network profile
| bio | website | |
|---|---|---|
| location | London, United Kingdom | |
| age | 27 | |
| visits | member for | 1 year, 2 months |
| seen | Dec 1 '12 at 12:16 | |
| stats | profile views | 3 |
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Oct 10 |
comment |
Ammonium sulphate precipitation assay pH dependence the protein isn't hydrophobic, it is the ammonium's precipitating tendency as in the Hoffmeister series. I HAVE to use an ammonium salt, so the hoffmeister series cannot help me in that respect |
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Oct 10 |
accepted | Ammonium sulphate precipitation assay pH dependence |
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Oct 9 |
comment |
Ammonium sulphate precipitation assay pH dependence Thanks. I'm actually trying to stop precipitation when incubating a protein in ammonium acetate, and I wondered if an acidic pH would reduce this. Do you have any suggestions |
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Oct 8 |
asked | Ammonium sulphate precipitation assay pH dependence |
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Mar 15 |
awarded | Scholar |
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Mar 15 |
accepted | Is there a binding affinity metric for interactions not in equilibria? |
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Mar 4 |
awarded | Supporter |
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Mar 2 |
comment |
Is there a binding affinity metric for interactions not in equilibria? I don't think this is right because, k-on/k-off = Ka and Ka=1/Kd. So that would mean having K-on and K-off would allow you to calculate Kd, which is an equilibrium measurement... ah well thanks for trying! |
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Mar 1 |
awarded | Student |
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Mar 1 |
asked | Is there a binding affinity metric for interactions not in equilibria? |
