Shinta

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seen Apr 17 '12 at 16:50

Apr
17
awarded  Editor
Apr
17
revised Measuring reverse transcriptase activity in E. coli
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Apr
17
comment Measuring reverse transcriptase activity in E. coli
Thanks for the reply. (1) I should have mentioned that it is RT from HIV-1. (2) I would like to quantify the RT activity in a number of conformations of RT (mutant RTs).(3) I did not quite get why it may not be expressed after the integration? (4) I do not have any idea how frequent the incorporation into the plasmid by other means occurs - any estimate? (5) E.coli uptake of the RT is indeed an issue - but I suppose E.coli can handle the viral RT?
Apr
17
comment Measuring reverse transcriptase activity in E. coli
Thanks for the reply.
Apr
17
awarded  Student
Apr
17
asked Measuring reverse transcriptase activity in E. coli