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Feb
28
comment What is the significance of and biological mechanisms demonstrated in lac operon?
You'll have to be more specific about which aspects of the lac operon you don't understand, currently this question is far too broad.
Feb
24
comment RNase and EtOH issue
I'd probably clean the glass bottles with DEPC first to get rid of any RNAses. Usually baking at 200-250 °C is the way to go for glass, but the bottles typically have some plastic which would not survive this. I also tend to just reuse the same bottles for identical content, then you don't have to make them RNAse-free again.
Feb
24
answered RNase and EtOH issue
Feb
22
comment Why do doctors do chemo or transplantation for cancers that don't respond well to these treatments?
This is really a question about healthcare, not about biology and it is off-topic here.
Feb
16
accepted How can I fetch nucleotide sequences based on ENA accession numbers and positions?
Feb
16
revised What does 5' and 3' mean in DNA and RNA strands?
edited body; edited title
Feb
13
asked How can I fetch nucleotide sequences based on ENA accession numbers and positions?
Feb
10
awarded  Popular Question
Feb
6
comment Elution of biotinylated RNA
@golgicik Cleaving at a different point is the easiest way if you don't need the biotin later and only use it for purification. I can't comment on the practical aspect as I haven't done something like that myself.
Feb
6
answered Elution of biotinylated RNA
Feb
4
awarded  Famous Question
Jan
23
revised Gel electrophoresis after RNAse treatment
edited title
Jan
23
answered Gel electrophoresis after RNAse treatment
Jan
19
reviewed Needs Improvement How predictably will RNA polymerase not translate repetitive sequences?
Jan
19
reviewed Satisfactory Calculation of saturation of carbon in surface waters
Jan
19
reviewed Satisfactory pfu Turbo DNA polymerase AD
Jan
19
reviewed Excellent Hardy Weinberg Equilibrium problem
Jan
19
reviewed Satisfactory Cats, Dogs and Bears - how are they related?
Jan
19
reviewed Satisfactory What does orbital mean in orbital frontal cortex?
Jan
19
reviewed Satisfactory Why are transmembrane proteins difficult to crystallise?