160 reputation
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location London, United Kingdom
age 29
visits member for 2 years, 8 months
seen Oct 15 at 17:00

Oct
10
comment Ammonium sulphate precipitation assay pH dependence
the protein isn't hydrophobic, it is the ammonium's precipitating tendency as in the Hoffmeister series. I HAVE to use an ammonium salt, so the hoffmeister series cannot help me in that respect
Oct
10
accepted Ammonium sulphate precipitation assay pH dependence
Oct
9
comment Ammonium sulphate precipitation assay pH dependence
Thanks. I'm actually trying to stop precipitation when incubating a protein in ammonium acetate, and I wondered if an acidic pH would reduce this. Do you have any suggestions
Oct
8
asked Ammonium sulphate precipitation assay pH dependence
Mar
15
awarded  Scholar
Mar
15
accepted Is there a binding affinity metric for interactions not in equilibria?
Mar
4
awarded  Supporter
Mar
2
comment Is there a binding affinity metric for interactions not in equilibria?
I don't think this is right because, k-on/k-off = Ka and Ka=1/Kd. So that would mean having K-on and K-off would allow you to calculate Kd, which is an equilibrium measurement... ah well thanks for trying!
Mar
1
awarded  Student
Mar
1
asked Is there a binding affinity metric for interactions not in equilibria?