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Apr
20
revised What is the highest competency possible for E coli?
Corrected a formattin error, and more clearly stated a direct answer.
Apr
20
answered What is the highest competency possible for E coli?
Apr
19
comment Why are restriction enzymes not frozen?
Glycerol is added as an anti-freeze agent as well, so that the enzyme can be kept at low temperature (low kinetic energy) and therefore stay stable for longer periods of time.
Apr
18
comment What exactly are computers used for in DNA sequencing?
@KonradRudolph - in that case, the fewer, but longer reads will make computation time faster?
Apr
18
answered What is the reason behind choosing the reporter gene when experimenting on your gene of interest?
Apr
18
accepted When collecting cell lysates for a Western blot, how do I induce di-sulfide bonds?
Apr
18
accepted Where do the bacteria within the vagina originate from?
Apr
18
comment What exactly are computers used for in DNA sequencing?
This is a very good question. Is the computation easier if you have 300,000,000 reads of 100 bp length, or 60,000,000 reads of 500 bp in length?
Apr
18
answered Protein Biology Cheat Sheet
Apr
17
answered Measuring reverse transcriptase activity in E. coli
Apr
16
answered What's the aim of genetically modifying of foods/organisms?
Apr
16
comment Dimensionless number for blood volume
It is perfectly valid to make normalized comparisons that have dimensions. I think the stride length being dimensionless was coincidental (body length/stride length). An example measurement that normalizes body parameters is the Body Mass Index (BMI) which scales mass:height^2, or the fuel economy of cars express as miles per gallon.
Apr
16
comment Why are there N's after Sanger sequencing?
I'll add on to this answer. A Sanger sequence is really only good for (at best) 800-900 bp. After this time, there is simply too little DNA left to have a reliable signal that you can confidently say is a specific nucleotide. A good habit to get into is always examine your chromatograms and trace, and reject everything after you start getting your first run of N's. I don't usually worry about my first N at the end of the read however because it can be attributed just to error, and the following bases are still confidently called.
Apr
15
comment Neutralizing TCA washes
From the MSDS for TCA, it states to neutralize with sodium bicarbonate so as to prevent Cl2 gas formation (like with neutralization with HCl).
Apr
15
comment Do probiotics survive digestion?
@Danielle - I gave Ilmari the accepted answer since it was better researched.
Apr
15
accepted Do probiotics survive digestion?
Apr
15
comment Do probiotics survive digestion?
This is interesting that some species are viable and others are not. Thank you for the quote.
Apr
15
answered Online Molecular and Cellular Biology Video Lectures?
Apr
14
revised PCR primer in highly repetitive region
added 266 characters in body
Apr
14
answered PCR primer in highly repetitive region