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Feb
24
revised Can cell exist without Ribosomes?
appended answer 29889 as supplemental
Feb
19
awarded  Informed
Feb
16
revised How does it make thermodynamic sense for photosynthesizers to turn CO₂ into O₂?
deleted 10 characters in body
Feb
7
comment What is the fruit of Café marron (Ramosmania rodriguesi) like?
This is really a cooking question, and not a biology question.
Feb
4
reviewed Reject Are there U-rich transcriptional pause sites?
Feb
3
revised How do biologists infer correct ORF of a DNA sequence?
added 6 characters in body; edited title
Jan
27
comment Can genetic engineering be performed without a laboratory setting?
I think you're overestimating the difficulty of buying cells and chemicals, I'd guess that registering a business might be enough to fool suppliers. But the other arguments are valid, you're likely to run into significant walls trying to do anything useful at home. Even with second hand equipment, a basic lab would still be a considerable expense.
Jan
27
comment Can genetic engineering be performed without a laboratory setting?
I'd make the warning about phenol/chloroform more explicit, doing that extraction outside of a fume hood is a pretty bad idea. Phenol is pretty toxic and not something I'd use in home experiments.
Jan
27
comment Can genetic engineering be performed without a laboratory setting?
You'll have to be careful about the local laws, in some countries you need a laboratory with permission for handling GMOs, doing such work outside it would be illegal.
Jan
26
comment What are the differences between a benign tumor and swelling?
There is too much confusion of terms here for the question to be answerable.
Jan
22
asked Why are riboswitches mostly present in bacteria and not in eukaryotes?
Jan
11
answered What are the requirements for a spectrometer to be used in biological research?
Dec
30
revised Why isn't the insertion of a single nucleotide destructive for DNA?
edited tags
Dec
30
answered Why isn't the insertion of a single nucleotide destructive for DNA?
Dec
29
comment Why is mRNA needed in the Protein translation?
@user137 you can perform many translations from a single mRNA. So you don't need to transcribe the DNA into mRNA as often as you translate the mRNA into protein.
Dec
29
comment Why is mRNA needed in the Protein translation?
@inf3rno I was thinking about including the fact that multiple ribosomes can read the same mRNA at the same time, but decided to skip that to avoid making it more complicated than necessary. And one codon distance is certainly not possible, the ribosome is quite large.
Dec
29
answered Why is mRNA needed in the Protein translation?
Dec
18
comment How can I purify RNA after gel electrophoresis to remove residual acrylamide?
@user137 The RNA you would investigate with NMR is usually around 15-150 nt long, you don't see much with NMR for anything larger. And we simply do the in vitro transcriptions on a larger scale, typically around 10-30 ml. RNA is also almost always measured in Shigemi tubes, so the volume is 300 ul, and while 1 mM concentration is ideal, but you can't always get that much.
Dec
18
comment How can I purify RNA after gel electrophoresis to remove residual acrylamide?
@user137 Depending on what exactly you want to do you need about 0.2-1.0 mM protein or RNA in your sample in a volume of 300-500 microliters. And for many experiments you also need it 13C and/or 15N-labeled.
Dec
18
awarded  Enlightened