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comment What limits the speed of human perception?
Please don't use MathJax for regular text formatting. It is much slower and should only be used when necessary, for math and chemical formulas.
Jul
23
comment How many different protein coding genes are in the Human Biome?
The main text of your answer is entirely copied from a Wikipedia article. While you mentioned the source at the end of your post, you didn't mark the text as a quote. This is still plagiarism and not allowed on this site. You have to make clear any place where you quote a source verbatim. Answers should also not be taken entirely from external sources, they should be in your own words and quotes should be only for support.
Jul
23
comment Is it possible to genetically modify a plant at home?
You might also have to consider any regulations that might exist in your country. In some countries genetic modified organisms can only be handled in designated laboratories.
Jul
20
comment Question about the DNA glycosylase enzyme assay/formamide gel loading buffer
Formamide is a common loading buffer for denaturing gel electrophoresis of nucleic acids. I'd assume that it also denatures most enzymes and stops the reaction that way.
Jul
18
comment Where are the evolutionary “inbetweeners”?
I'm closing this according to our policy on creationist questions. If you're interesting in understanding evolution you're welcome to ask here. Questions that simply state the common creationist talking points will be closed.
Jul
15
comment Remove RNAse from pipettes?
I'd also switch out all ingredients for your in vitro transcription if you have any RNAse problem. You might also want to aliquot the stocks further, to limit the damage contamination causes, and to protect the more sensitive stocks. Some stuff just gets bad quicker, DTT should be reasonably fresh, and NTPs also get noticeably worse if they're older. But we also don't use kits but prepare all the stuff in our lab.
Jul
14
comment Remove RNAse from pipettes?
I'd disassemble the pipettes and put the appropriate parts in DEPC solution. I would also consider other issues aside from RNAses, reduced yield can have many different causes.
Jul
2
awarded  Curious
Jun
26
revised What Proteins Are Universal To All Life Forms?
added 202 characters in body
Jun
21
comment How can cells produce antibodies despite error-checking mechanisms in their genomes?
This process is called somatic hypermutation. I just looked it up in my Alberts and it seems the exact mechanism is not known (though the book is a bit older now, this might have changed).
Jun
20
comment Ribosomal Turing Machines, DNA/RNA computation
There are some papers on simple computations using nucleic acids, if nobody answers I'll look them up later. As for your examples, nature already does some of them (see RNA interference). And for correcting genetic diseases, the hard part is actually correcting the sequence in all cells in your body, not the detection itself.
Jun
16
comment What I can do in order to improve the folding of the protein?
You'll have to add a lot more detail if you want to have a chance for any proper advice. All the known properties of the protein as well as your exact expression and purification protocols at minimum. Otherwise you'll only get some guesses and vague advice.
Jun
14
reviewed Approve suggested edit on What makes DNA helical?
Jun
12
comment How does one quantify T7 RNA polymerase transcription efficiency?
Do you want to measure how much RNA the transcription produced, or do you want to predict the efficiency? Because in vitro transcription efficiency depends a lot on the sequence and the exact conditions, and is hardly predictable.
Jun
11
comment Local storage of NCBI database in MongoDB
@shigeta I usually put the line pretty far on the informatics side when deciding whether a question is off-topic, but this question is a bit far on the informatics side for me. The main question, how to convert an SQL database to MongoDB is better asked on a specialized site. But there is a question below that that might require some biology knowledge, and that is whether a NoSQL approach is useful for an SNP database.
Jun
11
comment How do penicillin resistant bacteria grow slower in the presence of penicillin?
You need to add a lot more detail for any answer to be possible. Which kind of bacteria were in there? Were they resistant to Penicillin. Did you work sterile?
Jun
11
comment What is the best file format to store gel images?
@terdon The lossless compression of PNGs still results in much larger images than JPEG. I personally use PNGs for almost everything, but there are situations where you need smaller files and where JPEG can be acceptable.
Jun
11
revised What is the best file format to store gel images?
deleted 1 character in body
Jun
11
comment What is the best file format to store gel images?
@Chris I think the major issue here is not TeX-specific, it is more about good practices for documentation of scientific data.
Jun
11
answered What is the best file format to store gel images?