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comment What is accumulation and release centre of neurohormones?
There are many tissues expressing and secreting neuropeptides and hormones. Certainly the hypothalamus and pituitary gland, but there are clusters of enervating through the brsinstem secreting GLP1, a host of enteroendocrine tissues to name a few. Are there some hormones in particular you are interested in?
2d
comment Could someone Identify this Insect Hive on my Balcony Grill
I suggest killing it with fire. Paper wasps in North America can be very aggressive and their stings hurt.
2d
comment Speed of effective lymphatic drainage?
I'm sorry I really don't remember.
2d
comment Does our eyeball increase in size as we grow?
Hi Ilan that comment comes across as quite terse, even rude. Such behaviour is not conducive to good discussions on this website and I recommend using constructive criticism in future if you have an issue with an answer. For example, your post is equally without citation regarding growth of the eye (not lens). Let's try to get along.
2d
comment Speed of effective lymphatic drainage?
Looks correct, Masi.
2d
comment Speed of effective lymphatic drainage?
I vaguely recall an old study looking at lymphatic flow in sheep, and the range being very heterogeneous (virtually no flow at the lower bound). I would agree that, in general, this is a very heterogeneous effect subject to many chemical (hydration, osmotic balance, blood pressure modifying drugs) and physical (cardiovascular tone, posture, activity level) factors.
Apr
14
comment Is Diabetes sex linked disease
I'm not aware of any of the genes implicated in either T1DM or T2DM being sex-linked. However, there is maturity-onset diabetes in the young (MODY, for short). MODY is a subset of T2DM that are caused by an autosomal dominant mutation in one of a few genes (e.g., glucokinase, KCNJ11, ABCC8, INS). These are all rare, heritable forms of T2DM with various responses to traditional T2DM therapies.
Apr
14
comment enzymes that stabilize DNA loops
With specific attention to more efficient ligation (e.g., after restriction enzyme digestion), there are commercial products on the market that use a modified topoisomerase to catalyze such a ligation.
Apr
12
comment Troubleshooting for Agarose Gel Electrophoresis
I tend to agree with this answer. Another cause for exceeding the max current could be a result of both high percentage gel in combination with old buffer, each contributing to greater electrical resistance. I prefer not running the voltage too fast otherwise you lose resolution due to the additional heat that causes the superficial gel surface to melt, appearing blurry.
Apr
2
comment How do I get a brighter DNA bands
The blue LED transillumination is the problem. There is nothing wrong with making your gel, electrophoresis or resolution of bands based on your image. EtBr is best illuminated in the short UV range and is a function of wavelength. It's worth comparing abaorbance ratio for peak UV illumination vs the wavelength of blue LED (415 nm? ). 20 sec exposure is awfully long. What do 100 ms to 1 sec exposures look like?
Apr
2
comment How do I get a brighter DNA bands
Standard amounts are about 16 ul per 100 ml of gel IF added in the gel solution.
Mar
31
comment Nervous System : Organ or Gland Failure
Pancreatic islets also have vagal innervations, though arguably the nervous control is not the primary control mechanism, the PNS/SNS certainly can exert extensive control over them.
Mar
27
comment Concentration of DNA by isopropanol
Not to mention every molecular bio lab has isopropanol on hand.
Mar
17
comment Which chemical and hormonal effects on heart are by metabolites?
Strictly speaking, hormones are either steroid derivatives or peptides, which transduce their effect in specific ways (e.g., diffusion into the cell to bind a target in the case of steroids, or signalling through a surface receptor for peptides). Catecholamines are neurotransmitters and not considered hormones by the classical definition. The effects can either be autocrine, paracrine or endocrine depending on the distance of action. Ions will typically affect the electrical conductivity of the myocardium and can be considered passive in nature, but ions are not metabolites.
Mar
5
comment Plasmid maintenance
I'll add to this as well. Always keep a small aliquot of your miniprep DNA in a stock box, and keep it frozen, just in case the glycerol stock fails for some reason (e.g., freezer failure). The DNA is more forgiving of freeze-thaws and offers one more backup. I only do this with final (or destination) vectors that I have finished manipulating.
Mar
3
comment How to avoid air bubbles while pipetting?
Sometimes with very viscous liquids (such as detergent solutions), it may be necessary to slowly "rinse" the inside of the pipette by doing a full pieptte up and down of the liquid before an accurate amount will be drawn.
Mar
1
comment Promoter in the lac operon
I'm inclined to agree with this answer. I know that the distinction between the lac operon being a distinct domain from the lac operon has been known since at least the early 1970s. The lac promotor/operon regulatory region is also known to be leaky, suggesting Alan's answer is correct.
Feb
27
comment What is immunosuppression? Why would one use it?
This question appears to be off-topic because the answer is readily available by simple web search.
Jan
10
comment Cardiac ECG database with high sampling rate?
What did you search on PubMed? If you search "holter molitor" and filter by review there are ~900 results.
Jan
10
comment Cardiac ECG database with high sampling rate?
How were you searching Pubmed? There are 30,000+ results for "Holter" and that can be refined further to include sampling frequency, etc. Also, the 12-lead EKG's are capable of 4096 Hz resolution according to this paper: plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0081281