5,539 reputation
32448
bio website
location
age
visits member for 3 years
seen 35 mins ago

Dec
18
comment How can I purify RNA after gel electrophoresis to remove residual acrylamide?
@user137 The RNA you would investigate with NMR is usually around 15-150 nt long, you don't see much with NMR for anything larger. And we simply do the in vitro transcriptions on a larger scale, typically around 10-30 ml. RNA is also almost always measured in Shigemi tubes, so the volume is 300 ul, and while 1 mM concentration is ideal, but you can't always get that much.
Dec
18
comment How can I purify RNA after gel electrophoresis to remove residual acrylamide?
@user137 Depending on what exactly you want to do you need about 0.2-1.0 mM protein or RNA in your sample in a volume of 300-500 microliters. And for many experiments you also need it 13C and/or 15N-labeled.
Dec
18
awarded  Enlightened
Dec
18
awarded  Nice Answer
Dec
16
reviewed Leave Open Use cases of RNA secondary structure prediction
Dec
16
comment Use cases of RNA secondary structure prediction
@WYSIWYG I don't think broadness is a problem here. A detailed review of structural elements is not required for an answer, just an explanation why RNA secondary structure is important to know (and to some degree why RNA is important).
Dec
16
awarded  Notable Question
Dec
16
comment Use cases of RNA secondary structure prediction
Another thing, you mention only mRNA translation. Riboswitches can act at transcription or translation, transcriptional riboswitches typically terminate the transcription early to regulate, translational riboswitches typically sequester the Shine-Dalgarno sequence to regulate expression.
Dec
16
answered Use cases of RNA secondary structure prediction
Dec
16
comment Use cases of RNA secondary structure prediction
rcsb.org/pdb/101/motm.do?momID=130
Dec
16
comment Use cases of RNA secondary structure prediction
Huh, where did you get the idea that it is impossible to get the full 3D structure of a riboswitch? There are structures published for most of the known classes of riboswitches.
Dec
14
awarded  Yearling
Dec
11
comment Why deoxyribose for DNA and ribose for RNA?
As far as I know, the hydrolysis of RNA via the 2'OH doesn't play any role in regulating mRNA levels, there are lots of active RNA degradation mechanisms that are responsible for that.
Dec
11
comment Why deoxyribose for DNA and ribose for RNA?
Do you have a reference for the claim that the 2'OH is important for regulating mRNA? My intuitive guess would be that active degradation by RNAses would be far more important than the chemical instability by the 2'OH.
Dec
10
revised Should I dilute DNA with water or elution buffer?
edited body; edited tags; edited title
Dec
10
answered Should I dilute DNA with water or elution buffer?
Dec
8
revised How does the cornea breathe during sleep?
added 7 characters in body; edited tags; edited title
Nov
25
revised Can an organism process H₂O into H₂O₂?
deleted 1 character in body
Nov
24
awarded  Enlightened
Nov
24
awarded  Nice Answer