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Oct
17
comment Are there any websites offering graphs for the light absorption of different enzymes?
@AbdelrahmanEsmat - they don't need to know specific information about the enzyme (or any protein's) absorbance because it is inferred based on knowledge of how amino acids absorb light at specific wavelengths.
Oct
17
comment Genetic engineering for insulin production
@Bitwise, January - I stand corrected. I meant to say the modifications from eukaryotic cells is different. :)
Oct
16
awarded  Custodian
Oct
16
comment Genetic engineering for insulin production
You may like to read this link. It provides a lay explanation for your exact question. littletree.com.au/dna.htm
Oct
16
comment Genetic engineering for insulin production
Also, insulin is an uncommon protein in that it does not get post-translationally modified by glycosylations, phosphorylations, etc. These modification mechanisms do not exist in bacteria. This is why bacteria are used for biotech protein production.
Oct
16
revised Are there any websites offering graphs for the light absorption of different enzymes?
Typo; further explanation as per OP's clarification
Oct
16
answered Are there any websites offering graphs for the light absorption of different enzymes?
Oct
10
comment Multi-nucleated cells: advantages and examples?
That's an example I was not aware of, but very interesting.
Oct
8
comment Does an annealing temp higher than primer's Tm contribute to primer dimer?
You can always use a lower Tm, but then you sacrifice specificity. In situations like these, I like using a gradient thermocycler to test a range of annealing temperatures to find the optimum.
Oct
8
awarded  Custodian
Oct
8
reviewed Reviewed What gaseous substances do humans emit?
Oct
8
answered Multi-nucleated cells: advantages and examples?
Oct
7
answered Where are -HCl supplements absorbed in the human digestive system?
Oct
1
comment Do Yeast Insertion constructs revert?
Disclaimer: I only work with bacteria. This seems analogous to antibiotic selection in E. coli, and as such, selection pressure is needed to maintain the engineered yeast population to be assured that you aren't incubating environmental strains.
Sep
21
awarded  Custodian
Sep
20
awarded  Enlightened
Sep
20
awarded  Nice Answer
Sep
13
awarded  Nice Question
Sep
12
comment How are DNA polymerase error rates measured?
Thank you for the reference. This seems like the most pragmatic approach to this problem. Even though by design this method under-reports the error rate, it would be a consistent bias across polymerases.
Sep
12
accepted How are DNA polymerase error rates measured?