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Apr
13
reviewed Satisfactory In biosynthesis of cysteine and methionine, where does the sulfur come from?
Apr
13
reviewed Satisfactory What causes DNA to skew in gel electrophoresis?
Apr
13
reviewed Satisfactory Does antibacterial soap work chemically?
Apr
13
reviewed Needs Improvement eye myopia power changes with brightness
Apr
13
reviewed Satisfactory PCR enzyme units or concentration?
Apr
13
reviewed Needs Improvement Electronic cigarettes (e-cigs), any health risks?
Apr
9
comment How does GC-content evolve?
GC base pairs are more stable than AU/AT base pairs. I could imagine situations in which this plays a role, though I don't know if this is actually a relevant effect.
Apr
1
comment Is a sequential double transformation acceptable?
I'd assume that you use different antibiotic resistances for selection, that way you should be able to ensure that both plasmid are taken up successfully.
Mar
30
revised Substitute 25mM dNTPs mix with 10mM dNTPs
deleted 9 characters in body; edited title
Mar
28
revised Measuring reverse transcription yield?
edited body; edited title
Mar
27
revised Concentration of DNA by isopropanol
added 6 characters in body; edited title
Mar
25
awarded  Popular Question
Mar
18
comment Three great discoveries of modern science according to Gould?
This question is off-topic because it is not about biology, it is about what a specific person said at a specific event in the past.
Mar
11
comment What makes the amide bond the lowest energy pathway?
@MattDMo While this is a chemistry question, it is also about biologically important molecules, so it is still on-topic here.
Feb
28
revised parallels or replicates?
Biological or scientific terminology is on-topic, no need to mention that in the post.
Feb
28
answered How to avoid air bubbles while pipetting?
Feb
28
comment What is the significance of and biological mechanisms demonstrated in lac operon?
You'll have to be more specific about which aspects of the lac operon you don't understand, currently this question is far too broad.
Feb
24
comment RNase and EtOH issue
I'd probably clean the glass bottles with DEPC first to get rid of any RNAses. Usually baking at 200-250 °C is the way to go for glass, but the bottles typically have some plastic which would not survive this. I also tend to just reuse the same bottles for identical content, then you don't have to make them RNAse-free again.
Feb
24
answered RNase and EtOH issue
Feb
22
comment Why do doctors do chemo or transplantation for cancers that don't respond well to these treatments?
This is really a question about healthcare, not about biology and it is off-topic here.