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May
5
comment What is the difference between transformation and transfection?
To add on with how the words are currently used, bacteria undergo transformation, while introducing plasmids to cell lines is called transfection (often incorrectly, since most people don't use live virus for infection, but use cationic lipids instead).
May
5
comment If not intelligent design, what is an alternative scientific theory to evolution?
Again, you have a misconception that hypotheses need to be tested in a competitive fashion. There were alternatives to Darwin's evolution, see Lamarckism. The reason evolution has lasted so long, and was promoted from hypothesis to theory, is because it works extraordinarily well in many circumstances. For the aspects that Darwin could not have known about (e.g., epigenetics), the theory has been improved, or "upgraded", to account for the current body of evidence.
May
4
reviewed Close About virus developement
May
4
reviewed Edit suggested edit on How can toddlers expend seemingly boundless energy when they eat so little?
May
4
revised How can toddlers expend seemingly boundless energy when they eat so little?
small formatting changes; and improved grammar
May
4
comment Why is weight gain a symptom of Cushing's Syndrome?
Could you provide a more detailed answer of this, preferably with a reference?
May
4
reviewed Close Ammonia smell after physical exercise? What is the explanation?
May
4
comment How to calculate virus titre from qPCR
I'm still not clear why you are trying to titre viral pfu using the manufacturing system (HEK293T cells) rather than your intended experimental system? Infectivity is certainly a function of host cell, but only makes sense in your experimental system. I guess my question is, why is a plaque assay system that difficult to use? Perhaps I have not understood some nuance of your particular system.
May
3
answered How to calculate virus titre from qPCR
May
3
comment Are there special constants in biology that define organism's morphology?
There are 20 references concerning facial symmetry and the golden ratio indexed on PubMed, and nearly 800 mentioning the golden ratio itself. I'm not sure there will be scientific evidence per se, since that would require experimental evidence. Instead, most of what I've seen about the golden ratio (or the associated logarithmic spiral or golden angle) are observational reports. The significance of this number is debated, but I don't see any arguments discounting its existence.
May
2
accepted Dimerization of Immunoglobulin G
May
2
comment Dimerization of Immunoglobulin G
What's the nature of the hydrogen bonds/non-covalent bonding between two heavy chains? Are they naturally aggregated in the cell?
May
2
comment Dimerization of Immunoglobulin G
No, of course each immunoglubulin-like domain has an intramolecular disulfide (stabilizing the beta sandwich conformation). I mean between two heavy chain molecules.
May
2
answered Are there special constants in biology that define organism's morphology?
May
2
asked Dimerization of Immunoglobulin G
Apr
29
reviewed Approve suggested edit on How does laughing gas (N2O) work?
Apr
29
accepted Polarized epithelium and localization of ion channels
Apr
29
revised conservation of C-peptide sequence in the guinea pig
Expanded my answer and added a reference.
Apr
29
answered conservation of C-peptide sequence in the guinea pig
Apr
28
reviewed Approve suggested edit on Why do so many people have group O blood?