
revised What is the difference between orthologs, paralogs and homologs?
Edited statement in accordance with commends below. Clarified grammar.
Oct
20
comment Why do drugs which are hormones have long half-life?
This is a good, broad answer to the OP's question. I would add, for protein hormones at least, they tend to be secreted as autocrine/paracrine/endocrine hormones, and by definition need only carry a signal for a brief period of time before the hormone is degraded (otherwise signalling would go on for longer than is necessary). For example, insulin has a half-life of on the order of minutes.
Oct
19
comment Why do drugs which are hormones have long half-life?
Peptide hormones have short half-lives because there are enzymes in blood plasma to degrade them. Half-life of any substance depends on, among other things, their route of metabolism and delivery.
Oct
19
comment Can I image Coomassie and GFP in gels at the same time with a fluorescence scanner?
Can you explain how you get actual fluorescence images from your gels in which GFP is denatured?
Oct
17
awarded  Excavator
Oct
17
comment How many human proteins have a solved 3D structure?
This is a very subject question for two reasons: The Protein Databank accepts NMR and crystal structures of proteins which offer different degrees of resolution and accuracy. These structures are also somewhat subjective because the protein may adopt different conformations depending its relevant biological context. Thirdly, the solvent extractions may impose erroneous structure.
Oct
17
revised What effect has changing pH and salt concentration on protein complexes?
Fixed typo.
Oct
17
accepted Finding and Comparing Homologous Genes
Oct
17
comment Are there any websites offering graphs for the light absorption of different enzymes?
@rwst - This is true, and is only possible if an in vitro assay for enzyme activity exists. It also relies on the purified enzyme being active, which is not always the case. The question asked was more broadly stated.
Oct
17
comment Are there any websites offering graphs for the light absorption of different enzymes?
@AbdelrahmanEsmat - they don't need to know specific information about the enzyme (or any protein's) absorbance because it is inferred based on knowledge of how amino acids absorb light at specific wavelengths.
Oct
17
comment Genetic engineering for insulin production
@Bitwise, January - I stand corrected. I meant to say the modifications from eukaryotic cells is different. :)
Oct
16
awarded  Custodian
Oct
16
comment Genetic engineering for insulin production
You may like to read this link. It provides a lay explanation for your exact question. littletree.com.au/dna.htm
Oct
16
comment Genetic engineering for insulin production
Also, insulin is an uncommon protein in that it does not get post-translationally modified by glycosylations, phosphorylations, etc. These modification mechanisms do not exist in bacteria. This is why bacteria are used for biotech protein production.
Oct
16
revised Are there any websites offering graphs for the light absorption of different enzymes?
Typo; further explanation as per OP's clarification
Oct
16
answered Are there any websites offering graphs for the light absorption of different enzymes?
Oct
10
comment Multi-nucleated cells: advantages and examples?
That's an example I was not aware of, but very interesting.
Oct
8
comment Does an annealing temp higher than primer's Tm contribute to primer dimer?
You can always use a lower Tm, but then you sacrifice specificity. In situations like these, I like using a gradient thermocycler to test a range of annealing temperatures to find the optimum.
Oct
8
awarded  Custodian