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Jan
22
asked Why are riboswitches mostly present in bacteria and not in eukaryotes?
Jan
20
comment Are there any enzymes compatible with uracil, thymine, adenine, guanine, or cytosine that we know of?
At this moment your question doesn't really make sense. Thymine doesn't pair with Uracil, and the whole concept of base-pairing doesn't really apply to enzymes. If you clarify your question, we can reopen it, but as it is now we would have to guess what you mean.
Jan
11
answered What are the requirements for a spectrometer to be used in biological research?
Dec
30
revised Why isn't the insertion of a single nucleotide destructive for DNA?
edited tags
Dec
30
answered Why isn't the insertion of a single nucleotide destructive for DNA?
Dec
29
comment Why is mRNA needed in the Protein translation?
@user137 you can perform many translations from a single mRNA. So you don't need to transcribe the DNA into mRNA as often as you translate the mRNA into protein.
Dec
29
comment Why is mRNA needed in the Protein translation?
@inf3rno I was thinking about including the fact that multiple ribosomes can read the same mRNA at the same time, but decided to skip that to avoid making it more complicated than necessary. And one codon distance is certainly not possible, the ribosome is quite large.
Dec
29
answered Why is mRNA needed in the Protein translation?
Dec
18
comment How can I purify RNA after gel electrophoresis to remove residual acrylamide?
@user137 The RNA you would investigate with NMR is usually around 15-150 nt long, you don't see much with NMR for anything larger. And we simply do the in vitro transcriptions on a larger scale, typically around 10-30 ml. RNA is also almost always measured in Shigemi tubes, so the volume is 300 ul, and while 1 mM concentration is ideal, but you can't always get that much.
Dec
18
comment How can I purify RNA after gel electrophoresis to remove residual acrylamide?
@user137 Depending on what exactly you want to do you need about 0.2-1.0 mM protein or RNA in your sample in a volume of 300-500 microliters. And for many experiments you also need it 13C and/or 15N-labeled.
Dec
18
awarded  Enlightened
Dec
18
awarded  Nice Answer
Dec
16
reviewed Leave Open Use cases of RNA secondary structure prediction
Dec
16
comment Use cases of RNA secondary structure prediction
@WYSIWYG I don't think broadness is a problem here. A detailed review of structural elements is not required for an answer, just an explanation why RNA secondary structure is important to know (and to some degree why RNA is important).
Dec
16
awarded  Notable Question
Dec
16
comment Use cases of RNA secondary structure prediction
Another thing, you mention only mRNA translation. Riboswitches can act at transcription or translation, transcriptional riboswitches typically terminate the transcription early to regulate, translational riboswitches typically sequester the Shine-Dalgarno sequence to regulate expression.
Dec
16
answered Use cases of RNA secondary structure prediction
Dec
16
comment Use cases of RNA secondary structure prediction
rcsb.org/pdb/101/motm.do?momID=130
Dec
16
comment Use cases of RNA secondary structure prediction
Huh, where did you get the idea that it is impossible to get the full 3D structure of a riboswitch? There are structures published for most of the known classes of riboswitches.
Dec
14
awarded  Yearling