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| bio | website | guizzetti.ca |
|---|---|---|
| location | London, Ontario | |
| age | 26 | |
| visits | member for | 1 year, 2 months |
| seen | 10 hours ago | |
| stats | profile views | 27 |
I'm Leo and I'm passionately involved in biology and running, and interested in programming and cooking.
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Dec 24 |
reviewed | Close What are studies linking dietary choices over life with probability of reaching higher ages? |
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Dec 24 |
answered | Why can't you taste food when you have a cold? |
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Dec 23 |
revised |
HIV Rapid Tests added 2 characters in body |
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Dec 23 |
reviewed | No Action Needed HIV Rapid Tests |
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Dec 20 |
comment |
Can paper/plastic currency serve as a medium for pathogens? The same may be said for any of the pathogenic E. coli which can persist on surfaces (including plastic) for a day or two. |
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Dec 17 |
comment |
Proteins that are strongly overproduced in E. coli and S. cerevisiae? hehe, just listed a promoter since it's what was on my mind at the time. ;) |
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Dec 16 |
reviewed | No Action Needed Where might I find a list of all recorded instincts by species? |
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Dec 15 |
comment |
Proteins that are strongly overproduced in E. coli and S. cerevisiae? I think this comment (with respect to E. coli) was addressed here (biology.stackexchange.com/questions/5197/…). If you're looking to maximize plasmid protein expression, then you'll want a highly active constitutive promoter (eg, CMV), and then depending on codon optimization and many other factors, you might get ~20% by weight of your protein. |
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Dec 12 |
answered | Bacterial cell lysis - what solution to use? |
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Dec 10 |
comment |
Gene & Protein nomenclature: N-Myc, c-Myc, et. al I was being polite. ;) Which genes are those? |
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Dec 10 |
answered | Gene & Protein nomenclature: N-Myc, c-Myc, et. al |
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Dec 6 |
comment |
Low temperature PCR Doesn't this accomplish the same thing as DMSO? |
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Dec 6 |
revised |
Can a human smell through the ears? added 283 characters in body |
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Dec 6 |
comment |
Can a human smell through the ears? Exactly. Just as it's impossible for eyes to perceive sound, or to smell colours. |
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Dec 6 |
answered | Can a human smell through the ears? |
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Dec 3 |
answered | How does ethanol interact with insulin? |
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Dec 3 |
revised |
How does ethanol interact with insulin? deleted 8 characters in body; edited tags |
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Dec 2 |
reviewed | Reviewed Will a completely removed cat whisker grow back? |
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Dec 2 |
reviewed | Reviewed How much energy does human body fat contain? |
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Dec 1 |
comment |
What are the advantages and disadvantages of using beta-galactosidase compared to luciferase as a reporter gene? @AlanBoyd the induction of lac is irrelevant to the discussion, but I've included it for completeness. IPTG is only required if lacZ repressor is overexpressed. For example, blue/white screening is easily achieved with X-gal alone for DH10B (TOP10) strains, Mach-T1, DH5A-T1, but not others (see page viii: tools.invitrogen.com/content/sfs/manuals/topota_man.pdf ). |
