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May
5
comment Biopython - Big Discrepancy Calculating RNA melting Temperature over Literature
The source code for that function is available on Github, there are also some comments in there explaining what methods Biopython uses. The issue might also be that you're comparing apples and oranges, and that your siRNA Tm are not for 7mer parts of an siRNA but something else.
Apr
29
reviewed Reject NADH vs. NADPH: Where is each one used and why that instead of the other?
Apr
15
awarded  Nice Question
Mar
26
revised Change solvent of bacterial solution
added 41 characters in body
Mar
26
answered Change solvent of bacterial solution
Mar
23
awarded  Enlightened
Mar
23
awarded  Nice Answer
Mar
17
comment Proteins in Milk, Oat , Eggs and Soy
@MARamezani proteins are biomolecules, allergies are a biological mechanism, why would this be better on Chemistry than Biology?
Mar
12
revised Is there a specific mechanism for the delivery of pain medication?
edited tags
Mar
2
comment How do I identify the protein with the highest Disulfide bond density? i.e protein with highest ratio of Disulphide bonds per Peptide bond?
You can download all sequences from the PDB in one file: (gzipped fasta format)
Feb
26
comment The human organism knows what we have to eat?
@ChrisStronks Please don't recommend Skeptics unless you explain the requirements of the site. Especially the notability rules, which otherwise tend to get most questions by new users closed.
Feb
24
revised Can cell exist without Ribosomes?
appended answer 29889 as supplemental
Feb
19
awarded  Informed
Feb
16
revised How does it make thermodynamic sense for photosynthesizers to turn CO₂ into O₂?
deleted 10 characters in body
Feb
7
comment What is the fruit of Café marron (Ramosmania rodriguesi) like?
This is really a cooking question, and not a biology question.
Feb
4
reviewed Reject Are there U-rich transcriptional pause sites?
Feb
3
revised How do biologists infer correct ORF of a DNA sequence?
added 6 characters in body; edited title
Jan
27
comment Can genetic engineering be performed without a laboratory setting?
I think you're overestimating the difficulty of buying cells and chemicals, I'd guess that registering a business might be enough to fool suppliers. But the other arguments are valid, you're likely to run into significant walls trying to do anything useful at home. Even with second hand equipment, a basic lab would still be a considerable expense.
Jan
27
comment Can genetic engineering be performed without a laboratory setting?
I'd make the warning about phenol/chloroform more explicit, doing that extraction outside of a fume hood is a pretty bad idea. Phenol is pretty toxic and not something I'd use in home experiments.
Jan
27
comment Can genetic engineering be performed without a laboratory setting?
You'll have to be careful about the local laws, in some countries you need a laboratory with permission for handling GMOs, doing such work outside it would be illegal.