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Jan
10
comment Cardiac ECG database with high sampling rate?
How were you searching Pubmed? There are 30,000+ results for "Holter" and that can be refined further to include sampling frequency, etc. Also, the 12-lead EKG's are capable of 4096 Hz resolution according to this paper: plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0081281
Jan
4
comment Shine-Dalgarno sequence and expressing proteins
In this case a simple PCR with the 5' oligonucleotide altered to change the SD sequence. Alternatively, mutagenesis PCR can be used if starting from a circular plasmid ready for expression.
Jan
2
answered Shine-Dalgarno sequence and expressing proteins
Dec
29
comment ligand binding fluorescent protein
You can try looking for fluorescent sensors. These schemes sense (bind to) certain ions to increase fluorescence. BiFC (bi-fluorescence complementation) is a way to see fluorescence increase upon binding of two protein entities.
Dec
14
comment Why is cyanide poisonous?
Electrons get stuck because they can't be passed to complex IV, and this causes a backup in all the complexes of the electron transport chain. Therefore the cell faces an energy crisis, and eventually causes death.
Dec
9
comment Why is Thermus aquaticus and Taq Pol stable?
While I doubt that oligomerization is used to stabilize Taq DNA Pol, oligomerization (usually through C- or N-terminal tails) can help stabilize larger, quaternary, structure. For example: hemoglobin, insulin.
Dec
8
answered Why is Thermus aquaticus and Taq Pol stable?
Dec
6
reviewed Close Organisms with the most extreme sensory capabilities? (sight, smell, sound)
Dec
4
comment Why do yeast ferment Equal sweetener
Alan, quite right. I made a mistake. The open chain form is in equilibrium with alpha and beta cyclic pyranose and furanose forms. I have corrected myself.
Dec
4
revised Why do yeast ferment Equal sweetener
Corrected a context error.
Dec
3
answered Why do yeast ferment Equal sweetener
Dec
1
comment Left brain vs right brain
As far as I know, this is cute, but false, dichotomy in neuroscience. The two sides of brain work together for proper function.
Dec
1
reviewed Close Protein Structure Statistics
Dec
1
reviewed Approve suggested edit on paralogue genes in genome-wide association studies?
Dec
1
reviewed Approve suggested edit on Do invasive species cause long-term damage to ecosystems they invade?
Dec
1
comment The reason why researchers usually use cell lines from “blast cells”?
It's probably because those are the cells they have access too, or they're trying to figure out expression levels in some differentiated lineage. I've seen lots of expression kinetic experiments done in bacteria, yeast, and all sorts of standard mammalian cell lines.
Nov
30
reviewed Approve suggested edit on microbiology tag wiki
Nov
30
reviewed Close Interdependency between organsims on our planet and its relation to ecosystem?
Nov
29
comment Assay for Beta-galactosidase activity in single cell microscopy
That's true about imaging at 60x, but it requires cells to be well separated for individual detection, and that's not usually the case with bacteria, especially when actively growing. That said, the resolution requirements were a big ambiguous. :) If you're using a microfuidic device, the sugar gets washed in with the bulk flow, so wouldn't that occur on a much faster time scale than the uptake and metabolism in bacteria?
Nov
29
answered Assay for Beta-galactosidase activity in single cell microscopy